Published online Aug 7, 2006. doi: 10.3748/wjg.v12.i29.4660
Revised: January 28, 2006
Accepted: February 18, 2006
Published online: August 7, 2006
AIM: To study CD34, CD105, inducible nitric oxide synthase (iNOS), endogenous nitric oxide synthase (eNOS), and hypoxia-inducible factor 1 (HIF-1) α expression in human colorectal carcinomas.
METHODS: The tissue microarrays (TMAs) were made up of 80 cases of colorectal carcinoma and 80 cases of non-neoplasm colorectal mucosa. The expression of CD34, CD105, NOS and HIF-1α was detected by immunohistochemistry (S-P).
RESULTS: iNOS and HIF-1α expression in colorectal carcinoma was significantly higher than in non-neoplasm colorectal mucosa (χ2 = 43.166, P < 0.01; χ2 = 10.4278, P < 0.01); eNOS expression in colorectal carcinoma was significantly lower than in non-neoplasm colorectal mucosa (χ2 = 11.354, P < 0.01). The expression of iNOS correlated with differentiation (χ2 = 18.141, P < 0.01), invasive depth (χ2 = 4.748, P < 0.01), and Micro vessel density (MVD) (t = 2.327, P < 0.05). The expression of HIF-1α was correlated with infiltrating depth (χ2 = 4.397, P < 0.05), Duke’s staging (χ2 = 4.255, P < 0.05), and MVD (t = 2.272, P < 0.05). No correlation was found in eNOS expression.
CONCLUSION: Over-expression of iNOS and HIF-1α in colorectal carcinoma is correlated with the biological character MVD.