Human exposure to microplastics (MPs) is widespread, attracting significant attention from both the public and the scientific community. Although several direct and indirect exposure pathways have been investigated, the extent of MP exposure from disposable medical devices remains poorly understood and warrants further research.

This work indicates that many MPs (10-30 μm) were released during the simulated use of disposable medical devices. Two common medical devices-disposable infusion tubes and blood needles-were selected as the research subjects. Analysis utilizing laser direct infrared (LDIR) revealed that plastic released from infusion tubes primarily consisted of polyamide (PA), polyvinyl chloride (PVC), and polyethene terephthalate (PET), with an average total number (ATN) of 11.8 particles/mL. MPs released from blood collection needles mainly consisted of polyurethane (PU) and PET, with an ATN of 82.7 particles/mL. For a 0.9 % normal saline, the ATN released from the infusion tubes during the stimulating infusion scenario at room temperature (4 h) was approximately 16 particles/mL, primarily consisting of PA, PVC, and PET. Additionally, the release of MPs increased with rising temperatures. Under the same conditions, ATN release from the blood collection needles was approximately 84.4 particles/mL, mainly from PA, PVC, and PU.

This implies that MPs can enter the bloodstream directly through infusion tubes and blood collection needles, highlighting the need for greater attention to the risk of patient exposure.

The surge in plastic production has spurred a global crisis as plastic pollution intensifies, with microplastics and nanoplastics emerging as notable environmental threats. Due to their miniature size, these particles are ubiquitous across ecosystems and pose severe hazards as they are ingested and bioaccumulate within organisms. Although global plastic production has reached an alarming 400.3 MTs, recycling efforts remain limited, with only 18.5 MTs being recycled. Currently, out of the total plastic waste, 49.6 % is converted into energy, 27 % is recycled, and 23.5 % is recovered as material, indicating a need for better waste management practices to combat the escalating pollution levels. Research studies on micro-nanoplastics have primarily concentrated on their environmental presence and laboratory-based toxicity studies. This review critically examines the sources and detection methods for micro-nanoplastics, emphasising their toxicological effects and ecological impacts. Organisms like zebrafish and rats serve as key models for studying these particle's bioaccumulative potential, showcasing adverse effects that extend to DNA damage, oxidative stress, and cellular apoptosis. Studies reveal that micro-nanoplastics can permeate biological barriers, including the blood-brain barrier, neurological imbalance, cardiac, respiratory, and dermatological disorders. These health risks, particularly relevant for humans, underscore the urgency for broader, real-world studies beyond controlled laboratory conditions. Additionally, the review discusses innovative energy-harvesting technologies as sustainable alternatives for plastic waste utilisation, particularly valuable for energy-deficient regions. These strategies aim to simultaneously address energy demands and mitigate plastic waste. This approach aligns with global sustainability goals, providing a promising avenue for both pollution reduction and energy generation. The review calls for further research to enhance detection techniques, assess long-term environmental impacts, and explore sustainable solutions that integrate energy recovery with pollution mitigation, especially in regions most affected by both energy shortages and increased plastic waste.

The adverse effects of plastics on the environment, wildlife, and human health have been extensively studied, yet their production remains unavoidable due to the lack of viable alternatives. Environmental fragmentation of larger plastic particles generates microplastics (MPs, 0.1-5000 μm) and nanoplastics (NPs, 1-100 nm), which can enter the bloodstream through inhalation or ingestion. This review examines whether MPs and NPs influence blood pressure. To address this question, relevant studies were analyzed based on predefined criteria. Due to anatomical barriers and microcirculatory dynamics, only NPs and small MPs are expected to enter the bloodstream under physiological conditions, although pathological states may alter this. In vitro research indicates that MPs and NPs negatively affect erythrocytes and endothelial cells, while rodent models suggest potential cardiovascular effects. Plastic particles and fibers have been detected in human blood, thrombi, atherosclerotic plaques, and various tissues. However, validated data on plastic particle-related blood pressure changes remain lacking. Despite limitations in their applicability to human physiology, preclinical models suggest that MPs and NPs circulate in the bloodstream, interact with blood cells, and contribute to vascular damage. Mechanisms such as endothelial injury, platelet activation, inflammation, and MPs/NPs accumulation in atherosclerotic plaques may contribute to blood pressure elevation but are unlikely to be the exclusive cause of hypertension. Further research is needed to clarify the role of plastic particles in blood pressure regulation. Standardized detection methods, real-world scenario-related models, and targeted human studies are essential to assessing cardiovascular risks associated with MP and NP exposure.

