Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 28, 2005; 11(4): 551-556
Published online Jan 28, 2005. doi: 10.3748/wjg.v11.i4.551
Effect of cholecystokinin on experimental neuronal aging
Xiao-Jiang Sun, Qin-Chi Lu, Yan Cai
Xiao-Jiang Sun, Department of Neurology and Neurobiology Laboratory, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China
Qin-Chi Lu, Yan Cai, Department of Neurology and Neurobiology Laboratory, Renji Hospital Affiliated to Shanghai Second Medical University, Shanghai 200001, China
Author contributions: All authors contributed equally to the work.
Supported by Shanghai Natural Science Foundation, No. 9821314040
Correspondence to: Xiao-Jiang Sun, Ph. D. Professor, Department of Neurology and Neurobiology Laboratory, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiaotong University, No. 600 Yishan Road, Shanghai 200233, China. sunxj155@sohu.com
Telephone: +86-21-64369181-8363 Fax: +86-21-64368920
Received: January 2, 2004
Revised: January 4, 2004
Accepted: February 24, 2004
Published online: January 28, 2005
Abstract

AIM: To observe the effect of cholecystokinin (CCK) on lipofusin value, neuronal dendrite and spine ultrastructure, and total cellular protein during the process of experimental neuronal aging.

METHODS: Experimental neuronal aging study model was established by NBA2 cellular serum-free culture method. By using single intracellular lipofusin value from microspectrophotometry, morphology of neuronal dendrites and spines from the scanner electron microscopy, and total cellular protein as the indexes of experimental neuronal aging, we observed the effect of CCK8 on the process of experimental neuronal aging.

RESULTS: Under the condition of serum-free culture, intracellular fluorescence value (%) increased with the extension of culture time (1 d 8.51±3.43; 5 d 10.12±3.03; 10 d 20.54±10.3; 15 d 36.88±10.49; bP<0.01). When CCK was added to serum-free culture medium, intracellular lipofusin value (%) decreased remarkably after consecutive CCK reaction for 10 and 15 d (control 36.88±10.49; 5 d 32.03±10.01; 10 d 14.37±5.55; 15 d 17.31±4.80; bP<0.01). As the time of serum-free culturing was prolonged, the number of neuronal dendrite and spine cells decreased. The later increased in number when CCK8 was added. CCK8 could improve the total cellular protein in the process of experimental neuronal aging.

CONCLUSION: CCK8 may prolong the process of experimental neuronal aging by maintaining the structure and the number of neuronal dendrite and spine cells and changing the total cellular protein.

Keywords: Cholecystokinin, Neuron, Cell Aging