Gastric Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 2004; 10(4): 484-490
Published online Feb 15, 2004. doi: 10.3748/wjg.v10.i4.484
Anti-neoplastic efficacy of Haimiding on gastric carcinoma and its mechanisms
Yu-Bin Ji, Shi-Yong Gao, Hong-Rui Ji, Qi Kong, Xiu-Juan Zhang, Bao-Feng Yang
Yu-Bin Ji, Shi-Yong Gao, Hong-Rui Ji, Qi Kong, Postdoctoral Research Station, Institute of Materia Medica, Harbin Commercial University, Harbin 150076, Heilongjiang Province, China Xiu-Juan Zhang, Department of Pharmaceutical Engineering, Harbin Commercial University, Harbin 150076, Heilongjiang Province, China
Bao-Feng Yang, Department of Pharmacology, Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30271598; Outstanding Post Doctoral Research Foundation of China, No. 17(1999); Heilongjiang Province Foundation for Talented Youth No. J 9906; Natural Science Foundation of Heilongjiang Province, China, No. D00-18; Innovative Fund for Distinguished University and College Teachers of Heilongjiang Province, No. 2001015
Correspondence to: Dr. Yu-Bin Ji, Postdoctoral Research Station, Institute of Materia Medica, Harbin Commercial University, 138 Tongda Street, Daoli District, Harbin 150076, Heilongjiang Province, China. sygao2002@sina.com
Telephone: +86-451-84866001 Fax: +86-451-84866001
Received: May 11, 2003
Revised: May 28, 2003
Accepted: June 7, 2003
Published online: February 15, 2004
Abstract

AIM: To study the anti-neoplastic effect of Haimiding and its mechanisms of action.

METHODS: Experiments using MTT and colony formation were carried out to study the in vitro anti-neoplastic action of Haimiding, its in vivo anti-neoplastic action was studied by observing its effect on the weight of tumors in FC mice and S180, H22 tumor bearing mice, as well as their life spans. The effect of Haimiding on cell apoptosis and different stages of cell cycles in human gastric carcinoma cells were studied by flow cytometry. Its effect on [Ca2+]i of human gastric carcinoma cells and the source of Ca2+ during the change of [Ca2+]i were observed by confocal laser scanning technique.

RESULTS: Haimiding showed a definite cytotoxicity to 8 human tumor cell lines, which was most prominent against BGC-823, Eca-109 and HCT-8 tumor cells. It also exhibited an obvious inhibition on colony formation of the above tumor cell lines, which was most prominent in Eca-109 tumor cells. It showed obvious inhibition on the growth of tumor in FC mice and S180 bearing mice as well as prolonged the life span of H22 bearing mice. It was able to induce apoptosis and elevate intracellular [Ca2+]i concentration of tumor cells. The source of Ca2+ came from both extracellular Ca2+ influx and intracellular Ca2+ release.

CONCLUSION: Haimiding is composed of a TCM preparation and 5-flurouracil. Its anti-neoplastic potency is highly enhanced by synergism as compared with either one of its components. Its mechanisms of anti-neoplastic action can be attributed to its action to initiate apoptosis of tumor cells by opening the membrane calcium channel and inducing intracellular Ca2+ release to elevate [Ca2+]i of the tumor cells.

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