Gastric Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 2004; 10(20): 2936-2939
Published online Oct 15, 2004. doi: 10.3748/wjg.v10.i20.2936
Alteration of cyclin D1 in gastric carcinoma and its clinicopathologic significance
Peng Gao, Geng-Yin Zhou, Yuan Liu, Jin-Song Li, Jun-Hui Zhen, Yin-Ping Yuan
Peng Gao, Geng-Yin Zhou, Jin-Song Li, Jun-Hui Zhen, Yin-Ping Yuan, Department of Pathology, School of Medicine, Shandong University, Jinan 250012, Shandong Province, China
Yuan Liu, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30300124
Correspondence to: Peng Gao, Department of Pathology, School of Medicine, Shandong University, Jinan 250012, Shandong Province, China. gaopeng@sdu.edu.cn
Telephone: +86-531-8382045 Fax: +86-531-8382045
Received: November 17, 2003
Revised: December 26, 2003
Accepted: March 18, 2004
Published online: October 15, 2004
Abstract

AIM: To detect the genetic alteration and abnormal expression of cyclin D1 in gastric carcinoma and investigate its clinicopathologic significance in advanced gastric carcinoma.

METHODS: Proteins of cyclin D1 were detected by immunohistochemistry in 42 cases of advanced gastric carcinoma with their follow-up data available, 27 cases of early stage carcinoma, 21 cases of gastric adenoma, 22 cases of hyperplastic polyp and 20 cases of normal mucosa adjacent to adenocarcinomas. Genetic alteration of cyclin D1 was detected by Southern blot and expression of cyclin D1 mRNA was detected by PT-PCR in 42 cases of advanced gastric carcinoma.

RESULTS: Cyclin D1 protein was not expressed in normal mucosa, hyperplastic polyp and gastric adenoma, while it was only positively expressed in gastric carcinoma. The expression rate of cyclin D1 protein in early stage gastric carcinoma, advanced gastric carcinoma and lymph node metastasis was 48.1%, 47.4% and 50.0%, respectively. The amplification of cyclin D1 gene was detected in 16.6% of advanced gastric carcinomas. The overexpression of cyclin D1 mRNA was detected in 40.5% of the samples. There was no significant correlation between cyclin D1 protein expression and age, lymph-node metastasis and histological grading in patients with advanced gastric carcinoma (χ2 = 0.038,0.059,0.241, P > 0.05). Significant correlation was observed between the expression of cyclin D1 protein and the 5-year survival rate (χ2 = 3.92, P < 0.05).

CONCLUSION: Detection of cyclin D1 protein by immunohistochemistry may be useful in the diagnosis of early gastric carcinomas. Patients with positive expression of cyclin D1 protein tend to have a worse prognosis.

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