Detection of gelatinase B activity in serum of gastric cancer patients

doi:10.3748/wjg.v12.i1.105

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Jan 7, 2006 (publication date) through Nov 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 7, 2006; 12(1): 105-109
Published online Jan 7, 2006. doi: 10.3748/wjg.v12.i1.105

Detection of gelatinase B activity in serum of gastric cancer patients

Vesna V Dragutinović, Nebojša S Radovanović, Lidija T Izrael-Živković, Miroslav M Vrvić

Vesna V Dragutinović, Lidija T Izrael-Živković, Institute of Chemistry, School of Medicine, University of Belgrade, Višegradska 26, 11 000 Belgrade, Serbia and Montenegro

Nebojša S Radovanović, Institute of Digestive Diseases, Center of Esophagogastric Surgery, Clinical Center of Serbia, Koste Todorovića 6, 11 000 Belgrade, Serbia and Montenegro

Miroslav M Vrvić, Faculty of Chemistry, University of Belgrade, Studentski trg 16, 11 001 Belgrade, Serbia and Montenegro

Supported by the Ministry of Science and Environment Protection of the Republic of Serbia, No. 1740, TR-6845B and TD-7032B

Correspondence to: Vesna V. Dragutinović, Institute of Chemistry, School of Medicine, University of Belgrade, Višegradska 26, 11 000 Belgrade, Serbia and Montenegro. vdragu@ptt.yu

Telephone: +381-113615773 Fax: +381-113615764

Received: May 18, 2005
Revised: May 28, 2005
Accepted: June 18, 2005
Published online: January 7, 2006

Abstract

AIM: To determine the proteolytic activity and expression of gelatinase B in serum of gastric cancer patients and their correlation with the stage of the tumor.

METHODS: Sera from 23 patients who underwent surgery for primary gastric cancer as the experimental group and from 11 as the control group were used to determine the proteolytic activity and its inhibition by EDTA and 1,10-phenanthroline. Gelatinase B activity was detected by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and SDS-PAGE zymography.

RESULTS: Proteolytic enzyme activity was increased in gastric cancer patients when compared to the control group (P<0.05). The proteinases were determined to be metalloproteinases upon inhibition test with specific metalloproteinase inhibitors 1,10-phenanthroline (P<0.05) and EDTA (P <0.01). SDS-PAGE and SDS-PAGE zymography revealed gelatinase B (proMMP-9) activity and its molecular mass of 92 ku.

CONCLUSION: Proteinase activity is overexpressed in serum of gastric cancer patients. Gelatinase B in serum plays an important role in the progression of gastric cancer. ProMMP-9 can be used as a marker for invasiveness of gastric cancer.

Keywords: Matrix metalloproteinase-9; Gastric cancer; Proteolytic activity; Inhibition