H Pylori
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 1, 2004; 10(13): 1907-1910
Published online Jul 1, 2004. doi: 10.3748/wjg.v10.i13.1907
Effect of biopsies on sensitivity and specificity of ultra-rapid urease test for detection of Helicobacter pylori infection: A prospective evaluation
Li Lin Lim, Khek Yu Ho, Bow Ho, Manuel Salto-Tellez
Li Lin Lim, Khek Yu Ho, Department of Medicine, National University Hospital, Singapore
Bow Ho, Department of Microbiology, National University Hospital, Singapore
Manuel Salto-Tellez, Department of Pathology, National University Hospital, Singapore
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Khek Yu Ho, Department of Medicine, National University Hospital, 5 Lower Kent Ridge Road, 119074, Singapore. mdchoky@nus.edu.sg
Fax: +65-67794112
Received: October 27, 2003
Revised: February 2, 2004
Accepted: February 9, 2004
Published online: July 1, 2004
Abstract

AIM: To prospectively assess the sensitivity, specificity and time to positivity of theUltra-rapid urease test (URUT) for Helicobacter pylori (H pylori ), and compare the results of one with those of two biopsies.

METHODS: Five antral biopsies were taken in consecutive patients undergoing upper endoscopy: one and two biopsies for URUT, and one each for H pylori culture and histology. URUT was read at 1, 5, 10, 20 and 30 min, 1, 2, 3 and 24 h after biopsy insertion into the reagent. A positive histology and/or culture was used as positive reference ”gold standards”.

RESULTS: URUT was more sensitive for detecting H pylori with two biopsies rather than one, at all time points up to 120 min. The sensitivity improved from 3.6% to 82.1% for one biopsy and 10.7% to 85.7% for two biopsies from 1 to 120 min. The sensitivity reached 96.4% at 24 h for both, but the specificity reduced from 100% to 96% and 92% for one and two biopsies, respectively.

CONCLUSION: Development of a positive URUT result is hastened by doubling the number of gastric biopsies. We recommend taking two instead of one biopsy to achieve an earlier positive URUT result so that H pylori eradication therapy can be initiated before patient is discharged from the endoscopy suite.

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