Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 21, 2005; 11(7): 1005-1010
Published online Feb 21, 2005. doi: 10.3748/wjg.v11.i7.1005
Role of adhesion molecules and dendritic cells in rat hepatic/renal ischemia-reperfusion injury and anti-adhesive intervention with anti-P-selectin lectin-EGF domain monoclonal antibody
Tong Zhou, Gui-Zhi Sun, Ming-Jun Zhang, Jin-Lian Chen, Dong-Qing Zhang, Qing-Shen Hu, Yu-Ying Chen, Nan Chen
Tong Zhou, Gui-Zhi Sun, Nan Chen, Department of Nephrology, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, China
Ming-Jun Zhang, Animal Laboratory, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, China
Jin-Lian Chen, Department of Gastroenterology, Shanghai Sixth People’s Hospital, Shanghai 200233, China
Dong-Qing Zhang, Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai 200025, China
Qing-Shen Hu, Yu-Ying Chen, Institute of Cellular Biology, Shanghai Second Medical University, Shanghai 200025, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Tong Zhou, Department of Nephrology, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, China. zhoutong_cn@hotmail.com
Telephone: +86-21-64370045 Fax: +86-21-64333548
Received: February 14, 2004
Revised: February 16, 2004
Accepted: March 2, 2004
Published online: February 21, 2005
Abstract

AIM: To investigate the role of P-selectin, intercellular adhesion molecule-1 (ICAM-1) and dendritic cells (DCs) in liver/kidney of rats with hepatic/renal ischemia-reperfusion injury and the preventive effect of anti-P-selectin lectin-EGF domain monoclonal antibody (anti-PsL-EGFmAb) on the injury.

METHODS: Rat models of hepatic and renal ischemia-reperfusion were established. The rats were then divided into two groups, one group treated with anti-PsL-EGFmAb (n = 20) and control treated with saline (n = 20). Both groups were subdivided into four groups according to reperfusion time (1, 3, 6 and 24 h). The sham-operated group (n = 5) served as a control group. DCs were observed by the microscopic image method, while P-selectin and ICAM-1 were analyzed by immunohistochemistry.

RESULTS: P-selectin increased significantly in hepatic sinusoidal endothelial cells and renal tubular epithelial cells 1 h after ischemia-reperfusion, and the expression of ICAM-1 was up-regulated in hepatic sinusoid and renal vessels after 6 h. CD1a+CD80+DCs gradually increased in hepatic sinusoidal endothelium and renal tubules and interstitium 1 h after ischemia-reperfusion, and there was the most number of DCs in 24-h group. The localization of DCs was associated with rat hepatic/renal function. These changes became less significant in rats treated with anti-PsL-EGFmAb.

CONCLUSION: DCs play an important role in immune pathogenesis of hepatic/renal ischemia-reperfusion injury. Anti-PsL-EGFmAb may regulate and inhibit local DC immigration and accumulation in liver/kidney.

Keywords: Adhesion molecules; Dendritic cells; Hepatic/renal ischemia-reperfusion injury; Anti-P-selectin lectin-EGF domain monoclonal antibody