Effect of indomethacin on cell cycle proteins in colon cancer cell lines

doi:10.3748/wjg.v11.i11.1693

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 21, 2005; 11(11): 1693-1696
Published online Mar 21, 2005. doi: 10.3748/wjg.v11.i11.1693

Effect of indomethacin on cell cycle proteins in colon cancer cell lines

Mei-Hua Xu, Gui-Ying Zhang

Mei-Hua Xu, Gui-Ying Zhang, Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China

Author contributions: All authors contributed equally to the work.

Supported by the Ministry of Health, Natural Science Foundation of China, No. 98-2-088

Correspondence to: Dr. Gui-Ying Zhang, Department of Gastroenterology, Xiangya Hospital, Central South University, 141 Xiangya Road, Changsha 410008, Hunan Province, China. meihuaxu2001@yahoo.com.cn

Telephone: +86-731-4327106

Received: October 26, 2004
Revised: October 28, 2004
Accepted: December 9, 2004
Published online: March 21, 2005

Abstract

AIM: To study the effect of indomethacin (IN) on human colon cancer cell line SW480 with p53 mutant and SW480 transfected wild-type p53 (wtp53/SW480) in vitro and investigate molecular mechanism of anti-tumor effect of IN on colon cancer.

METHODS: SW480 cells and wtp53/SW480 cells were treated with different concentrations of IN respectively, the expressions of CDK₂, CDK4 and p21^WAF1/CIP1 protein were detected by Western blotting.

RESULTS: IN gradually down-regulated the expression of CDK₂, CDK4 protein of wtp53/SW480 cells in a dose-dependent manner, and inhibitory effect reached the maximum level at 600 μmol/L; IN up-regulated the expression of p21^WAF1/CIP1 protein in a dose-dependent manner at a certain concentration range, and the expression reached the maximum level at 400 μmol/L, and returned to the base level at 600 μmol/L. The expression of CDK₂, CDK4 and p21^WAF1/CIP1 protein of SW480 cells did not change.

CONCLUSION: IN exerts antitumor effect partly through down regulation of the expression of CDK₂, CDK4 protein and up regulation of the expression of p21^WAF1/PIC1.

Keywords: CDK₂, CDK4, p21^WAF1/CIP1, SW480, Colon cancer