Microplastic and microfibre pollution is a global concern, however, karst areas remain understudied. Because of their properties, these anthropogenic microparticles are particularly hazardous, and easily transportable, reaching also remote areas. The underground world, called also dark continent, is a treasure of information, and remained the last frontier of terrestrial exploration: many parts of the underground world have not yet been accessed. In the hypogeal environments, pollution is closely linked to the connections between surface and subterranean habitats, the hydrodynamics of the aquifer, geology, and local environmental conditions. This study aims to investigate, for the first time, the presence of microplastics and microfibres in unexplored caves, revealing how human activity could indirectly impact even the uncontaminated environments of the dark continent. Together with speleologists, we collected and investigated sediment samples from unexplored caves of the Abruzzo Region, Italy. Examined anthropogenic microparticles were counted and characterized by composition, size, shape, fluorescence, and colour, via microscopy and spectroscopy. Microplastic concentrations resulted low or absent, moreover, natural and regenerated microfibres ones were higher. Fibre-shape was the most common. Most of the microparticles were clear and fluorescent under UV light. Pollution sources in this area likely include atmospheric deposition, nearby human activities, roads, and garbage. These results highlight anthropogenic microparticle pollution exists in unexplored karst caves, which could impact subterranean habitats, species, and water resources. Given the link between surface and underground karst environments, more monitoring and protection are needed. This work encourages speleologists to collect samples during explorations too, as these rarely studied environments offer crucial insights into karst systems, potential threats, and conservation needs. Future long-term studies will clarify pollutant sources, transport, and effects on ecosystems.

The widespread distribution of microplastics in the environment has raised concerns about their potential implications for human health. Microplastics accumulate in animals and humans, but the risks associated with these pollutants are not fully understood. This study aimed to investigate the effects of polystyrene microplastics on the male reproductive system. The 0.1 μm polystyrene (PS) could accumulate in the testicular tissue and spermatogonia GC-1, while 1 μm PS was not easy to enter and accumulate in the testicular tissue and cells. Mice continuously exposed for 3-months to 0.1 μm PS demonstrated lower fertility and inhibited spermatogonium differentiation compared to control mice. The 0.1 μm PS were dispersed throughout the seminiferous tubule of the testis. Metabolic reprogramming was found to be involved in these processes. Histone methylation and autophagy-related pathways showed significant differences following PS treatment in testis tissue and GC-1 cells. Our findings suggest that chronic exposure to 0.1 μm PS inhibited spermatogenic cell differentiation and impaired fertility in male mice. We propose that abnormal epigenetic modifications in 0.1 μm PS exposed mice contributed to the dysregulation of glycolytic enzymes, and that the impaired autophagic pathway exacerbated the accumulation of glycolytic enzymes further. Glycolysis plays a critical role in the regulation of spermatogenic cell differentiation, and its regulation partially alleviated the impairments associated with PS exposure. In conclusion, our findings suggest that chronic exposure to nanoplastics PS inhibited spermatogenic cell differentiation and impaired fertility in male mice via disrupted epigenetic modification and metabolic dysregulation.

This study presents a novel smartphone-based, machine-learning-assisted multispectral classification method for identifying airborne micro- and nanoplastics (MNPs). Instead of commercial polymeric microspheres, coffee grinder-based cryogrinding generated nonuniform MNPs from real-world plastic products with highly irregular shapes and heterogenous size distributions. The low-cost handheld device comprises a smartphone, a spectral mask array made from plastic color films, and a discrete multiplexed illumination device. A stack of images was captured across multiple wavelength ranges, and the RGB ratios were extracted without using morphological information. An XGBoost model was trained on two datasets: dry and wet MNP samples passively collected on a glass slide, simulating two types of airborne MNPs. The model successfully distinguished plastics from clay with 89-99 % accuracy and classified six plastic types with 79-87 % accuracy for dry and wet MNPs. This method offers a promising toolkit for airborne MNP monitoring.

