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©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 21, 2005; 11(23): 3591-3594
Published online Jun 21, 2005. doi: 10.3748/wjg.v11.i23.3591
Published online Jun 21, 2005. doi: 10.3748/wjg.v11.i23.3591
Expression of CD44v6 gene in normal human peripheral blood
Jian Song, Dong-Sheng Zhang, Jie Zheng, Department of Pathology, Institute of Molecular Pathology, Medical School of Southeast University, Nanjing 210009, Jiangsu Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Jie Zheng, Department of Pathology, Medical School of Southeast University, Nanjing 210009, Jiangsu Province, China. jiezheng@public1.ptt.js.cn
Telephone: +86-25-83272358 Fax: +86-25-83324887
Received: August 26, 2003
Revised: August 28, 2003
Accepted: September 23, 2003
Published online: June 21, 2005
Revised: August 28, 2003
Accepted: September 23, 2003
Published online: June 21, 2005
Abstract
AIM: To investigate if CD44v6 could be used as a molecular marker of cancer progression and metastasis through the detection of CD44v6 gene expression in normal human peripheral blood.
METHODS: RNA was extracted from the peripheral blood mononuclear cells of 50 healthy donors, the expression of CD44v6 was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTS: CD44v6 mRNA was detected in 58% of healthy volunteers under the proper controls.
CONCLUSION: Our results suggest that the measurement of CD44v6 expression in peripheral blood by RT-PCR is not suitable for detection of circulating tumor cells.
Keywords: CD44v6, Peripheral blood, Tumor marker