Rapid Communication
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Aug 21, 2006; 12(31): 5021-5027
Published online Aug 21, 2006. doi: 10.3748/wjg.v12.i31.5021
Administration of granulocyte colony stimulating factor after liver transplantation leads to an increased incidence and severity of ischemic biliary lesions in the rat model
Olaf Dirsch, Haidong Chi, Yuan Ji, Yan Li Gu, Christoph E Broelsch, Uta Dahmen
Olaf Dirsch, Institute of Pathology, University Hospital Cologne, Joseph-Stelzmann Strasse 9, D-50931 Cologne, Germany
Haidong Chi, Yuan Ji, Yan Li Gu, Christoph E Broelsch, Uta Dahmen, Department of General, Visceral and Transplantation Surgery, University Hospital Essen, Hufelandstrasse 55, D-45122 Essen, Germany
Author contributions: All authors contributed equally to the work.
Supported by the Deutsche Forschungsgemeinschaft (KFO 117/1) and the IFORES Research Program, University Hospital Essen
Correspondence to: Dr. Uta Dahmen, MD, PhD, Department of General, Visceral and Transplantation Surgery, University Hospital Essen, Hufelandstrasse 55, D-45122, Essen, Germany. utadahmen@uni-due.de
Telephone: +49-201-7231121
Received: March 1, 2006
Revised: March 20, 2006
Accepted: March 27, 2006
Published online: August 21, 2006
Abstract

AIM: Recently it has been reported that granulocyte colony stimulating factor (G-CSF) can induce hypercoagulability in healthy bone marrow donors. It is conceivable that the induction of a prothrombotic state in a recipient of an organ graft with already impaired perfusion might cause further deterioration in the transplanted organ. This study evaluated whether G-CSF treatment worsens liver perfusion following liver transplantation in the rat model.

METHODS: A non-arterialized rat liver transplantation model was employed to evaluate the effect of G-CSF treatment on the liver in a syngeneic and allogeneic strain combination. Study outcomes included survival time and liver damage as investigated by liver enzymes and liver histology. Observation times were 1 d, 1 wk and 12 wk.

RESULTS: Rats treated with G-CSF had increased incidence and severity of biliary damage following liver transplantation. In these animals, hepatocellular necrosis was accentuated in the centrilobular region. These lesions are indicative of impaired perfusion in G-CSF treated animals.

CONCLUSION: G-CSF should be used with caution in recipients of liver transplantation, as treatment might enhance preexisting, undetected perfusion problems and ultimately lead to ischemia induced biliary complications.

Keywords: Granulocyte colony stimulating factor; Ischemic biliary lesions; Hypercoagulability; Liver transplantation