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KÖSTEK M, DEMİR U, UÇAK R, AVCI BY, ÜNAL A, GÜLÇİÇEK OB, ÇALIŞKAN O, ÇİTGEZ B, SERİN E, YETKİN SG, MİHMANLI M, ULUDAĞ M. Effects of glutamine and omega-3 fatty acids on intestinal neomucosa formation on colon serosa in rats. Turk J Med Sci 2024; 54:59-68. [PMID: 38812652 PMCID: PMC11031161 DOI: 10.55730/1300-0144.5766] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/23/2023] [Revised: 02/15/2024] [Accepted: 01/05/2024] [Indexed: 05/31/2024] Open
Abstract
Background/aim Intestinal neomucosa formation is a technique defined for the treatment of short bowel syndrome. This study evaluates the effect of glutamine and omega-3 fatty acids on the growth of intestinal neomucosa on the colonic serosal surface has been evaluated. Materials and methods Thirty-two adult male Sprague-Dawley rats were randomly divided into 4 groups: sham, control, glutamine, and omega-3. Laparotomy was performed on all groups. For rats other than the sham group, a 1-cm full-thickness incision was made 4 cm proximal to the ileocecal valve, and colonic serosal surface was sutured as a serosal patch over these openings. By using the oral gavage technique, the glutamine group was ingested with 200 mg/kg/day of glutamine, and the omega-3 group was ingested with 100 mg/kg/day of omega-3 fatty acids. At the end of 14 days, the rats were euthanized, blood specimens were collected, and intestinal segments, including serosal patches, were excised. Results Transforming growth factor-beta was significantly lower in the glutamine group compared to the control group. Similarly, fibroblast growth factor-2 was significantly lower in the glutamine group compared to the sham group. Intestinal neomucosa formation was observed in 100% of rats in the glutamine group. In the control and omega-3 groups, intestinal neomucosa formation was observed in 57.1% and 60% of rats, respectively. The inflammatory response, granulation tissue formation, and fibroblastic activity were more severe in the rats of the glutamine and omega-3 groups. Conclusion The intestinal neomucosa formation is an experimental technique, and both glutamine and omega-3 fatty acids have the potential to positively affect inflammatory response, granulation tissue formation, and fibroblastic activity. Specifically, glutamine has a favorable effect on intestinal neomucosa formation.
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Affiliation(s)
- Mehmet KÖSTEK
- Department of General Surgery, University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul,
Turkiye
| | - Uygar DEMİR
- Department of General Surgery, University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul,
Turkiye
| | - Ramazan UÇAK
- Department of Pathology, University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul,
Turkiye
| | - Burak Yasin AVCI
- Department of Biochemistry, Amasya University, Sabuncuoğlu Şerefeddin Training and Research Hospital, Amasya,
Turkiye
| | - Aydın ÜNAL
- Department of Pediatric Surgery, University of Health Sciences, Bağcılar Training and Research Hospital, İstanbul,
Turkiye
| | - Osman Bilgin GÜLÇİÇEK
- Department of General Surgery, University of Health Sciences, Bağcılar Training and Research Hospital, İstanbul,
Turkiye
| | - Ozan ÇALIŞKAN
- Department of General Surgery, University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul,
Turkiye
| | - Bülent ÇİTGEZ
- Department of General Surgery, Üsküdar University, Memorial Hospital, İstanbul,
Turkiye
| | - Erdinç SERİN
- Department of Biochemistry, University of Health Sciences, Prof. Dr. Cemil Taşcıoğlu City Hospital, İstanbul,
Turkiye
| | - Sıtkı Gürkan YETKİN
- Department of General Surgery, University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul,
Turkiye
| | - Mehmet MİHMANLI
- Department of General Surgery, University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul,
Turkiye
| | - Mehmet ULUDAĞ
- Department of General Surgery, University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul,
Turkiye
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Yang GT, Zhao HY, Kong Y, Sun NN, Dong AQ. Study of the effects of nesfatin-1 on gastric function in obese rats. World J Gastroenterol 2017; 23:2940-2947. [PMID: 28522911 PMCID: PMC5413788 DOI: 10.3748/wjg.v23.i16.2940] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/01/2016] [Revised: 02/15/2017] [Accepted: 03/02/2017] [Indexed: 02/06/2023] Open
Abstract
AIM To investigate the effects of nesfatin-1 on gastric function in obese rats.
METHODS The obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats were determined after treatment with different drug concentrations of nesfatin-1 and administration routes. Based on this, the expression of H+/K+-ATPase was measured using RT-PCR and western blot to preliminarily explore the mechanism of gastric acid secretion changes.
RESULTS Body weight, body length, and Lee’s index of the rats significantly increased in the high-fat diet-induced obese rat model. Two hours after lateral intracerebroventricular injection of nesfatin-1, the gastric emptying rate and gastric acid secretory capacity of rats decreased. Four hours after injection, both were restored to normal levels. In addition, the expression of H+/K+-ATPase decreased and moved in line with changes in gastric acid secretory capacity. This in vivo experiment revealed that intracerebroventricular injection of nesfatin-1, rather than intravenous injection, could suppress gastric function in obese rats. Moreover, its effect on the gastric emptying and gastric acid secretory capacity of rats is dose-dependent within a certain period of time.
CONCLUSION Through this research, we provide a theoretical basis for further studies on nesfatin-1, a potential anti-obesity drug.
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