Colorectal Cancer
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jul 21, 2006; 12(27): 4304-4309
Published online Jul 21, 2006. doi: 10.3748/wjg.v12.i27.4304
E-cadherin expression pattern in primary colorectal carcinomas and their metastases reflects disease outcome
Adam Elzagheid, Annika Ålgars, Riyad Bendardaf, Hanan Lamlum, Raija Ristamaki, Yrjo Collan, Kari Syrjanen, Seppo Pyrhonen
Adam Elzagheid, Annika Ålgars, Riyad Bendardaf, Hanan Lamlum, Raija Ristamaki, Yrjo Collan, Kari Syrjanen, Seppo Pyrhonen, Department of Oncology and Radiotherapy, Turku University Hospital, Savitehtaankatu 1 PB 52, FIN-20521 Turku, Finland; Department of Pathology, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland
Supported by grants from the Special Government Funding (EVO) allocated to Turku University Central Hospital
Correspondence to: Dr. Adam Elzagheid, Department of Pathology, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland. adibel@utu.fi
Telephone: +358-2-3133966 Fax: +358-2-3133965
Received: March 29, 2005
Revised: April 28, 2005
Accepted: April 30, 2005
Published online: July 21, 2006
Abstract

AIM: To investigate the changes that occur in E-cadherin expression during the process of metastasis in colorectal cancer.

METHODS: E-cadherin expression was detected by immunohistochemistry and two indices of expression were calculated which reflected the level of expression and the locations (membrane and cytoplasm). Univariate and multivariate survival analyses were used to assess the value of these two E-cadherin indices as predictors of both disease-free (DFS) and disease-specific (DSS) survival.

RESULTS: E-cadherin membrane index (MI), but not cytoplasmic index (CI), was significantly higher in primary tumors than their metastases (P = 0.0001). Furthermore, both primary tumor MI and CI were higher among the patients who developed subsequent metastasis (P = 0.022 and P = 0.007, respectively). Interestingly, both indices were higher in liver metastase compared to other anatomic sites (MI, P = 0.034 and CI, P = 0.022). The CI of the primary tumors was a significant predictor of DFS (P = 0.042, univariate analysis), with a strong inverse correlation between CI and DFS (P = 0.006, multivariate analysis). Finally, the MI of primary tumor proved to be a significant independent predictor of DSS, with higher indices being associated with a more favorable outcome (P = 0.016).

CONCLUSION: Examination of E-cadherin expression and distribution in colorectal tumors can be extremely valuable in predicting disease recurrence. The observation that aberrant cytoplasmic expression of E-cadherin can predict disease recurrence is obviously of great importance for both patients and clinicians, and significantly affects decisions concerning the therapy and management of the patients.

Keywords: Colorectal carcinoma; E-Cadherin membrane; Cytoplasmic immunohistochemistry; Prognosis; Disease-free survival; Disease-specific survival