Liu DB, Gu ZD, Cao XZ, Liu H, Li JY. Immunocytochemical detection of HoxD9 and Pbx1 homeodomain protein expression in Chinese esophageal squamous cell carcinomas. World J Gastroenterol 2005; 11(10): 1562-1566 [PMID: 15770739 DOI: 10.3748/wjg.v11.i10.1562]
Corresponding Author of This Article
De-Bin Liu, Department of Thoracic Surgery, Peking University School of Oncology, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing 100036, China. dbliumd@sohu.com
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Brief Reports
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World J Gastroenterol. Mar 14, 2005; 11(10): 1562-1566 Published online Mar 14, 2005. doi: 10.3748/wjg.v11.i10.1562
Immunocytochemical detection of HoxD9 and Pbx1 homeodomain protein expression in Chinese esophageal squamous cell carcinomas
De-Bin Liu, Zhen-Dong Gu, Xiao-Zhe Cao, Hong Liu, Ji-You Li
De-Bin Liu, Zhen-Dong Gu, Hong Liu, Department of Thoracic Surgery, Peking University School of Oncology, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing 100036, China
Xiao-Zhe Cao, Department of Pathology, Lanzhou General Hospital of PLA, Lanzhou 730030, Gansu Province, China
Ji-You Li, Department of Pathology, Peking University School of Oncology, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing 100036, China
Author contributions: All authors contributed equally to the work.
Supported by Beijing New Star of Science and Technology Plan from Beijing Municipal Science and Technology Commission, No. 954813600, and by Institutional Research Fund of Peking University School of Oncology, Beijing Cancer Hospital; and partially supported by Research Fund of Beijing Municipal Science and Technology Commission, No. H020920030390
Correspondence to: De-Bin Liu, Department of Thoracic Surgery, Peking University School of Oncology, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing 100036, China. dbliumd@sohu.com
Telephone: +86-10-88121122 Fax: +86-10-88122437
Received: August 16, 2004 Revised: August 18, 2004 Accepted: October 18, 2004 Published online: March 14, 2005
Abstract
AIM: To evaluate the expression pattern of two novel oncofetal antigens, the HoxD9 and Pbx1 homeoproteins in esophageal squamous cell carcinomas (ESCCs) to determine what role they would play in the carcinogenesis of ESCC.
METHODS: We obtained tissue samples of ESCC from 56 patients who underwent esophagectomy but not preoperative chemotherapy or radiotherapy. The diagnosis of ESCC was established and confirmed by staff pathologists. We used a highly sensitive, indirect, immunocytochemical method to detect HoxD9 and PbX1 proteins. We qualitatively and quantitively evaluated cells that exhibited and staining using a light microscope.
RESULTS: In all observed carcinoma tissue samples, more than 60% of neoplastic cells stained lightly or strongly for HoxD9, and more than 50% of neoplastic cells stained lightly or strongly for Pbx1.
CONCLUSION: Our data suggest that HoxD9 and Pbx1 are inappropriately expressed in most human esophageal squamous cell carcinoma. Understanding the role of Hox genes in esophageal epithelial cell carcinogenesis may not only augment early detection but also offer new avenues for treatment of this disease.
