Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 7, 2005; 11(17): 2634-2636
Published online May 7, 2005. doi: 10.3748/wjg.v11.i17.2634
Effects of interferon-alpha on expression of hepatic stellate cell and transforming growth factor-β1 and α-smooth muscle actin in rats with hepatic fibrosis
Xin-Ming Chang, Ying Chang, Ai Jia
Xin-Ming Chang, Ying Chang, Ai Jia, Department of Gastroenterology, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Xin-Ming Chang, Department of Gastroenterology, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Telephone: +86-29-85324051
Received: September 23, 2004
Revised: September 24, 2004
Accepted: November 4, 2004
Published online: May 7, 2005
Abstract

AIM: To investigate the effect of interferon-α (IFN-α) on preventing or reversing hepatic fibrosis in rat experimental model induced by CCl4.

METHODS: One hundred and ten Sprague-Dawley rats were divided into five groups: group A (normal controls, n = 18), group B (fibrotic model controls, n = 22), group C (IFN-α prevention, n = 22) initially treated with intra-muscular injection of IFN-α in saline daily at the doses of 1×105 U for 6 wk, group D (IFN-α treatment, n = 24) treated with intra-muscular injection of IFN-α in saline daily at the doses of 1×105 U for 6 wk after the first 6 wk, group E (0.9% sodium chloride treatment control, n = 24) treated with intra-muscular injection of 0.01 mL/kg daily for 6 wk after the first 6 wk. At the end of the experiment, all rats of each group were killed. Samples of the liver obtained by biopsy were subjected to histological, immunohistochemical and electron microscopic studies for the expressions of transforming growth factor-β1 (TGF- β1) and α-smooth muscle actin (α-SMA).

RESULTS: The expressions of TGF-β1, the number of activated hepatic stellate cells and α-SMA in hepatic tissue of group C were significantly less than those of group B (P<0.01). The degree of fibrosis score in group B was also significantly less than that of group C under light microscope (P<0.01).

CONCLUSION: IFN-α can inhibit the production of TGF-β1, decrease HSC activation and stimulate its apoptosis.

Keywords: Interferon-α; Transforming growth factor-β1; Hepatic stellate cell; Hepatic fibrosis; Apoptosis