Clinical Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Aug 7, 2006; 12(29): 4706-4709
Published online Aug 7, 2006. doi: 10.3748/wjg.v12.i29.4706
Correlation of p53 gene mutation and expression of P53 protein in cholangiocarcinoma
Xiao-Fang Liu, Hao Zhang, Shi-Guang Zhu, Xian-Ting Zhou, Hai-Long Su, Zheng Xu, Shao-Jun Li
Xiao-Fang Liu, Shi-Guang Zhu, Xian-Ting Zhou, Hai-Long Su, Zheng Xu, Shao-Jun Li, Department of Hepatobiliary Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, Shangdong Province, China
Hao Zhang, Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Supported by a grant from Outstanding Youth Foundation of Shandong Province, China, No. 2005BS02008
Correspondence to: Dr. Xiao-Fang Liu, Department of Hepatobiliary Surgery of Yantai Yuhuangding Hospital, Yantai 264000, Shangdong Province, China. liu634@263.net
Telephone: +86-535-6691999 Fax: +86-535-6240341
Received: April 3, 2006
Revised: April 8, 2006
Accepted: April 24, 2006
Published online: August 7, 2006
Abstract

AIM: To characterize the tumor suppressor gene p53 mutations and study the correlation of p53 gene mutation and the expression of P53 protein in cholangiocarcinoma.

METHODS: A total of 36 unselected, frozen samples of cholangiocarcinoma were collected. p53 gene status(exon 5-8) and P53 protein were examined by automated sequencing and immunohistochemical staining, combined with the clinical parameters of patients.

RESULTS: p53 gene mutations were found in 22 of 36 (61.1%) patients. Nineteen of 36 (52.8%) patients were positive for P53 protein expression. There were significant differences in extent of differentiation and invasion between the positive and negative expression of P53 protein. However, there were no significant differences in pathologic parameters between the mutations and non-mutations.

CONCLUSION: The alterations of the p53 gene evaluated by DNA sequence analysis is relatively accurate. Expression of P53 protein could not act as an independent index to estimate the prognosis of cholangiocarcinoma.

Keywords: Cholangiocarcinoma, p53 gene, Mutation, DNA sequence