Gastric Cancer
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 14, 2005; 11(42): 6593-6600
Published online Nov 14, 2005. doi: 10.3748/wjg.v11.i42.6593
Polymorphisms of DNA repair genes XRCC1 and XRCC3, interaction with environmental exposure and risk of chronic gastritis and gastric cancer
Márcia Cristina Duarte, Jucimara Colombo, Andrea Regina Baptista Rossit, Alaor Caetano, Aldenis Albaneze Borim, Durval Wornrath, Ana Elizabete Silva
Márcia Cristina Duarte, Jucimara Colombo, Ana Elizabete Silva, UNESP - São Paulo State University, Department of Biology, Campus São José do Rio Preto, SP, Brazil
Andrea Regina Baptista Rossit, FAMERP- São José do Rio Preto School of Medicine, Microorganism Investigation Center, São José do Rio Preto, SP, Brazil
Alaor Caetano, Aldenis Albaneze Borim, FAMERP- São José do Rio Preto School of Medicine, Hospital de Base, São José do Rio Preto, SP, Brazil
Durval Wornrath, Pio XII Foundation, Barretos, SP, Brazil
Author contributions: All authors contributed equally to the work.
Supported by Brazilian Agency CAPES
Correspondence to: Ana Elizabete Silva, Departamento de Biologia, UNESP, Rua Cristóvão Colombo, 2265, Jardim Nazareth, CEP: 15054-000, São José do Rio Preto, SP, Brazil. anabete@ibilce.unesp.br
Telephone: +55-17-32212384 Fax: +55-17-32212390
Received: February 24, 2005
Revised: April 6, 2005
Accepted: April 9, 2005
Published online: November 14, 2005
Abstract

AIM: To evaluate the association between poly-morphisms XRCC1 Arg194Trp and Arg399Gln and XRCC3 Thr241Met and the risk for chronic gastritis and gastric cancer, in a Southeastern Brazilian population.

METHODS: Genotyping by PCR-RFLP was carried out on 202 patients with chronic gastritis (CG) and 160 patients with gastric cancer (GC), matched to 202 (C1) and 150 (C2) controls, respectively.

RESULTS: No differences were observed among the studied groups with regard to the genotype distribution of XRCC1 codons 194 and 399 and of XRCC3 codon 241. However, the combined analyses of the three variant alleles (194Trp, 399Gln and 241Met) showed an increased risk for chronic gastritis when compared to the GC group. Moreover, an interaction between the polymorphic alleles and demographic and environmental factors was observed in the CG and GC groups. XRCC1 194Trp was associated with smoking in the CG group, while the variant alleles XRCC1 399Gln and XRCC3 241Met were related with gender, smoking, drinking and H pylori infection in the CG and GC groups.

CONCLUSION: Our results showed no evidence of a rela-tionship between the polymorphisms XRCC1 Arg194Trp and Arg399Gln and XRCC3 Thr241Met and the risk of chronic gastritis and gastric cancer in the Brazilian population, but the combined effect of these variants may interact to increase the risk for chronic gastritis, considered a premalignant lesion. Our data also indicate a gene-environment interaction in the susceptibility to chronic gastritis and gastric cancer.

Keywords: Gastric cancer; Gastritis; XRCC1; XRCC3; Polymorphism; Environmental exposure