Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 14, 2005; 11(6): 854-857
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.854
Relationship between polymorphism of class II transactivator gene promoters and chronic hepatitis B
Ying-Ren Zhao, Ling Gong, Ying-Li He, Fang Liu, Chang Lu
Ying-Ren Zhao, Ling Gong, Ying-Li He, Fang Liu, Chang Lu, Department of Infectious Diseases, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30270597
Correspondence to: Dr. Ying-Ren Zhao, Department of Infectious Diseases, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. zhaoyingren@sohu.com
Telephone: +86-29-85323634 Fax: +86-29-85323647
Received: April 6, 2004
Revised: April 9, 2004
Accepted: May 29, 2004
Published online: February 14, 2005
Abstract

AIM: To investigate the relationship between the polymorphism of class II transactivator (CIITA) gene promoters and chronic hepatitis B (CHB).

METHODS: Genomic DNA was prepared from peripheral blood leukocytes. Promoters I, III and IV of gene were analyzed respectively with polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) in 65 patients with CHB, 26 patients with acute hepatitis B (AHB) and 85 normal controls.

RESULTS: No abnormal migration was found in PCR-SSCP analysis of the three promoters in the three groups. Also, no sequential difference was observed at the three promoters among the CHB patients, AHB patients and normal controls.

CONCLUSION: No polymorphism in promoters I, III and IV of CIITA gene exists in CHB patients, ABH patients and normal controls, suggesting that the promoter of CIITA gene might be a conserved domain.

Keywords: Class II transactivator gene promoter; Hepatitis B