Liver Cancer
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Aug 7, 2006; 12(29): 4646-4651
Published online Aug 7, 2006. doi: 10.3748/wjg.v12.i29.4646
Cross-species hybridization of woodchuck hepatitis virus-induced hepatocellular carcinoma using human oligonucleotide microarrays
Paul W Anderson, Bud C Tennant, Zhenghong Lee
Paul W Anderson, Zhenghong Lee, Department of Radiology, University Hospitals of Cleveland, Cleveland, OH 44106, United States
Bud C Tennant, Gastrointestinal Unit, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States
Supported by an NIH grant CA095307 (Z. Lee, PI) and by the Gene Expression Array Core Facility of the Comprehensive Cancer Center of Case Western Reserve University and University Hospitals of Cleveland, No. P30 CA43703
Correspondence to: Zhenghong Lee, Department of Radiology, University Hospitals of Cleveland, Cleveland, OH 44106, United States. zxl11@case.edu
Telephone: +1-216-8447920 Fax: +1-216-8443106
Received: December 24, 2005
Revised: December 28, 2005
Accepted: January 24, 2006
Published online: August 7, 2006
Abstract

AIM: To demonstrate the feasibility of using woodchuck samples on human microarrays, to provide insight into pathways involving positron emission tomography (PET) imaging tracers and to identify genes that could be potential molecular imaging targets for woodchuck hepatocellular carcinoma.

METHODS: Labeled cRNA from woodchuck tissue samples were hybridized to Affymetrix U133 plus 2.0 GeneChips®. Ten genes were selected for validation using quantitative RT-PCR and literature review was made.

RESULTS: Testis enhanced gene transcript (BAX Inhibitor 1), alpha-fetoprotein, isocitrate dehydrogenase 3 (NAD+) beta, acetyl-CoA synthetase 2, carnitine palmitoyltransferase 2, and N-myc2 were up-regulated and spermidine/spermine N1-acetyltransferase was down-regulated in the woodchuck HCC. We also found previously published results supporting 8 of the 10 most up-regulated genes and all 10 of the 10 most down-regulated genes.

CONCLUSION: Many of our microarray results were validated using RT-PCR or literature search. Hence, we believe that woodchuck HCC and non-cancerous liver samples can be used on human microarrays to yield meaningful results.

Keywords: Cross-species hybridization, Gene expression, Woodchuck hepatitis virus, Hepatocellular carcinoma, Woodchuck, Marmota monax