MICRO: and nanoplastics (MNPs) are emerging environmental pollutants that pose a significant threat to human health, with traces found in cardiac tissues. While previous studies have indicated that MNPs can cantribute to cardiac dysfunction, there is limited systematic investigation into how MNPs exposure affects various organelles. This study focuses on polyvinyl chloride nanoparticles (PVC NPs), one of the most common and persistent plastic pollutants in the environment. Our findings reveal that PVC NPs engage in organelle-specific interactions, predominantly accumulating in the lysosomes and mitochondria of cardiomyocytes. This targeted accumulation results in substantial disruptions to lysosomal autophagic flux and mitochondrial energy metabolism. These results offer new insights into the organelle-specific mechanisms behind PVC NP-induced cardiotoxicity, highlighting the distinct risks associated with this widespread environmental contaminant.

Microplastics (MPs) are widely distributed as a global pollutant, with dynamic patterns driven by horizontal diffusion and vertical mixing in marine ecosystems across different hydrological seasons and regions. This study employed MATLAB for image processing and interactive operations to extract data from existing studies on the Chinese Marginal Seas conducted from 2016 to 2022, focusing on the distribution, sources, and transport processes of MPs. The results revealed that the Bohai Sea exhibited the highest pollution levels during both the rainy (9328.30 particles/m3) and dry (8665.80 particles/m3) seasons. The Yangtze River Estuary, the Pearl River Estuary, and the Bohai Bay are three hotspot regions that may significantly contribute to the enrichment of MPs in surrounding seas. The distance-similarity decay relationship indicated stronger correlations in the South China Sea and Bohai Sea during the rainy season, and in the East China Sea during the dry season. Derived from the analysis using the Positive Matrix Factorization model, it was found that, apart from the South China Sea where PE and PAN proportions were higher, PE and PP dominated the MP composition. Highly polluting and difficult-to-degrade industrial manufacturing and plastic packaging were identified as the primary sources, while the Bohai Sea and Yellow Sea posed higher transport risks, may serve as sources of pollution to the surrounding regions.

Nanoplastics are widely present in the environment. Exposure to environmental pollutants during pregnancy can have adverse effects on fetal development and health. Establishing a link between nanoplastics and Bronchopulmonary Dysplasia (BPD) requires further investigation. In this study, we examined the impact of prenatal exposure to 80 nm polystyrene nanoparticles (PS-NPs) on offspring lung development, taking into account potential gender-specific effects. Pregnant female mice were exposed to PS-NPs through oropharyngeal aspiration, and critical data on lung development were collected at postnatal days 1, 7, and 21. We found that exposure to PS-NPs reduced birth weight in female offspring and significantly increased lung weight in both male and female offspring by PND 21. Maternal exposure led to a reduction in alveolar numbers across offspring, with distinct underlying mechanisms observed between sexes. In female offspring, the reduction in alveolar numbers was linked to disrupted surfactant protein expression, significant inflammation, and increased apoptosis and fibrosis. In male offspring, impaired angiogenesis was the primary factor contributing to the increased risk of BPD. The impact on alveolar development was substantial in both genders. This study underscores the gender-specific impacts of prenatal nanoplastic exposure on lung development and offers new evidence and direction for future research on the cross-generational respiratory toxicity of PS-NPs.

The association between microplastics (MPs) exposure and cardiovascular disease is largely unknown. It is still unclear what effects MPs exposure have on blood pressure and how it affects the heart. As MPs age, their surfaces undergo modifications that may alter how the MPs interact with cells, which may affect the extent of their toxic effects. Here, we used three different surface functional-group polystyrene microplastics (PS-MPs), and exposed 5-week-old SD rats to them over 42 days. Compared with the control group, the mean blood pressure of the MPs exposed rats increased by 22-40%. Exposure to PS-MPs caused oxidative damage to the heart, and induced cardiomyocyte hypertrophy. More interestingly, MPs modified by functional groups induced enhanced adverse effects than unmodified PS-MPs, with amino-modified PS-MPs exhibiting more significant blood pressure elevation and myocardial hypertrophy. Proteomic analysis of cardiac differential proteins focused on factor XII activation, negative regulation of proteolysis, collectively pointed to the downregulation of kininogen. We demonstrated that MPs exposure induced ERK activation, the down-regulation of bradykinin, and inhibition of the downstream nitric oxide signaling pathway. This study demonstrates the different effects of MPs with different functional groups on blood pressure elevation and myocardial hypertrophy, and sheds light on the mechanisms responsible for microplastic-induced cardiovascular toxicity.

