Mechanism and clinical significance of cyclooxygenase-2 expression in gastric cancer

doi:10.3748/wjg.v11.i21.3240

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 11, Issue 21

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (3129)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-2) series, Tables (1-2) series.

Item

Count

PDF

284

HTML

2500

Figures (1-2)

171

Tables (1-2)

174

Sum=3129

Jun 7, 2005 (publication date) through Nov 5, 2024

Times Cited of This Article

Times Cited (9)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Jun 7, 2005; 11(21): 3240-3244
Published online Jun 7, 2005. doi: 10.3748/wjg.v11.i21.3240

Mechanism and clinical significance of cyclooxygenase-2 expression in gastric cancer

Bao-Can Wang, Chang-Qing Guo, Chao Sun, Qiao-Ling Sun, Guo-Yong Liu, Ding-Guo Li

Bao-Can Wang, Chao Sun, Qiao-Ling Sun, Ding-Guo Li, Digestive Department, Xinhua Hospital, Shanghai Second Medical University, Shanghai 200092, China

Chang-Qing Guo, Guo-Yong Liu, Digestive Department, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Ding-Guo Li, Digestive Department, Xinhua Hospital, Shanghai Second Medical University, 1665 Kongjiang Road, Shanghai 200092, China. dingguo-li@hotmail.com

Telephone: +86-21-65790000 Fax: +86-21-65795173

Received: March 23, 2004
Revised: March 24, 2004
Accepted: June 11, 2004
Published online: June 7, 2005

Abstract

AIM: To determine the correlation between methylation status of 5’ CpG island of cyclooxygenase-2 (COX-2) gene and protein expression in gastric cancer tissues for distinguishing the molecular characters of gastric cancers.

METHODS: Methylation status of 5’ CpG island of COX-2 gene was studied by PCR amplification after HpaII and Hha I restrictive enzyme digestion; COX-2 expression was evaluated by immunohistochemical method.

RESULTS: Hpa II and HhaI site were all methylated in 12 normal gastric mucosa tissues, whereas they were demethylated in 77.27% (34/44) and 84.09% (37/44) gastric cancer tissues, respectively. Expression of COX-2 was detected in 68.18% (30/44) gastric cancer tissues, but no expression was found in normal gastric mucosa tissues. In gastric cancer tissues, COX-2 expression was correlated significantly with HpaII site demethylation (29/30 vs 5/14, P<0.001 and HhaI site demethylation (28/30 vs 9/14, P<0.05).

CONCLUSION: The demethylation of 5’ CpG island of gene is necessary for COX-2 expression in human gastric cancer. The expression status of COX-2 may provide theoretical basis for COX-2 targeting gastric cancer treatments.

Keywords: Gastric cancer; Methylation; Cyclooxygenase-2