Rapid Communication
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World J Gastroenterol. Jul 14, 2006; 12(26): 4206-4210
Published online Jul 14, 2006. doi: 10.3748/wjg.v12.i26.4206
Comparison of invasive methods and two different stool antigen tests for diagnosis of H pylori infection in patients with gastric bleeding
Ebru Demiray, Özlem Yılmaz, Cihat Şarkış, Müjde Soytürk, İlkay Şimşek
Ebru Demiray, Özlem Yılmaz, Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Dokuz Eylül University, Inciraltı 35340 Izmir, Turkey
Cihat Şarkış, Müjde Soytürk, İlkay Şimşek, Department of Gastroenterology, Faculty of Medicine, Dokuz Eylül University, Inciraltı 35340 Izmir, Turkey
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Özlem Yilmaz, Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Dokuz Eylül University, Inciraltı 35340 Izmir, Turkey. ozlem.yilmaz@deu.edu.tr
Telephone: +90-232-4124506
Received: December 13, 2005
Revised: January 7, 2006
Accepted: January 14, 2006
Published online: July 14, 2006
Abstract

AIM: To compare two different H pylori stool antigen tests as noninvasive diagnostic methods.

METHODS: The study population consisted of 22 upper gastrointestinal system bleeding patients. Urea breath test (UBT), rapid urease test (RUT) and histopathological examination were applied to all patients. Stool specimens from these patients were examined by rapid STRİP!HpSA and one step simple H pylori antigen cassette test for the detection of H pylori antigens.

RESULTS: For these 22 patients, 15 (68.2%) were diagnosed as positive and seven (31.8%) were diagnosed negative for H pylori infection by the gold standard methods. Whereas 10 (45.5%) were positive and 12 (54.5%) were diagnosed negative by the rapid STRİP!HpSA test. The sensitivity, specificity, positive and negative predictive values were 60%, 86%, 90% and 50%, respectively. When compared to the gold standard methods, these differences were not significant. However, six patients (27.3%) were positive, and 16 (72.7%) were negative by the simple H pylori stool antigen cassette test. The sensitivity, specificity, positive and negative predictive values were 33%, 86%, 83% and 38%, respectively. Compared to the gold standard methods, the simple H pylori stool antigen cassette test results were significantly different (P = 0.012).

CONCLUSION: Rapid STRİP!HpSA test could be used as a routine diagnostic tool in the microbiology laboratory for assessing clinical significance and eradication control of H pylori in upper gastrointestinal system bleeding patients.

Keywords: H pylori; Stool antigen test; Rapid STRİP!HpSA; Simple H pylori antigen cassette test