There is a growing consensus on addressing the global plastic pollution problem by advocating for bioplastics. While starch-based plastics are prevalent, the potential health implications of starch-based microplastics (SMPs) remain largely unexplored. This is particularly concerning given their potential for accidental ingestion and subsequent interference with blood glucose metabolism. Our research provides the first investigation into the distribution and adverse effects of long-term exposure to environmentally relevant doses of SMPs in female mice, approximately 14-81 particles per mouse per day. After three months of exposure, SMPs were found to infiltrate the liver, intestine, and ovarian tissues, causing microstructural lesions. Exposure to SMPs also resulted in elevated blood glucose levels, increased hepatic oxidative stress, and disrupted lipid metabolism. A multiomics analysis further uncovered abnormalities in gene expression and microbiota, as well as enriched pathways related to insulin regulation and circadian rhythms in the exposed mice. Our results indicate that prolonged exposure to environmentally relevant doses of SMPs can have widespread health effects in mice, potentially disrupting circadian rhythms by inducing insulin resistance. This suggests that the safety of bioplastics requires further evaluation before their large-scale application in food packages.

Residual stress is an intrinsic property of semicrystalline plastics such as polypropylene and polyethylene. However, there is no fundamental understanding of the role intrinsic residual stress plays in the generation of plastic pollutants that threaten the environment and human health. Here, we show that the processing-induced compressive residual stress typically found in polypropylene and polyethylene plastics forces internal nano and microscale segregation of low molecular weight (MW) amorphous polymer droplets onto the plastic's surface. Squeeze flow simulations reveal this stress-driven volumetric flow is consistent with that of a Bingham plastic material, with a temperature-dependent threshold yield stress. We confirm that flow is thermally activated and stress dependent, with a reduced energy barrier at higher compressive stresses. Transfer of surface segregated droplets into water generates amorphous polymer micropollutants (APMPs) that are denatured, with structure and composition different from that of traditional polycrystalline microplastics. Studies with water-containing plastic bottles show that the highly compressed bottle neck and mouth regions are predominantly responsible for the release of APMPs. Our findings reveal a stress-induced mechanism of plastic degradation and underscore the need to modify current plastic processing technologies to reduce residual stress levels and suppress phase separation of low MW APMPs in plastics.

Recently, the presence of microplastics (MPs) in common foods such as salt and beverages has been widely reported Microplastics (MPs) have been widely reported in common foods, including salt and beverages. MPs spread through the food chain and are eventually ingested into the human body through the diet. They have been found to accumulate in human feces, blood, and liver tissues, raising concerns about the effects of continuous intake of foods containing MP on the body. We examined whether rats could rapidly excrete polyethylene MPs (average particle size of 200 μm) when the MPs and were mixed with non-digestive dietary materials in their feed (indigestible dextrin, lactosucrose, chitosan, and eggshell membrane proteins). The group that ingested chitosan showed significant changes, including increased fecal weight, increased MP excretion rate, and decreased intestinal MP retention rate. The MP excretion rates in feces 0-144 h after ingestion were 83.7% ± 3.8% in the control group and 115.6% ± 4.5% in the chitosan group. These findings indicate that chitosan effectively promotes the expulsion of polyethylene MPs. The addition of chitosan to food may reduce the potential harm caused by MPa to a variety of organisms, including humans.

With the increasing prevalence of plastic pollution, including microplastics (MPs, particles <5 mm), the pursuit of safer and more sustainable alternatives gains increasing traction. While a substantial portion of MPs in the environment arises from the degradation of plastic litter and the wear of polymer-containing materials (secondary MPs), deliberate incorporation of MPs in certain products (primary MPs) also represents a considerable source, and targeted measures can be implemented to minimize human exposure and environmental releases. Improved policies for managing macroplastic waste help mitigate secondary MPs, but addressing primary MPs requires distinct strategies. Globally, various approaches, such as bans or restrictions on primary MPs, have been proposed, including the recent EU regulation under REACH, which groups intentionally added MPs together based on their diverse uses and properties. However, applying the Essential Use Concept (EUC) provides a more refined regulatory approach; balancing environmental health, technical feasibility, and innovation. This perspective explores the potential, challenges, and limitations of implementing the EUC for primary MPs. By examining four use cases─controlled-release medicines, agricultural seed coatings, personal care products, and artificial turf infill─we highlight how the EUC can prioritize essential and beneficial applications while phasing out nonessential uses.

