Lin J, Yan GT, Xue H, Hao XH, Zhang K, Wang LH. Role of leptin in hepatic ischemia/reperfusion-induced intestinal injury of rats.
Shijie Huaren Xiaohua Zazhi 2008;
16:3485-3491. [DOI:
10.11569/wcjd.v16.i31.3485]
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Abstract
AIM: To explore the changes of leptin in intestinal tract following hepatic ischemia/reperfusion (H-I/R), to investigate the association between these changes and H-I/R-induced intestinal injury, and to find out the role of leptin in H-I/R-induced intestinal injury.
METHODS: A 70% H-I/R model of rats was established, forming 5 groups including sham-operation and injury ones based on different reperfusion time. Enzyme-colorimetry was used to detect serum diamine oxidase activity after injury, hematoxylin-eosin staining and immunohistochemistry were applied to investigate pathological variations and leptin protein expressions in duodenum after injury, respectively, while reverse transcriptase-PCR was used to detect leptin mRNA expressions in duodenum after injury.
RESULTS: Compared with sham-operation group after injury, the four reperfusion groups showed no significant difference in serum diamine oxidase activity, but serum diamine oxidase level was significantly higher in 60 min ischemia/60 min reperfusion (I60'R60') group than in I60'R360' group (P = 0.0077). Pathological investigation suggested that duodenal impairments at the early phase of H-I/R were more serious, while the impairments at the later phase lessened gradually. Compared with leptin protein expression in duodenum of sham-operation group after injury, that of I60'R240' and I60'R360' groups increased significantly (0.126503 ± 0.005873, 0.129458 ± 0.003755 vs 0.079269 ± 0.001995, both P < 0.01), and the levels of reperfusion groups decreased in such order as I60'R360', I60'R240', I60'R60' and I60'R150' groups. Compared with leptin mRNA expression in duodenum of sham-operation group after injury, that of I60'R150' group decreased significantly (0.944 ± 0.033 vs 1.022 ± 0.011, P = 0.049), and it was significantly lower than the level of I60'R360' group.
CONCLUSION: The expression changes of leptin in intestinal tract after H-I/R are closely associated with intestinal injury, suggesting that leptin may be a protective factor of resisting H-I/R-induced intestinal injury.
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