Colorectal Cancer
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jul 14, 2006; 12(26): 4137-4142
Published online Jul 14, 2006. doi: 10.3748/wjg.v12.i26.4137
Effects of inositol hexaphosphate on proliferation of HT-29 human colon carcinoma cell line
Ying Tian, Yang Song
Ying Tian, Yang Song, Qingdao University Medical College, 38 Dengzhou Road, Qingdao 266021, Shandong Province, China
Author contributions: All authors contributed equally to the work.
Supported by Qingdao Science and Technology Bureau (to Yang Song)
Correspondence to: Professor Yang Song, Qingdao University Medical College, 38 Dengzhou Road, Qingdao 266021, Shandong Province, China. qdsongyang@126.com
Telephone: +86-532-82991029
Received: August 18, 2005
Revised: October 15, 2005
Accepted: October 26, 2005
Published online: July 14, 2006
Abstract

AIM: To investigate the effects of inositol hexaphosphate (IP6) on proliferation of HT-29 human colon carcinoma cell line.

METHODS: Cells were exposed to various concen-trations (0, 1.8, 3.3, 5.0, 8.0, 13.0 mmol/L) of IP6 for a certain period of time. Its effect on growth of HT-29 cells was measured by MTT assay. The expressions of cell cycle regulators treated with IP6 for 2 d were detected by immunocytochemistry.

RESULTS: IP6 inhibited the HT-29 cell growth in a dose- and time-dependent manner. Analysis of cell cycle regulator expression revealed that IP6 reduced the abnormal expression of P53 and PCNA and induced the expression of P21.

CONCLUSION: IP6 has potent inhibitory effect on proliferation of HT-29 cells by modulating the expression of special cell cycle regulators.

Keywords: Phytic Acid; Colonic neoplasms; Cell proliferation