The impact of nanoplastics (NPs) and microplastics (MPs) on ecosystems and human health has recently emerged as a significant challenge within the United Nations Agenda 2030, drawing global attention. This paper provides a critical analysis of the influence of plastic particles on plants and soils, with the majority of data collected from recent studies, primarily over the past five years. The absorption and translocation mechanisms of NPs/MPs in plants are first described, followed by an explanation of their effects-especially particles like PE, PS, PVC, PLA, and PES, as well as those contaminated with heavy metals-on plant growth, physiology, germination, oxidative stress, and nutrient uptake. The study also links the characteristics of plastics (size, shape, concentration, type, degradability) to changes in the physical, chemical, and microbial properties of soils. Various mitigation strategies, including physical, chemical, and biological processes, are explored to understand how they address these changes. However, further research, including both laboratory and field investigations, is urgently needed to address knowledge gaps, particularly regarding the long-term effects of MPs, their underlying mechanisms, ecotoxicological impacts, and the complex interactions between MPs and soil properties. This research is crucial for advancing sustainability from various perspectives and should contribute significantly toward achieving sustainable development goals (SDGs).

Growing evidence shows that breathing microplastics (MPs)-polluted air increases the risk of pulmonary health effects. However, a complete understanding of how inhaled MPs distribute within the human respiratory tract (HRT) remains insufficient. This study developed a physiologically-based kinetic HRT model to evaluate the deposition and clearance of MPs over time and at varying concentrations based on their aerodynamic diameter (AD). We quantified the contributions of AD-specific MPs to inhalation exposure trends using literature-based atmospheric MP pollution data from 2015 to 2022. Exposure assessments were conducted in data-rich settings, including megacities, urban-rural, and age-specific populations. Our analysis revealed that all suspended MPs had ADs less than 70 μm, with fragments, fibers, and spheres in decreasing order of prevalence. Modeling results demonstrated a pronounced variation (∼1010 magnitudes) in internal MP burdens across airway regions during long-term exposure. On average, inhaled MPs larger than 40 μm accumulated exclusively in extrathoracic and bronchi regions, whereas MPs with ADs of 0.1-5 μm were the primary contributors to internal burdens. We identified nasal airflow rate as the most sensitive factor influencing internal burdens of MPs larger than 1 μm. Furthermore, our findings showed that infants, children, and the elderly were more vulnerable to short-term exposure, whereas adolescents and adults were of greater concern with long-term exposure. These insights provide valuable guidance for policy decisions on targeting interventions to at-risk regions or susceptible populations.

The accumulation of plastic waste in the environment has raised widespread concern about the impact of microplastics (MPs) on human and environmental health, particularly regarding aged MPs. This study investigated the effects of subchronic dietary intake on pristine and aged polyethylene microplastics (PE-MPs) in C57BL/6J mice. Results revealed that both pristine and aged PE-MPs, at doses of 0.01 and 1 mg/day, induced plasma metabolic changes primarily associated with lipid metabolism and digestive processes. These alterations were reflected in the expression changes of proteins involved in unsaturated fatty acid pathways in the liver as well as a reduction in beneficial gut microbiota. Key contributors in the toxicity of aged PE-MPs included ATP-binding cassette transporters, gut bacteria alterations (notably Lactobacillus, Akkermansia, Parasutterella, and Turicibacter), and significantly altered proteins related to fatty acid elongation, such as acyl-CoA thioesterase enzyme family and elongation of very long chain fatty acid protein 5. These disruptions exacerbated lipid metabolism disorders, potentially contributing to metabolic diseases. Additionally, decreased levels of glutathione S-transferase A proteins, along with reduced hepatic glutathione and increased reactive oxygen species in both the small intestine and liver, suggested that aged PE-MPs aggravated hepatic and intestinal damage through oxidative stress. These findings indicated that aged PE-MPs caused more severe hepatic dysfunction and gut microbiota disruption. This effect was likely mediated by the transfer of fatty acids and signaling molecules through the gut-liver axis, ultimately leading to hepatic lipid metabolism disorders and oxidative stress.

