Liver Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 1, 2004; 10(11): 1560-1564
Published online Jun 1, 2004. doi: 10.3748/wjg.v10.i11.1560
Quantitative detection of common deletion of mitochondrial DNA in hepatocellular carcinoma and hepatocellular nodular hyperplasia
Jian-Yong Shao, Hong-Yi Gao, Yu-Hong Li, Yu Zhang, You-Yong Lu, Yi-Xin Zeng
Jian-Yong Shao, Hong-Yi Gao, Yu-Hong Li, Yu Zhang, Yi-Xin Zeng, Cancer Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong Province, China
You-Yong Lu, Beijing Institute for Cancer Research, Beijing Laboratory of Molecular Oncology, School of Oncology, Peking University, Beijing 100034, China
Author contributions: All authors contributed equally to the work.
Supported by the National Key Basic Science Research Program, Contract No: G1998051201; The Foundation of Guangdong Science and Technology Committee, Contract No: 2003A3080202; and The Foundation of Guangzhou Science and Technology Committee, Contract No: 2003I-E0341
Correspondence to: Jian-Yong Shao, M.D., Ph.D., Department of Pathology, Cancer Center, Sun Yat-Sen University, 651 Dong Feng Road East, Guangzhou 510060, Guangdong Province, China. jyshao@gzsums.edu.cn
Telephone: +86-20-87343391 Fax: +86-20-87343391
Received: August 23, 2003
Revised: October 4, 2003
Accepted: October 12, 2003
Published online: June 1, 2004
Abstract

AIM: To study the deletion of mitochondiral DNA in hepatocellular carcinoma and hepatocellular nodular hyperplasia and its significance in the development of cancer.

METHODS: Deleted mtDNA (CD-mtDNA) and wild type mtDNA (WT-mtDNA) were quantitatively analyzed by using real-time PCR in 27 hepatocellular carcinomas (HCC) and corresponding noncancerous liver tissues and 27 hepatocellular nodular hyperplasiae (HNH).

RESULTS: A novel CD (4981 bp) was detected in 85% (23/27) and 83% (22/27) of HCC and HNH tumor tissues, respectively, which were significantly higher than that in paired noncancerous liver tissues (57%, 15/27) (P < 0.05). The CD/WT-mtDNA ratio in HCC tumors was 0.00092 (median, interquartile range, 0.0001202 - 0.00105), which was significantly higher than that in paired noncancerous liver tissues (median, 0.000, quartile range, 0 - 0) (P = 0.002, Mann-Whitney Test), and was 25 of times of that in HNH tissues (median, 0.0000374, quartile range, 0 - 0.0004225) (P = 0.002, Mann-Whitney test).

CONCLUSION: CD-mtDNA mutation plays an important role in the development and progression of HCC.

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