Viral Hepatitis
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 1, 2004; 10(7): 968-971
Published online Apr 1, 2004. doi: 10.3748/wjg.v10.i7.968
Effect of SEN virus coinfection on outcome of lamivudine therapy in patients with hepatitis B
Dong Xu, De-Ying Tian, Zhen-Gang Zhang, Hong-Yun Chen, Pei-Hui Song
Dong Xu, De-Ying Tian, Zhen-Gang Zhang, Hong-Yun Chen, Pei-Hui Song, Department of Infectious Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Science and Technology Commission Foundation of Hubei Province, No. 2002AA301C32
Correspondence to: Dr. De-Ying Tian, Department of Infectious Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Street 1095, Wuhan 430030, Hubei Province, China. xudongtj@tom.com
Telephone: +86-27-83662815 Fax: +86-27-83613408
Received: June 6, 2003
Revised: July 1, 2003
Accepted: July 30, 2003
Published online: April 1, 2004
Abstract

AIM: Interactions between hepatitis B virus (HBV) and other viral hepatitis infections are well known, whether the newly discovered SEN virus (SENV) has any effect on lamivudine antiHBV activity is unclear. Our aim was to clarify the effect on treatment outcome of coinfection with SEN virus in patients with hepatitis B during lamivudine therapy.

METHODS: Nested polymerase chain reaction (PCR) amplification was used to detect SENV-D and SENV-H strains in serum from 45 patients with chronic hepatitis B treated with lamivudine 100 mg daily for 12 mo. HBV DNA load was detected with fluorescence quantitative PCR (FQ-PCR) and YMDD (tyrosine, methionine, aspartate, aspartate) motif mutation of HBV DNA was investigated with cDNA microarray.

RESULTS: SENV DNA was detected in 5 of 45(11.1%) cases after 12 mo they received lamivudine treatment. SENV-D and SENV-H were 4.4% and 6.7% respectively. HBV DNA failed to respond to lamivudine therapy in 4 of 5 SENV coinfected patients while only 10 of 40 patients became SENV positive and the difference was statistically significant. Response of ALT and HBeAg to lamivudine had no significant difference between coinfection patients and single HBV infection ones.

CONCLUSION: Coinfection with SEN virus in chronic hepatitis B patients may adversely affect the outcome of lamivudine treatment.

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