Leelawat K, Engprasert S, Tujinda S, Suthippintawong C, Enjoji M, Nakashima M, Watanabe T, Leardkamolkarn V. Receptor-binding cancer antigen expressed on SiSo cells can be detected in metastatic lymph nodes from gastrointestinal cancers. World J Gastroenterol 2005; 11(38): 6014-6017 [PMID: 16273616 DOI: 10.3748/wjg.v11.i38.6014]
Corresponding Author of This Article
Kawin Leelawat, MD, PhD, Department of Surgery, Rajavithi Hospital, Rajavithi Road, Rajathevi, Bangkok 10400, Thailand. kawin@tubtim.cri.or.th
Article-Type of This Article
Brief Reports
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Kawin Leelawat, Department of Surgery, Rajavithi Hospital, Bangkok 10400, Thailand
Kawin Leelawat, Vijittra Leardkamolkarn, Department of Anatomy, Mahidol University, Bangkok 10400, Thailand
Surang Engprasert, Department of Pharmacognosy, Kyushu University, Fukuoka 8128582, Japan
Supathip Tujinda, Cheepsumon Suthippintawong, Department of Pathology, Rajavithi Hospital, Bangkok 10400, Thailand
Munechika Enjoji, Manabu Nakashima, Takeshi Watanabe, Department of Medical Bioregulation, Kyushu University, Fukuoka 8128582, Japan
Author contributions: All authors contributed equally to the work.
Supported by the Thailand Research Fund in the Royal Golden Jubilee Program
Correspondence to: Kawin Leelawat, MD, PhD, Department of Surgery, Rajavithi Hospital, Rajavithi Road, Rajathevi, Bangkok 10400, Thailand. kawin@tubtim.cri.or.th
Telephone: +66-2-245-6078 Fax: +66-2-245-6075
Received: February 2, 2004 Revised: March 1, 2004 Accepted: March 2, 2004 Published online: October 14, 2005
Abstract
AIM: To investigate the expression of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) in metastatic lymph nodes from gastrointestinal cancer.
METHODS: Metastatic lymph nodes from gastrointestinal cancer were detected for RCAS1 by immunohistochemical staining and mRNA in situ hybridization.
RESULTS: A total of 102 metastatic lymph nodes from bile duct, gastric, colon, and pancreatic cancer were investigated for RCAS1 expression. The immunoreactivity of RCAS1 was identified in 100% of metastatic lymph nodes. Both local and distant metastatic lymph nodes showed RCAS1 expression. On the contrary, specimens of non-cancerous lymph nodes were negative for RCAS1. The result of mRNA in situ hybridization was also confirmed by the finding of immunohistochemical staining. RCAS1 mRNA was detected in all tumor cells that metastasized to lymph nodes.
CONCLUSION: All metastatic lymph nodes express RCAS1 in tumor cells at both protein and mRNA levels, and RCAS1 should be used as a complementary factor for identification of metastatic lymph nodes from gastrointestinal cancers.
