Colorectal Cancer
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 28, 2005; 11(36): 5655-5658
Published online Sep 28, 2005. doi: 10.3748/wjg.v11.i36.5655
B7 molecule mRNA expression in colorectal carcinoma
Ju-Xiang Xiao, Pei-Song Bai, Bao-Chang Lai, Li Li, Juan Zhu, Yi-Li Wang
Ju-Xiang Xiao, Pei-Song Bai, Li Li, Juan Zhu, Department of Oncology, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Bao-Chang Lai, Yi-Li Wang, Institute for Cancer research, School of Life Science and Technology of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30370549
Correspondence to: Dr. Ju-Xiang Xiao, Department of Oncology, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. xiao_juxiang@yahoo.com
Telephone: +86-29-85324068 Fax: +86-29-85237369
Received: May 13, 2005
Revised: June 2, 2005
Accepted: June 6, 2005
Published online: September 28, 2005
Abstract

AIM: To observe the status of tumor-associated B7 molecule mRNA expression in human colorectal cancer tissue by in situ hybridization.

METHODS: The mRNA expression patterns of cancer-associated B7-1,B7H1,B7H2,ICOS in 22 specimens of human colorectal cancer tissue were monitored by in situ hybridization (ISH) with digoxin-labeled oligonucleotide probes.

RESULTS: B7-1,B7H1,B7H2,ICOS mRNA were detected in both cancer cells and tumor infiltrating lymphocytes (TIL). The mRNA expression level of these molecules in tumor cells was higher than that in TIL (0.76 ± 0.54-1.62 ± 0.82 vs 0.38 ± 0.19-0.65 ± 0.33, P<0.001). There was no relationship between expression level of tested B7 family molecules and patients’ sex, age, differentiation status of cancer and regional lymph node metastasis.

CONCLUSION: Th2 cytokine predominant in tumor microenvironment might be related to the expression of B7H1,B7H2 co-signal molecules in tumor cells and TIL.

Keywords: Colorectal cancer; B7-1, B7H1, B7H2 and ICOS; Tumor immunity; Immune evasion