Effect of indomethacin on cell cycle proteins in colon cancer cell lines

doi:10.3748/wjg.v11.i11.1693

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 11, Issue 11

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (2368)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-2) series.

Item

Count

PDF

253

HTML

1893

Figures (1-2)

222

Sum=2368

Mar 21, 2005 (publication date) through Dec 11, 2024

Times Cited of This Article

Times Cited (5)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Brief Reports

World J Gastroenterol. Mar 21, 2005; 11(11): 1693-1696
Published online Mar 21, 2005. doi: 10.3748/wjg.v11.i11.1693

Effect of indomethacin on cell cycle proteins in colon cancer cell lines

Mei-Hua Xu, Gui-Ying Zhang

Mei-Hua Xu, Gui-Ying Zhang, Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China

Author contributions: All authors contributed equally to the work.

Supported by the Ministry of Health, Natural Science Foundation of China, No. 98-2-088

Correspondence to: Dr. Gui-Ying Zhang, Department of Gastroenterology, Xiangya Hospital, Central South University, 141 Xiangya Road, Changsha 410008, Hunan Province, China. meihuaxu2001@yahoo.com.cn

Telephone: +86-731-4327106

Received: October 26, 2004
Revised: October 28, 2004
Accepted: December 9, 2004
Published online: March 21, 2005

Abstract

AIM: To study the effect of indomethacin (IN) on human colon cancer cell line SW480 with p53 mutant and SW480 transfected wild-type p53 (wtp53/SW480) in vitro and investigate molecular mechanism of anti-tumor effect of IN on colon cancer.

METHODS: SW480 cells and wtp53/SW480 cells were treated with different concentrations of IN respectively, the expressions of CDK₂, CDK4 and p21^WAF1/CIP1 protein were detected by Western blotting.

RESULTS: IN gradually down-regulated the expression of CDK₂, CDK4 protein of wtp53/SW480 cells in a dose-dependent manner, and inhibitory effect reached the maximum level at 600 μmol/L; IN up-regulated the expression of p21^WAF1/CIP1 protein in a dose-dependent manner at a certain concentration range, and the expression reached the maximum level at 400 μmol/L, and returned to the base level at 600 μmol/L. The expression of CDK₂, CDK4 and p21^WAF1/CIP1 protein of SW480 cells did not change.

CONCLUSION: IN exerts antitumor effect partly through down regulation of the expression of CDK₂, CDK4 protein and up regulation of the expression of p21^WAF1/PIC1.

Keywords: CDK₂; CDK4; p21^WAF1/CIP1; SW480; Colon cancer