Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 14, 2005; 11(46): 7272-7276
Published online Dec 14, 2005. doi: 10.3748/wjg.v11.i46.7272
Effects of ethanol on antioxidant capacity in isolated rat hepatocytes
Sien-Sing Yang, Chi-Chang Huang, Jiun-Rong Chen, Che-Lin Chiu, Ming-Jer Shieh, Su-Jiun Lin, Suh-Ching Yang
Sien-Sing Yang, Liver Unit, Cathay General Hospital, Taipei 106, Taiwan, China
Chi-Chang Huang, Graduate Institute of Pharmacy, School of Pharmacy, Taipei Medical University, Taipei 110, Taiwan, China
Jiun-Rong Chen, Che-Lin Chiu, Ming-Jer Shieh, Suh-Ching Yang, School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan, China
Su-Jiun Lin, Graduate Institute of Biology and Environment Science, School of Cellular and Molecular Biology, University of New Haven, CT 06516, United States
Author contributions: All authors contributed equally to the work.
Supported by the Research Fund from Cathay General Hospital in Taiwan, 93CGH-TMU-15
Correspondence to: Dr Suh-Ching Yang, School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, 250 Wu-Hsing Street, Taipei 110, Taiwan, China. sokei@tmu.edu.tw
Telephone: +886-2-27361661 Fax: +886-2-27373112
Received: March 18, 2005
Revised: September 9, 2005
Accepted: September 12, 2005
Published online: December 14, 2005
Abstract

AIM: To investigate dose-response and time-course of the effects of ethanol on the cell viability and antioxidant capacity in isolated rat hepatocytes.

METHODS: Hepatocytes were isolated from male adult Wistar rats and seeded into 100-mm dishes. Hepatocytes were treated with ethanol at concentrations between 0 (C), 10 (E10), 50 (E50), and 100 (E100) mmol/L (dose response) for 12, 24, and 36 h (time course). Then, lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) concentration, glutathione (GSH) level, and activities of glutathione peroxidase (GPX), glutathione reductase (GRD), superoxide dismutase (SOD), and catalase (CAT) were measured.

RESULTS: Our data revealed that LDH leakage was significantly increased by about 30% in group E100 over those in groups C and E10 at 24 and 36 h, The MDA concentration in groups C, E10 and E50 were significantly lower than that in group E100 at 36 h. Furthermore, the concentration of MDA in group E100 at 36 h was significantly higher by 4.5- and 1.7-fold, respectively, than that at 12 and 24 h. On the other hand, the GSH level in group E100 at 24 and 36 h was significantly decreased, by 32% and 28%, respectively, compared to that at 12 h. The activities of GRD and CAT in group E100 at 36 h were significantly less than those in groups C and E10. However, The GPX and SOD activities showed no significant change in each group.

CONCLUSION: These results suggest that long-time incubation with higher concentration of ethanol (100 mmol/L) decreased the cell viability by means of reducing GRD and CAT activities and increasing lipid peroxidation.

Keywords: Hepatocyte; Ethanol; Lipid peroxidation; Antioxidant capacity