Gastric Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 1, 2004; 10(9): 1246-1249
Published online May 1, 2004. doi: 10.3748/wjg.v10.i9.1246
Role of nucleostemin in growth regulation of gastric cancer, liver cancer and other malignancies
Si-Jin Liu, Zi-Wei Cai, Ya-Jun Liu, Mei-Yu Dong, Li-Qiu Sun, Guo-Fa Hu, Ying-Yun Wei, Wei-De Lao
Si-Jin Liu, Guo-Fa Hu, Ying-Yun Wei, Wei-De Lao, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
Zi-Wei Cai, Li-Qiu Sun, Institute of Biology of Self-protection, Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang Province, China
Ya-Jun Liu, Department of Spine, Beijing Jishuitan Hospital, Peking University, Beijing 100035, China
Mei-Yu Dong, Tianjin Institute of Urologic Surgery, Tianjin Medical University, Tianjin 300211, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Professor Wei-De Lao, No. 3 Zhongguancun Road, Haidian District, Beijing 100080, China. wdlao@genetics.ac.cn
Telephone: +86-10-62552956 Fax: +86-10-62551951
Received: December 23, 2003
Revised: January 1, 2004
Accepted: January 15, 2004
Published online: May 1, 2004
Abstract

AIM: To examine the role of nucleostemin in the growth regulation of gastric cancer, liver cancer and other cancers.

METHODS: RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells. Eighteen kinds of malignant tumor tissues including gastric adenocarcinoma and liver cancer tissues, 3 kinds of benign tumor tissues, 3 kinds of benign hyperplastic tissues and normal tissues were employed to examine nucleostemin gene expression by RT-PCR, Slot blot, Northern blot and in situ hybridization.

RESULTS: We successfully cloned a 570 bp fragment of nucleostemin-cDNA from HEK-293 cells. All detected malignant tumor tissues, benign tumor tissues, and benign hyperplastic tissues had high levels of nucleostemin expression. Nucleostemin was also expressed in human placenta tissue at a high level. In terminally differentiated normal human adult kidney and mammary gland tissues, no nucleostemin expression could be detected.

CONCLUSION: Nucleostemin can help regulate the proliferation of both cancer cells and stem cells. It might play an important role in the growth regulation of gastric cancer, liver cancer and other cancers.

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