Liver Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 2004; 10(4): 509-513
Published online Feb 15, 2004. doi: 10.3748/wjg.v10.i4.509
Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma
Hai Song, Shuang-Luo Xia, Cheng Liao, Yi-Liang Li, Yi-Fei Wang, Tsai-Ping Li, Mu-Jun Zhao
Hai Song, Shuang-Luo Xia, Cheng Liao, Tsai-Ping Li, Mu-Jun Zhao, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institues for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Yi-Liang Li, East China Normal University, Shanghai 200062, China
Yi-Fei Wang, Department of Mathematics, Shanghai University, Shanghai 200436, China
Author contributions: All authors contributed equally to the work.
Supported by the grants from National High Technology Research and Development Program of China (863 Program), No. 2001AA221021 and No. 2002BA711A02
Correspondence to: Professor Mu-Jun Zhao, P.O. Box 35, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institues for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China. mjzhao@sibs. ac.cn
Telephone: +86-21-54921115 Fax: +86-21-54921011
Received: August 23, 2003
Revised: September 2, 2003
Accepted: October 7, 2003
Published online: February 15, 2004
Abstract

AIM: To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray.

METHODS: The 33P labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets, and an expression profile of genes was produced in one paired human liver tumor tissue. After a global analysis of gene expression of 8400 genes, we selected some genes to confirm the differential expression using Northern blot and RT-PCR.

RESULTS: Parallel analysis of the hybridized signals enabled us to get an expression profile of genes in which about 500 genes were differentially expressed in the paired liver tumor tissues. We identified 4 genes, the expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. In addition, the expression of these genes in 6 hepatoma cell lines was also showed by RT-PCR analysis.

CONCLUSION: cDNA microarray permits a high throughput identification of changes in gene expression. The genes encoding Beclin 1, RbAp48, Pir51 and aldolase b are first reported that may be related with hepatocarcinoma.

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