Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 21, 2005; 11(15): 2296-2305
Published online Apr 21, 2005. doi: 10.3748/wjg.v11.i15.2296
Identification of expressed genes in regenerating rat liver in 0-4-8-12 h short interval successive partial hepatectomy
Cun-Shuan Xu, Jin-Yun Yuan, Wen-Qiang Li, Hong-Peng Han, Ke-Jin Yang, Cui-Fang Chang, Li-Feng Zhao, Yu-Chang Li, Hui-Yong Zhang, Salman Rahman, Jing-Bo Zhang
Cun-Shuan Xu, Hong-Peng Han, Jin-Yun Yuan, Cui-Fang Chang, Ke-Jin Yang, Li-Feng Zhao, College of Life Science, Henan Normal University, Xinxiang 453002, Henan Province, China
Salman Rahman, Homophilia Research Center, London University, London SE17EH, UK
Wen-Qiang Li, Yu-Chang Li, Hui-Yong Zhang, Jing-Bo Zhang, State Key Laboratory of Cell Differentiation and Regulation of Henan Province and Ministry, Xinxiang 453002, Henan Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Professor Cun-Shuan Xu, College of Life Science, Henan Normal University, Xinxiang 453002, Henan Province, China. xucs@x263.net
Telephone: +86-373-3326001 Fax: +86-373-3326524
Received: March 11, 2004
Revised: March 12, 2004
Accepted: May 29, 2004
Published online: April 21, 2005
Abstract

AIM: To identify the genes differentially expressed in the regenerating rat liver of 0-4-8-12 h short interval successive partial hepatectomy (SISPH) and to analyze their expression profiles.

METHODS: Five hundred and fifty-one elements screened from subtractive cDNA libraries were made into a cDNA microarray (cDNA chip). Extensive gene expression analysis following 0-4-8-12 h SISPH was conducted by microarray.

RESULTS: One hundred and eighty-three elements were selected, which were either up- or down-regulated more than 2-fold at one or more time points after SISPH. Cluster analysis and generalization analysis showed that there were five distinct temporal patterns of gene expression. Eighty-six genes were unreported, associated with liver regeneration (LR).

CONCLUSION: Microarray analysis shows that the down regulated genes are much more than the up-regulated ones in SISPH; the numbers of genes expressed consistently are fewer than that expressed immediately; the genes expressed in high abundance are much fewer than that increased 2-5-fold. The comparison of SISPH with partial hepatectomy (PH) shows that the expression trends of most genes in SISPH and in PH are similar, but the expression of 43 genes is specifically altered in SISPH.

Keywords: SISPH; Liver regeneration