Upregulation of 25-hydroxyvitamin D3-1&alpha;-hydroxylase by butyrate in Caco-2 cells

doi:10.3748/wjg.v11.i45.7136

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 11, Issue 45

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (2215)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-3) series.

Item

Count

PDF

297

HTML

1777

Figures (1-3)

141

Sum=2215

Dec 7, 2005 (publication date) through Apr 30, 2024

Times Cited of This Article

Times Cited (7)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Rapid Communication

World J Gastroenterol. Dec 7, 2005; 11(45): 7136-7141
Published online Dec 7, 2005. doi: 10.3748/wjg.v11.i45.7136

Upregulation of 25-hydroxyvitamin D₃-1α-hydroxylase by butyrate in Caco-2 cells

Oliver Schröder, Sinan Turak, Carolin Daniel, Tanja Gaschott, Jürgen Stein

Oliver Schröder, Sinan Turak, Carolin Daniel, Tanja Gaschott, Jürgen Stein, I^st Department of Internal Medicine, Division of Gastroenterology and Clinical Nutrition, ZAFES, Johann Wolfgang Goethe-University, Frankfurt, Germany

Author contributions: All authors contributed equally to the work.

Supported by the Else Kröner-Fresenius Foundation, Bad Homburg, Germany

Correspondence to: Dr Oliver Schröder, I^st Department of Internal Medicine, Division of Gastroenterology and Clinical Nutrition, ZAFES, Johann Wolfgang Goethe-University, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany. o.schroeder@em.uni-frankfurt.de

Telephone: +49-69-6301-6204 Fax: +49-69-6301-83112

Received: January 12, 2005
Revised: April 1, 2005
Accepted: April 2, 2005
Published online: December 7, 2005

Abstract

AIM: To investigate the possible involvement of 25-hydroxyvitamin D3-1α-hydroxylase [1α-25(OH)2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells.

METHODS: Caco-2 cells were incubated either with 3 mmol/L butyrate and 1 µmol/L 25(OH)₂D₃ or with 1 µmol/L 1α-25(OH)₂D₃ for various time intervals ranging from 0 to 72 h. Additionally, cells were co-incubated with butyrate and either 25(OH)₂D₃ or 1α-25(OH)₂D₃. 1α-25(OH)₂D₃ mRNA was determined semi-quantitatively using the fluorescent dye PicoGreen. Immunoblotting was used for the detection of 1α-25(OH)₂D₃ protein. Finally, enzymatic activity was measured by ELISA.

RESULTS: Both butyrate and 1α-25(OH)₂D₃ stimulated differentiation of Caco-2 cells after a 48 h incubation period, while 25(OH)₂D₃ had no impact on cell differentiation. Synergistic effects on differentiation were observed when cells were co-incubated with butyrate and vitamin D metabolite. Butyrate transiently upregulated 1α-25(OH)₂D₃ mRNA followed by a timely delayed protein upregulation. Coincidently, enzymatic activity was enhanced significantly. The induction of the enzyme allowed for comparable differentiating effects of both vitamin D metabolites.

CONCLUSION: Our experimental data provide a further mechanism for the involvement of the vitamin D signaling pathway in colonic epithelial cell differentiation by butyrate. The enhancement of 1α-25(OH)₂D₃ followed by antiproliferative effects of the vitamin D prohormone in the Caco-2 cell line suggest that 25(OH)₂D₃ in combination with butyrate may offer a new therapeutic approach for the treatment of colon cancer.

Keywords: 25-Hydroxyvitamin D₃-1α-hydroxylase, Butyrate, Caco-2 cells, Colon cancer, Differentiation, Vitamin D