The interfacial adsorption, aggregation and deposition processes of nanoplastics (NPs) on clay mineral surfaces critically regulate their environmental mobility, transformation pathways, and ecotoxicological risks in aquatic ecosystems. A quantitative understanding of the nanoscale interfacial processes is essential. This study employs molecular dynamics (MD) simulations and density functional theory (DFT) calculations to elucidate the aggregation and deposition mechanisms of three types of NPs in their pristine and aged states in the nanopore solution of montmorillonite (Mt). In the wet environment, NPs tend to form aggregates in the nanopore and migrate in solution, increasing environmental risk, while in the dry environment, NPs are more likely to deposit on the basal surface to form larger aggregates, consequently reducing their mobility. Results show hydrophobic interactions play as the primary driving force for the aggregation of pristine NPs, and both hydrophilic and hydrophobic interactions contribute to the aggregation of aged NPs. Aged NPs exhibit stronger binding affinity to Mt through mechanism such as Ca²⁺ bridging and hydrogen bonding, compared to their pristine counterparts. DFT calculations further reveal the formation of hydrogen bonds between the hydroxyl groups of aged NPs and the tetrahedral oxygen atoms in Mt. Through atomic-level characterization of interfacial processes, this work establishes a predictive framework for NP environmental behavior by resolving migration dynamics and retention processes in nanopore water.

As emerging pollutants, antibiotic resistance genes (ARGs) and microplastics threaten the environment and human health. Gut microbiota is a hotspot for ARG emergence and spread. However, effects of microplastic exposure on the emergence and spread of gut microbial ARGs are unclear. Therefore, metagenomics was used to characterize polystyrene microplastics (PS)-induced ARG alterations in rat gut microbiota and their health risks, and to identify key ARG hosts and pathways as intervention targets. We found that PS exposure not only induced selective accumulation of glycopeptide and aminoglycoside ARGs, but also promoted mobility risks of glycopeptide and macrolide-lincosamide-streptogramin ARGs in gut microbiota. Metagenomic reassembly identified microbes belonging to Firmicutes (particularly order Clostridiales, such as speices Lachnospiraceae bacterium 3-1 and MD335) as major ARG hosts. Meanwhile, genera Enterococcus, Clostridioides and Streptococcus were main ARG hosts among human pathogens. Furthermore, glycopeptide and aminoglycoside ARGs were highly correlated with VanS/VanR signaling and its regulatory pathways of vancomycin resistance and peptidoglycan metabolism, amino sugar and nucleotide sugar metabolism, and CpxR signaling and its regulatory remodeling of cell envelope peptidoglycans and proteins in gut microbiota upon PS exposure. This study provides novel insights and intervention targets involved in PS-induced changes in gut microbial ARGs and their health risks.

Four common bivalves, including white clam (Meretrix lusoria), lined clam (Paratapes undulatus), oysters (Crassostrea gigas), and green mussels (Perna viridi), which are commonly consumed in Central Vietnam, were collected from Tam Giang-Cau Hai and O Loan Coastal Lagoons. The samples were investigated for the presence of microplastics (MPs) in their tissues. The average number of MPs determined in white clams, lined clams, oysters, and green mussels in Central Vietnam varies from 0.3 to 0.9 per g-ww and from 0.9 to 5.6 per individual. Fibers, fragments, and pellets were found with various proportions concerning. Fibers were the most common shape, making up 36-74 % of the total microplastics, followed by fragments accounting for 16-47 %. The most prevalent colors were white-transparent and black-grey, comprising 49-81 % of the MPs. Regarding the microplastics found in the bivalve tissues, 78-80 % were <500 μm. Given chemical analysis, rayon accounted for 38 % of the microplastics discovered in bivalve tissues; closely PET (13 %), PA (10 %), and PP (10 %) were followed. This study offers valuable insights into the microplastic contamination concerned by bivalve consumption in Thua Thien Hue and Phu Yen, Central Vietnam; the results estimate the annual intakes are between 5000 and 10,000 particles per person. Unprecedentedly addressed in the literature, these findings contribute to a better understanding of microplastic pollution in Vietnam. The results altogether provide solid shreds of evidence for the MP contamination in Vietnam-based seafood, thus encouraging further attempts for plausible socio-economical regulations and raising public awareness on the issue.

