Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 28, 2005; 11(24): 3665-3670
Published online Jun 28, 2005. doi: 10.3748/wjg.v11.i24.3665
Changes of gastrointestinal myoelectric activity and bile acid pool size after cholecystectomy in guinea pigs
Xue-Mei Zhang, Lei Dong, Li-Na Liu, Bi-Xia Chang, Qian He, Qian Li
Xue-Mei Zhang, Lei Dong, Li-Na Liu, Bi-Xia Chang, Department of Gastroenterology, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
Qian He, Department of Clinical Lab, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
Qian Li, Center of ultrasonography, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30170414
Correspondence to: Professor Lei Dong, Department of Gastroenterology, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China. zxmzxs0417@163.com
Telephone: +86-29-83243061 Fax: +86-29-87679758
Received: February 24, 2005
Revised: February 25, 2005
Accepted: May 13, 2005
Published online: June 28, 2005
Abstract

AIM: To investigate the bile acid pool size after cholecystectomy whether or not correlated to the gastrointestinal migrating myoelectric complex (MMC) in guinea pigs.

METHODS: Gallbladder motilities were assessed before cholecystectomy. Furthermore, we continuously monitored interdigestive gastrointestinal motilities using bipolar electrodes in conscious guinea pigs before and after surgery at 4 wk in standard diet group and high cholesterol diet (cholesterol gallstone) group. Total bile acid pool sizes were measured by isotope dilution method at meantime.

RESULTS: After cholecystectomy, there were parallel falls in duration of phase I, II, III and MMC cycle duration but increase in amplitude in the guinea pigs with normal gallbladder function, and in the guinea pigs with cholesterol stones. However, There were not significantly differences. On the other hand, the bile acid pool was definitely small in the GS guinea pigs compared to normal guinea pigs and became slightly smaller after cholecystectomy. Similarly, bile acid in gallbladder bile, fecal bile acid was slightly increased in GS guinea pigs after cholecystectomy, to the same degree as normal. These differences, however, were not significant.

CONCLUSION: It is concluded that in the guinea pigs with normal gallbladder function, and in the guinea pigs with cholesterol stones: (1) Cholecystectomy produce a similar but less marked trend in bile acid pool; and (2) MMC are linked to enterohepatic circulation of bile acids, rather than surgery, which is consistent with changes of the bile acid pool size. As a result, gastrointestinal dyskinesia is not involved in occurrence of postchole-cystectomy syndrome.

Keywords: Cholecystectomy; Enterohepatic circulation of bile acids; Bile acid pool; Migrating motor complex; Gallbladder motility