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World J Gastroenterol. Jun 21, 2006; 12(23): 3766-3769
Published online Jun 21, 2006. doi: 10.3748/wjg.v12.i23.3766
Loss of heterozygosity and microsatellite instabilities of fragile histidine triad gene in gastric carcinoma
Yu-Ping Xiao, Dong-Ying Wu, Lei Xu, Yan Xin
Yu-Ping Xiao, Dong-Ying Wu, Lei Xu, Cancer Insititute, No.1 Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Yan Xin, Fourth Laboratory of Cancer Institute, No.1 Hospital, China Medical University, Shenyang 110001, Liaoning Province, China
Supported by the National Natural Science Foundation of China, No.30070845 and No.30371607
Correspondence to: Professor Yan Xin, Fourth Laboratory of Cancer Institute, No.1 Hospital, China Medical University, Shenyang 110001, Liaoning Province, China. yxin@mail.cmu.edu.cn
Telephone: +86-24-23256666-6351
Received: November 16, 2005
Revised: November 28, 2005
Accepted: January 14, 2006
Published online: June 21, 2006
Abstract

AIM: To detect the loss of heterozygosity (LOH) and microsatellite instabilities (MSI) of fragile histidine triad (FHIT) gene in gastric carcinoma and to study their association with the clinical pathological characteristics of gastric carcinoma.

METHODS: LOH and MSI of FHIT gene were detected at four microsatellite loci D3Sl3H, D3S4l03, D3Sl48l and D3S1234 using PCR in matched normal and cancerous tissues from 50 patients with primary gastric cancer.

RESULTS: The average frequency of LOH and MSI of FHIT gene in gastric cancer was 32.4% and 26.4% respectively. LOH and MSI of FHIT gene in gastric cancer had no association with histological, Borrmann, and Lauren’s classification. LOH of FHIT gene in gastric cancer was related to invasive depth. The frequency of FHIT LOH in gastric cancer with serosa-penetration was obviously higher than that in gastric cancer without serosa-penetration (73.5% vs 37.5%, P < 0.05). MSI of FHIT gene in gastric cancer was associated with the lymph node metastasis. The frequency of MSI in gastric cancer without lymph node metastasis was significantly higher than that in gastric cancer with lymph node metastasis (66.7% vs 34.3%, P < 0.05).

CONCLUSION: LOH of FHIT gene is correlated with invasive depth of gastric carcinoma. MSI of FHIT gene is correlated with lymph node metastases. LOH and MSI of FHIT gene play an important role in carcinogenesis of gastric cancer.

Keywords: Gastric cancer; FHIT gene; Microsatellite instabilities