This paper examines microplastic hotspots and their drastic effects on human health and the environment pointing out microplastic pollution as one of the biggest global issues. Besides, it analyses the key sources including industrial effluent discharge, littered plastic wastes, and deterioration of synthetic products together with pathways and routes of exposure. The review also focuses on microplastic contamination in food systems such as meat, plant-based products, dairy, and seafood, detailing their entry into the food chain via soil, water, and air. On the other hand, this work also focuses on human health issues including cellular absorption, and bioaccumulation, which results in tissue oxidative stress, inflammation, hormonal imbalance and adverse long-term effects, including carcinogenicity and organ toxicity. The ultimate effects of microplastic pollution on the condition of the soil, water, and fauna and flora of the ecosystem, highlighting on the need for the prevention measures, were also addressed. This paper seeks to critically ascertain the problems posed by microplastics, including their slow biodegradation limit, the absence of proper regulations, and lack of a universally accepted standard. It also highlights that microplastic pollution requires interdisciplinary analyses, future studies, and high standards-compliant policies and regulations. This work raises the alarm for a collective international effort to protect the public health, food, and the earth.

Atmospheric microplastics (AMPs) transport and deposition in urban areas contribute to microplastics pollution. The present study investigates AMPs deposition, characteristics, potential sources, and the influence of meteorological factors in a central Indian city. AMPs were collected over three land-use types, viz. institutional, commercial, and industrial areas, during four seasons: summer, monsoon, autumn, and winter. The deposition flux of microplastics ranged from 212.53 ± 52.32 to 543.25 ± 71.23 particles/m2/day. The AMPs were predominantly fibres (87.84 %), followed by films (5.43 %), with particle size <1000 μm contributed 43.67 %. The predominant polymer types identified were polyethylene terephthalate (PET, 37.39 %), nylon (20.49 %), and polypropylene (PP, 10.27 %). Higher deposition fluxes were recorded in summer, with 491.06 ± 73.37 particles/m2/day. Correlation analysis revealed a negative correlation between rainfall and AMPs deposition, suggesting a potential cleaning role of rainfall. The estimated annual deposition flux of AMPs in Nagpur city was 3.22 × 1013 particles. Higher AMPs deposition was attributed to plastic waste littering, industrial emissions, and textiles. The estimated mean annual inhalation exposures of AMPs of size 50-250 μm for children and adults were 7375.84 ± 1312.89 and 3738.17 ± 665.39 MPs/kg-bw/year, respectively. The findings of this study contribute to understanding the fate of AMPs and its implications for human exposure. The findings underscore the importance of reducing and managing plastic waste.

Plastics are everywhere. It is widely recognized that they represent a global problem, the extent of which is yet to be defined. Humans are broadly exposed to plastics, whose effects and consequences are poorly characterized so far. The main route of exposure is via alimentary and respiratory intake. Plastics pollutions may come from both: water and food contamination itself, and their packaging. The smaller sizes (i.e. microplastics <150 µm - MPs) are considered to be the most pervasive of living organisms and, therefore, potentially the most harmful. As humans occupy one of the apex positions of the food chain, we are exposed to bioaccumulation and biomagnification effects of MPs. In fact, MPs are commonly found in human stools and blood. However, there are no data available yet on their ability to accumulate and to produce detrimental consequences on biological systems. Even though the effects of plastics pollution are poorly studied in mammals, including humans, they appear to have inflammatory effects, which is rather concerning as many etiologies of disease are based on a pro-inflammatory status.

