Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 14, 2005; 11(18): 2830-2833
Published online May 14, 2005. doi: 10.3748/wjg.v11.i18.2830
Gastric mucosal injury due to hemorrhagic reperfusion and efficacy of Salvia miltiorrhizae extract F and cimetidine
Li-Hong Zhang, Chang-Bai Yao, Ming-Qi Gao, He-Quan Li
Li-Hong Zhang, Department of Anesthesiology, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Chang-Bai Yao, Department of General Surgery, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Ming-Qi Gao, Function Laboratory Center, China Medical University, Shenyang 110004, Liaoning Province, China
He-Quan Li, Department of Pathophysiology, China Medical University, Shenyang 110004, Liaoning Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Li-Hong Zhang, Department of Anesthesiology, Second Affiliated Hospital, China Medical University, 36 Sanhao Street, Shenyang 110004, Liaoning Province, China. cbyao@126.com
Telephone: +86-13304014888
Received: March 18, 2004
Revised: March 19, 2004
Accepted: April 27, 2004
Published online: May 14, 2005
Abstract

AIM: To observe the gastric mucosal injury caused by hemorrhagic shock and reperfusion and to compare the effect between Salvia miltiorrhizae extract F (SEF) and cimetidine (CI) on it.

METHODS: A model of hemorrhage/reperfusion injury was produced by Itoh method. Wistar rats were randomly divided into three groups: 0.9% sodium chloride treatment group (NS group), SEF treatment group (SEF group), and CI treatment group (CI group). Saline, SEF and CI were injected respectively. The index of gastric mucosal lesions (IGML) was expressed as the percentage of lesion area in the gastric mucosa. The degree of gastric mucosal lesions was categorized into grades 0, 1, 2, 3. Atom absorption method was used to measure the intracellular calcium content. Radioimmunoassay was used to measure the concentrations of prostaglandins.

RESULTS: IGML (%) and grade 3 (%) were 23.18±6.82, 58.44±9.07 in NS group, 4.42±1.39, 20.32±6.95 in SEF group and 3.74±1.56, 23.12±5.09 in CI group, and the above parameters in SEF group and CI group decreased significantly (IGML: SEF vs NS, t = 6.712, P = 0.000<0.01; CI vs NS, t = 6.943, P = 0.000<0.01; grade 3: SEF vs NS, t = 8.386, P = 0.000; CI vs NS, t = 8.411, P = 0.000), but the grade 0 and grade 1 damage in SEF group (22.05±5.96, 34.12±8.12) and CI group (18.54±4.82, 30.15±7.12) were markedly higher than those in NS group (3.01±1.01, 8.35±1.95; grade 0: SEF vs NS, t = 8.434, P = 0.000<0.01; CI vs NS, t = 7.950, P = 0.000<0.01; grade 1: SEF vs NS, t = 8.422, P = 0.000<0.01; CI vs NS, t = 8.448, P = 0.000<0.01). The intracellular calcium content (μg/mg) in SEF group (0.104±0.015) and CI group (0.102±0.010) was markedly lower than that in NS group (0.131±0.019, SEF vs NS, t = 2.463, P = 0.038<0.05; CI vs NS, t = 3.056, P = 0.017<0.05). The levels (pg/mg) of PGE2, 6-keto-PGF and 6-keto-PGF/TXB2 were 540±183, 714±124, 17.38±5.93 in NS group and 581±168, 737±102, 19.04±8.03 in CI group, 760±192, 1 248±158, 33.42±9.24 in SEF group, and the above parameters in SEF group markedly raised (PGE2: SEF vs NS, t = 2.282, P = 0.046<0.05; SEF vs CI, t = 2.265, P = 0.047<0.05; 6-keto-PGF: SEF vs NS, t = 6.583, P = 0.000<0.000; SEF vs CI, t = 6.708, P = 0.000<0.01; 6-keto-PGF/TXB2: SEF vs NS, t = 3.963, P = 0.003<0.001; SEF vs CI, t = 3.243, P = 0.009<0.01), whereas TXB2 level in SEF group (45.37±7.54) was obviously lower than that in NS group (58.28±6.74, t = 3.086, P = 0.014<0.05) and CI group (54.32±6.89, t = 2.265, P = 0.047<0.05). No significant difference was shown between NS group and CI group (PGE2: t = 0.414, P = 0.688>0.05; 6-keto-PGF: t = 0.310, P = 0.763>0.05; TXB2: t = 1.099, P = 0.298>0.05; 6-keto-PGF/TXB2: t = 0.372, P = 0.718>0.05).

CONCLUSION: Both SEF and CI could inhibit reperfusion-induced injury in gastric mucosa, but with different mechanisms. SEF could not only enhance the protective effect of gastric mucosa, but also abate the injury factors, while CI can only abate the injury factors.

Keywords: Hemorrhagic shock; Reperfusion injury; Gastric mucosa; Radix Salvia miltiorrhizae; Cimetidine