Colorectal Cancer
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Aug 7, 2006; 12(29): 4660-4664
Published online Aug 7, 2006. doi: 10.3748/wjg.v12.i29.4660
Expression of NOS and HIF-1α in human colorectal carcinoma and implication in tumor angiogenesis
Jian-Xian Yu, Lin Cui, Qi-Yi Zhang, Hua Chen, Ping Ji, Hong-Jun Wei, Hai-Yan Ma
Jian-Xian Yu, Lin Cui, Hua Chen, Hong-Jun Wei, Ping ji, Department of Pathology, Qingdao Municipal Hospital, Qingdao 266011, Shandong Province, China
Qi-Yi Zhang, Department of Internal Medicine, Qingdao Municipal Hospital, Qingdao 266011, Shandong Province, China
Hai-Yan Ma, Department of Pediatrics, Qingdao Municipal Hospital, Qingdao 266011, Shandong Province, China
Supported by the Science Department of Qingdao City, No. 03-1-NY-14-2
Correspondence to: Dr. Jian-Xian Yu, Department of Pathology, Qingdao Municipal Hospital, Qingdao 266011, Shandong Province, China. yujianxian@qingdaonews.com
Telephone: +86-532-82789236 Fax: +86-532-82789233
Received: January 13, 2006
Revised: January 28, 2006
Accepted: February 18, 2006
Published online: August 7, 2006
Abstract

AIM: To study CD34, CD105, inducible nitric oxide synthase (iNOS), endogenous nitric oxide synthase (eNOS), and hypoxia-inducible factor 1 (HIF-1) α expression in human colorectal carcinomas.

METHODS: The tissue microarrays (TMAs) were made up of 80 cases of colorectal carcinoma and 80 cases of non-neoplasm colorectal mucosa. The expression of CD34, CD105, NOS and HIF-1α was detected by immunohistochemistry (S-P).

RESULTS: iNOS and HIF-1α expression in colorectal carcinoma was significantly higher than in non-neoplasm colorectal mucosa (χ2 = 43.166, P < 0.01; χ2 = 10.4278, P < 0.01); eNOS expression in colorectal carcinoma was significantly lower than in non-neoplasm colorectal mucosa (χ2 = 11.354, P < 0.01). The expression of iNOS correlated with differentiation (χ2 = 18.141, P < 0.01), invasive depth (χ2 = 4.748, P < 0.01), and Micro vessel density (MVD) (t = 2.327, P < 0.05). The expression of HIF-1α was correlated with infiltrating depth (χ2 = 4.397, P < 0.05), Duke’s staging (χ2 = 4.255, P < 0.05), and MVD (t = 2.272, P < 0.05). No correlation was found in eNOS expression.

CONCLUSION: Over-expression of iNOS and HIF-1α in colorectal carcinoma is correlated with the biological character MVD.

Keywords: Nitric oxide synthase; HIF-1α; Colorectal carcinoma; Angiogenesis