Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 7, 2005; 11(5): 744-747
Published online Feb 7, 2005. doi: 10.3748/wjg.v11.i5.744
Association of cyclin D1, p16 and retinoblastoma protein expressions with prognosis and metastasis of gallbladder carcinoma
Hong-Bing Ma, Hai-Tao Hu, Zheng-Li Di, Zuo-Ren Wang, Jing-Sen Shi, Xi-Jing Wang, Yi Li
Hong-Bing Ma, Xi-Jing Wang, Yi Li, Department of Cancer Surgery, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
Hai-Tao Hu, Department of Human Anatomy, Medical College of Xi’an Jiaotong University, Xi’an 710068, Shaanxi Province, China
Zheng-Li Di, Department of Neurology, Xi’an Center Hospital, Xi’an 710003, Shaanxi Province, China
Zuo-Ren Wang, Jing-Sen Shi, Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Hong-Bing Ma, Department of Oncology, Second Hospital, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China. mhbmali@yahoo.com.cn
Telephone: +86-29-87679789
Received: February 27, 2004
Revised: March 1, 2004
Accepted: April 13, 2004
Published online: February 7, 2005
Abstract

AIM: To investigate the role of cyclin D1, p16 and retinoblastoma in cancerous process of gallbladder carcinomas and to assess the relation between cyclin D1, p16, Rb and the biological characteristics of gallbladder carcinoma.

METHODS: Forty-one gallbladder carcinoma, 7 gallbladder adenoma and 14 chronic cholecystitis specimens were immunohistochemically and histopathologically investigated for the relation of cyclin D1, p16 and Rb with Nevin staging and pathologic grading.

RESULTS: The expression rates of abnormal cyclin D1 in gallbladder carcinoma (68.3%)and gallbladder adenoma(57.1%) were significantly higher than those in chronic cholecystitis (7.1%) (P<0.05). No significant difference was found both among the pathological grades G1, G2 and G3 and among Nevin stagings S1-S2, S3 and S4-S5 of gallbladder carcinoma. The positive rates of p16 (48.8%) and Rb (58.5%) in gallbladder carcinoma were significantly lower compared to those in adenoma (100.0%) and cholecystitis (100.0%) (P<0.05). The positive rates of p16 and Rb in Nevin stagings S1-S2 (80.0% and 90.0%) and S3 (46.2% and 61.5%) gallbladder carcinomas were significantly higher than those in S4-S5 (33.3% and 38.8%) (P<0.05), and those in pathologic grades G1 (54.5% and 81.8%) and G2 (50.0% and 62.5%) gallbladder carcinoma were significantly higher than those in G3 (28.6% and 35.7%) (P<0.05). The protein expression of p16 and Rb had a negative-correlation in gallbladder carcinoma (r = -0.2993, P<0.05), and this negative-correlation was correlated with Nevin staging (P<0.05). Moreover, the protein expression of p16 and cyclin D1 had a negative-correlation in gallbladder carcinoma (r = -0.9417, P<0.05).

CONCLUSION: Cyclin D1 may play a role in the early stage of gallbladder carcinoma. Mutation of p16 and Rb genes might be correlated with progression of gallbladder carcinoma. Analysis of p16 and Rb can estimate the prognosis of gallbladder carcinoma. Expression of p16 and Rb may be correlated with Nevin staging and pathologic grading in gallbladder carcinoma.

Keywords: Gallbladder carcinoma, Cyclin D1, p16 protein, Retinoblastoma protein, Tumor metastasis