Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 2004; 10(4): 560-566
Published online Feb 15, 2004. doi: 10.3748/wjg.v10.i4.560
Effects of Ginkgo biloba extract on cytoprotective factors in rats with duodenal ulcer
Jane C.-J. Chao, Huei-Chen Hung, Sheng-Hsuan Chen, Chia-Lang Fang
Jane C.-J. Chao, Huei-Chen Hung, School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu Hsing Street, Taipei, Taiwan 110, China
Sheng-Hsuan Chen, Department of Gastroenterology, Taipei Medical University Hospital, 252 Wu Hsing Street, Taipei, Taiwan 110, China
Chia-Lang Fang, Department of Pathology, Taipei Medical University, 250 Wu Hsing Street, Taipei, Taiwan 110, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Jane C.-J. Chao, School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu Hsing Street, Taipei, Taiwan 110, China. chenjui@tmu.edu.tw
Telephone: +886-2-2736-1661 #6551~6556 Ext. 117 Fax: +886-2-2737-3112
Received: October 30, 2003
Revised: November 18, 2003
Accepted: December 16, 2003
Published online: February 15, 2004
Abstract

AIM: To investigate the effects of Ginkgo biloba extract on cytoprotective factors in rats with duodenal ulcer.

METHODS: Sprague-Dawley rats were randomly divided into four groups: sham operation without ginkgo, sham operation with ginkgo, duodenal ulcer without ginkgo, and duodenal ulcer with ginkgo. Rats with duodenal ulcer were induced by 500 mL/L acetic acid. Rats with ginkgo were intravenously injected with Ginkgo biloba extract from the tail at a dose of 0.5 mg/(kg·d) for 7 and 14 days.

RESULTS: Pathological result showed that duodenal ulcer rats with ginkgo improved mucosal healing and inflammation compared with those without ginkgo after 7 d treatment. After 14 d treatment, duodenal ulcer rats with ginkgo significantly increased weight gain (34.0 ± 4.5 g versus 24.5 ± 9.5 g, P < 0.05) compared with those without ginkgo. Duodenal ulcer rats significantly increased cell proliferation (27.4 ± 4.0 and 27.8 ± 2.3 BrdU-labeled cells in duodenal ulcer rats with and without ginkgo versus 22.4 ± 3.5 and 20.8 ± 0.5 BrdU-labeled cells in sham operation rats with and without ginkgo, P < 0.05) compared with sham operation rats. Mucosal prostaglandin E2 concentration significantly increased by 129% (P < 0.05) in duodenal ulcer rats with ginkgo compared with that in those without ginkgo. Duodenal ulcer rats without ginkgo significantly decreased superoxide dismutase activity in the duodenal mucosa and erythrocytes (19.4 ± 6.7 U/mg protein versus 38.1 ± 18.9 U/mg protein in the duodenal mucosa, and 4.87 ± 1.49 U/mg protein versus 7.78 ± 2.16 U/mg protein in erythrocytes, P < 0.05) compared with sham operation rats without ginkgo. However, duodenal ulcer rats with ginkgo significantly increased erythrocyte superoxide dismutase activity (8.22 ± 1.92 U/mg protein versus 4.87 ± 1.49 U/mg protein, P < 0.05) compared with those without ginkgo. Duodenal ulcer rats without ginkgo significantly increased plasma lipid peroxides (4.18 ± 1.12 μmol/mL versus 1.60 ± 1.10 μmol/mL and 1.80 ± 0.73 μmol/mL, P < 0.05) compared with sham operation rats without ginkgo and duodenal ulcer rats with ginkgo during the experimental period.

CONCLUSION: Ginkgo biloba extract can improve weight gain and mucosal healing in duodenal ulcer rats by the actions of cytoprotection and antioxidation.

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