Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 2004; 10(4): 579-582
Published online Feb 15, 2004. doi: 10.3748/wjg.v10.i4.579
A novel method for preparation of tissue microarray
Han-Lei Dan, Ya-Li Zhang, Yan Zhang, Ya-Dong Wang, Zuo-Sheng Lai, Yu-Jie Yang, Hai-Hong Cui, Yan-Ting Jian, Jian Geng, Yan-Qing Ding, Chun-Hai Guo, Dian-Yuan Zhou
Han-Lei Dan, Ya-Li Zhang, Ya-Dong Wang, Zuo-Sheng Lai, Yu-Jie Yang, Hai-Hong Cui, Yan-Ting Jian, Dian-Yuan Zhou, Institute of Digestive Medicine, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China
Yan Zhang, Jian Geng, Yan-Qing Ding, Department of Pathology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China
Chun-Hai Guo, Center Department of Chinese Academy of Engineering and Physics, Mianyang 621000, Sichuan Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Han-Lei Dan, M.D., Institute of Digestive Medicine, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China. henrydan@sina.com
Telephone: +86-20-61341531 Fax: +86-20-61341531
Received: May 11, 2003
Revised: July 8, 2003
Accepted: August 18, 2003
Published online: February 15, 2004
Abstract

AIM: To improve the technique of tissue microarray (tissue chip).

METHODS: A new tissue microarraying method was invented with a common microscope installed with a special holing needle, a sampling needle, and a special box fixing paraffin blocks on the microscope slide carrier. With the movement of microscope tube and objective stage on vertical and cross dimensions respectively, the holing procedure on the recipient paraffin blocks and sampling procedure of core tissue biopsies taken from the donor blocks were performed with the refitted microscope on the same platform. The precise observation and localization of representative regions in the donor blocks were also performed with the microscope equipped with a stereoscope.

RESULTS: Highly-qualified tissue chips of colorectal tumors were produced by a new method, which simplified the conventional microarraying procedure, and was more convenient and accurate than that employing the existing tissue microarraying instruments.

CONCLUSION: Using the refitted common microscope to produce tissue microarray is a simple, reliable, cost-effective and well-applicable technique.

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