Cloning and sequence analysis of gene oipA encoding an outer membrane protein of human Helicobacter pylori

doi:10.3748/wjg.v10.i21.3205

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Nov 1, 2004; 10(21): 3205-3207
Published online Nov 1, 2004. doi: 10.3748/wjg.v10.i21.3205

Cloning and sequence analysis of gene oipA encoding an outer membrane protein of human Helicobacter pylori

Dao-Rong Chen, Ai-Long Huang, Xiao-Hong Tao, Pi-Long Wang, Zheng Jiang

Dao-Rong Chen, Xiao-Hong Tao, Pi-Long Wang, Zheng Jiang, Department of Gastroenterology, the First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China

Ai-Long Huang, Institute of Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China

Author contributions: All authors contributed equally to the work.

Supported by Fund of Chongqing Health Bureau, No. 001110438

Correspondence to: Dr. Dao-Rong Chen, Department of Gastroenterology, the First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China. cdrcdr@mail.china.com

Telephone: +86-23-68122549

Received: February 14, 2004
Revised: February 28, 2004
Accepted: March 6, 2004
Published online: November 1, 2004

Abstract

AIM: To construct a recombinant E. coli strain that would highly express the proinflammatory outer membrane protein of human Helicobacter pylori (H pylori).

METHODS: The oipA DNA was amplified by PCR, inserted into pET-32a, and transformed into Top10 E. coli strain. This recombinant plasmid of Top10 was sent out for nucleotide sequence analysis. Finally this sequence AF479754 was compared with HP0638 and JHP0581.

RESULTS: The sequence of the aim gene was obtained. It had 924 base pairs. The identity was 95.32% against HP0638, 95.02% against JHP0581, which was higher than the identity between HP0638 and JHP0581.

CONCLUSION: Although the aim gene was obtained, but it was different from the published sequence of GenBank. It is not clear what makes this difference. Maybe it is because different strain was used or because there were some variations. So more researches are required to prove it.

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