Liu Z, Sun HX, Zhang YW, Li YF, Zuo J, Meng Y, Fang FD. Effect of SNPs in protein kinase Cz gene on gene expression in the reporter gene detection system. World J Gastroenterol 2004; 10(16): 2357-2360 [PMID: 15285019 DOI: 10.3748/wjg.v10.i16.2357]
Corresponding Author of This Article
Fu-De Fang, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. fangfd@public3.bta.net.cn
Article-Type of This Article
Basic Research
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World J Gastroenterol. Aug 15, 2004; 10(16): 2357-2360 Published online Aug 15, 2004. doi: 10.3748/wjg.v10.i16.2357
Effect of SNPs in protein kinase Cz gene on gene expression in the reporter gene detection system
Zhuo Liu, Hong-Xia Sun, Yong-Wei Zhang, Yun-Feng Li, Jin Zuo, Yan Meng, Fu-De Fang
Zhuo Liu, Hong-Xia Sun, Yong-Wei Zhang, Yun-Feng Li, Jin Zuo, Yan Meng, Fu-De Fang, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
Author contributions: All authors contributed equally to the work.
Supported by the National High Technology Research and Development Program of China, No. 2002BA711A05, No. 2002BA711A10-02 and the National Natural Science Foundation of China, No. 30170441, No. 30370668 and the Natural Science Foundation of Beijing, No. 7032033 and the Foundation of Ministry of Education of China, No. 20030023020, No. 20010023024 Co-first-authors: Zhuo Liu and Yong-Wei Zhang
Correspondence to: Fu-De Fang, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. fangfd@public3.bta.net.cn
Telephone: +86-10-65253005 Fax: +86-10-65253005
Received: December 10, 2003 Revised: January 4, 2004 Accepted: January 12, 2004 Published online: August 15, 2004
Abstract
AIM: To investigated the effects of the SNPs (rs411021, rs436045, rs427811, rs385039 and rs809912) on gene expression and further identify the susceptibility genes of type 2 diabetes.
METHODS: Ten allele fragments (49 bp each) were synthesized according to the 5 SNPs mentioned above. These fragments were cloned into luciferase reporter gene vector and then transfected into HepG2 cells. The activity of the luciferase was assayed. Effects of the SNPs on RNA splicing were analyzed by bioinformatics.
RESULTS: rs427811T allele and rs809912G allele enhanced the activity of the reporter gene expression. None of the 5 SNPs affected RNA splicing.
CONCLUSION: SNPs in protein kinase Cz (PKCZ) gene probably play a role in the susceptibility to type 2 diabetes by affecting the expression level of the relevant genes.
