Gastric Cancer
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 28, 2005; 11(36): 5592-5600
Published online Sep 28, 2005. doi: 10.3748/wjg.v11.i36.5592
DNA ploidy analysis and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma
Jing-Fang Zhang, Yuan-Ping Zhang, Feng-Yun Hao, Cai-Xin Zhang, Yu-Jun Li, Xiang-Rui Ji
Jing-Fang Zhang, Department of Pathology, Taishan Medical University, Taian 271000, Shandong Province, China
Yuan-Ping Zhang, Department of Prosthodontics, Taian Hospital of Stomatology, Taian 271000, Shandong Province, China
Feng-Yun Hao, Department of Pathology, Weifang Municipal People’s Hospital, Weifang 261031, Shandong Province, China
Cai-Xin Zhang, Department of Pathology, Qingdao Municipal Hospital, Qingdao 266003, Shandong Province, China
Yu-Jun Li, Xiang-Rui Ji, Department of Pathology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Bureau of Education of Shandong Province, No. 03K02
Correspondence to: Professor Xiang-Rui Ji, the Affiliated Hospital of Medical College, Qingdao University, 16 Jiangsu Road, Qingdao 266003, Shandong Province, China. jixiangrui@yahoo.com.cn
Telephone: +86-532-2911533
Received: September 10, 2004
Revised: November 1, 2004
Accepted: November 4, 2004
Published online: September 28, 2005
Abstract

AIM: To investigate DNA ploidy and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma and to explore the mechanism of invasion and metastasis of gastric carcinoma.

METHODS: Immunohistochemical methods were used to detect the expressions of MMP-9, TIMP-2, and E-cadherin in 156 cases, including 99 cases of gastric carcinoma, 16 cases of adjacent noncancerous mucosa, 16 cases of distant metastases and 25 cases of metastatic lymph node (LN) from gastric carcinoma. Flow cytometry DNA ploidy and S-phase fraction (SPF) analysis were performed on 57 cases, including 47 cases of gastric cancer, 6 cases of adjacent noncancerous mucosa, and 4 cases of distant metastatic cancer.

RESULTS: The expression of MMP-9 was significantly correlated with Lauren’s classification, Borrmann’s classification, LN metastasis, tumor metastasis, and TNM stage, as well as depth of invasion (all P < 0.05). The positive rate was lower in noncarcinoma than in carcinoma (31.3% vs 66.7%, P < 0.01). The expression of TIMP-2 was significantly correlated with Borrmann’s classification, LN metastasis, and the depth of invasion (all P < 0.05). The expression of E-cadherin was significantly correlated with differentiation, Lauren’s classification, Borrmann’s classification, and LN metastasis, as well as the depth of invasion (P < 0.01 or P < 0.05). E-cadherin was less expressed in carcinoma than in noncarcinoma (42.4% vs 87.5%, P < 0.01). There was a positive correlation between MMP-9 and TIMP-2 and a negative correlation between MMP-9 and E-cadherin, but no correlation between TIMP-2 and E-cadherin. Also there was a positive correlation between DNA aneuploid rate and differentiation and LN metastasis. SPF that was higher than 15% was positively correlated with tumor size, differentiation and LN metastasis. And there was a significant difference between carcinoma and noncarcinoma in DNA aneuploid rate and SPF.

CONCLUSION: With tumor progression and development of heterogeneity, the abnormal expressions of MMP-9, TIMP-2, and E-cadherin or DNA aneuploid rate or high SPF gradually increases, suggesting that they play a crucial role in gastric carcinoma progression.

Keywords: MMP-9; TIMP-2; E-cadherin; DNA ploidy; Gastric carcinoma