Gastric Cancer
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jun 28, 2006; 12(24): 3810-3813
Published online Jun 28, 2006. doi: 10.3748/wjg.v12.i24.3810
Effects of recombinant human growth hormone on growth of human gastric carcinoma xenograft model in nude mice
Dao-Ming Liang, Jia-Yong Chen, Yi Zhang, Ping Gan, Jie Lin, An-Bao Chen
Dao-Ming Liang, Jia-Yong Chen, Yi Zhang, Ping Gan, Jie Lin, An-Bao Chen, Department of Emergency Surgery of the Second Affiliated Hospital, Kunming Medical College, Kunming 650101, Yunnan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Natural Science Foundation of China, No. 36460133
Correspondence to: Dr. Jia-Yong Chen, Department of Emergency Surgery of Second Affiliated Hospital, Kunming Medical College, Kunming 650101, Yunnan Province, China. cjy125@163.com
Telephone: +86-871-5352825 Fax: +86-871-5352087
Received: November 16, 2005
Revised: February 15, 2006
Accepted: February 19, 2006
Published online: June 28, 2006
Abstract

AIM: To study effects of recombinant human growth hormone (rhGH) on growth of a human gastric carcinoma cell in vivo.

METHODS: Experimental mice were divided into control group, rhGH group, oxaliplatin (L-OHP) group and rhGH+L-OHP group. Cultured human gastric carcinoma cells BGC823 were inoculated into right axilla of nude mice and carcinoma xenograft model was established successfully. Inhibitory rate of xenograft tumor growth was estimated by measuring tumor volume; expression of proliferating cell nuclear antigen (PCNA), Bax and Bcl-2 proteins of xenograft tumor was detected using immunohistochemical S-P method.

RESULTS: Tumor growth inhibitory rate, the positive expression rate of PCNA, Bax and Bcl-2 were 49.3%, 58.2%, 65.2% and 59.2% in rhGH+L-OHP group respectively; 46.6%, 62.5%, 59.7% and 64.7% in L-OHP group; 5.0%, 82.7%, 23.2% and 82.2% in rhGH group and 0, 77.8%, 23.5% and 80.3% in control group. There was significant difference between rhGH+L-OHP group (or L-OHP group ) and control group or rhGH group (P < 0.05), whereas there were no significant differences (P > 0.05) between L-OHP group and rhGH+L-OHP group and between rhGH group and control group.

CONCLUSION: rhGH does not accelerate the proli-feration of human gastric cancer cell in vivo.

Keywords: Human growth hormone; Stomach neoplasm; Nude mice