Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 7, 2005; 11(5): 705-707
Published online Feb 7, 2005. doi: 10.3748/wjg.v11.i5.705
Tiam1 gene expression and its significance in colorectal carcinoma
Li Liu, De-Hua Wu, Yan-Qing Ding
Li Liu, Yan-Qing Ding, Department of Pathology, First Military Medical University, Guangzhou 510515, Guangdong Province, China
De-Hua Wu, Department of Radiation Oncology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30370649
Correspondence to: Dr. Yan-Qing Ding, Department of Pathology, First Military Medical University, Guangzhou 510515, Guangdong Province, China. dyq@fimmu.com
Telephone: +86-20-61642148 Fax: +86-20-61642148
Received: February 14, 2004
Revised: February 17, 2004
Accepted: March 2, 2004
Published online: February 7, 2005
Abstract

AIM: To explore the expression of Tiam1 gene in colorectal carcinoma and its correlation with tumor metastasis.

METHODS: Expressions of Tiam1 gene in 8 colorectal carcinoma cell lines were detected by reverse transcriptase-polymerase chain reaction. In vitro invasiveness was determined by means of Matrigel invasion assay. The correlation of Tiam1 expression with the invasive ability was also analyzed.

RESULTS: Tiam1 gene was highly expressed in LoVo and SW620, which were established from metastatic colorectal carcinomas in comparison with LS174T, SW480, HCT116, LST, HRT-18 and Hee8693, which were established from primary colorectal carcinomas. In vitro cell invasivion demonstrated that LoVo and SW620 had a higher invasive ability than LS174T, SW480, HCT116, LST, HRT-18 and Hee8693. The expression of Tiam1 gene was highly related to the metastatic potential of colorectal carcinoma cells.

CONCLUSION: Tiam1 gene may play an important role in invasion and metastasis of colorectal carcinoma and is a metastasis-related gene.

Keywords: Colorectal carcinoma; Tiam1 gene; Gene expression; Tumor metastasis