Agricultural plastic film mulch (PFM) covers ca. 50 million hectares of the Earth's surface and has revolutionized agriculture, particularly in arid and semi-arid regions, by improving crop yields, water use efficiency, farmer incomes and feeding an extra 85 million people in China alone. However, concerns are growing about the impact of PFM-derived microplastics (MP) on soil quality, the food chain, and the environment. Here we show that current research on the effects of MP in agricultural soils is limited by inconsistent methodologies and unrealistic experimental concentrations, leading to major uncertainty in assessing the true risks associated with PFM use. Furthermore, we highlight the need for standardized protocols, experiments using realistic MP concentrations, and a better understanding of the relative contribution of PFM to MP pollution to develop informed policies. Furthermore, while biodegradable alternatives show promise, their significantly higher costs (2-3 times that of conventional LDPE PFM) and variable performance across different agricultural environments present economic and practical challenges that must be addressed through targeted policy incentives and continued technological innovation. Our findings suggest that while further research is conducted, managing PFM to reduce environmental impact, rather than imposing ill-informed bans on plastic use, is crucial to balance food security and sustainable development goals. Exploring "zero-leakage" instead of "zero-use" approaches to PFM should be the primary aim to help mitigate potential risks while preserving the substantial benefits of this agricultural technology.

To investigate microplastics (MP) contamination in artificial tear (AT) products.

Five hyaluronic acid ATs (two multi-use and three disposable ATs) were used to gauge MP levels in three scenarios: 1) initial drop and remaining liquid after opening the lid upward; 2) remaining liquid after opening the lid downward and discarding two drops; and 3) remaining liquid after opening the lid downward and discarding half of it. Raman spectroscopy was used to identify the quantity, morphological characteristics, and composition of MPs. Scanning electron microscopy/energy dispersive spectroscopy was used to examine the surface traits and elements of MPs and ATs.

MPs were detected in 4 out of 5 ATs in the initial drops, containing 0.50 ± 0.65 particles/30 mL, whereas the remaining solution had 0.75 ± 0.72 particles/30 mL. After discarding two drops, 0.14 ± 0.35 particles/30 mL were present in the remaining solution. No MPs were detected after discarding half drops. Most MPs were transparent (95 %), irregular fragments (55 %) sized 10-20 μm (35 %), and made of polyethylene (95 %). If patients use the first drops of ATs four times a day for a year, individuals can be exposed to 730.0 particles. This exposure can be reduced to 204.4 particles by discarding the first two drops before use.

MPs are observed in commercially available ATs, and human eyes may be directly exposed to MPs through the use of ATs.

Plastic is a widely used material across various industries, including construction, packaging, healthcare, and automotive, among others. Global plastic production was estimated at 311 million tonnes in 2014 and is expected to double within two decades, continuing to rise towards 2050. As plastic pollution poses significant environmental and health risks, effective recycling and upcycling strategies are crucial for sustainable waste management. This paper explores the impact of plastic waste on public health and ecosystems, reviews chemical, mechanical, and biological recycling methods, and examines upcycling approaches. It also addresses key challenges such as limitations in chemical upcycling, scaling up carbonization, and inefficiencies in sorting and processing for mechanical recycling. Additionally, recent innovations-including enzymatic depolymerization for PET recycling, upcycling plastic waste into advanced carbon materials like graphene and carbon nanotubes, photochemical and photocatalytic upcycling, PVC recycling via Cl-transfer systems, and advancements in mechanical recycling for multi-layer plastics-are discussed to highlight emerging solutions in plastic waste management. By addressing these challenges and gaps, this paper provides valuable insights into advancing plastic waste management through innovative recycling and upcycling technologies, paving the way for more sustainable and environmentally friendly solutions to combat global plastic pollution.

The presence of microplastics in the human body and their potential health risks have drawn widespread attention in recent years. Microplastics have been detected in human blood, though their pathways of entry remain unclear. This study employed Raman spectroscopy and energy dispersive spectroscopy to evaluate the microplastic release characteristics of intravenous medical devices, aiming to investigate the influencing factors and the risk of microplastics entering the bloodstream. The results showed that microplastics were found in three widely-used medical devices, with abundances ranging from 0.44 to 2.00 items/n. Polyethylene, polypropylene (46.2 %), fragments (96.7 %), and white (86.8 %) were the predominant characteristics. Factors such as brand, specifications, and usage scenarios influence microplastic release, leading to differences in detection rates among different medical devices (0-100%). Repeated use significantly increases the risk of microplastic release (p < 0.05). Notably, built-in filtration membranes do not completely retain microplastics and may pose a risk of shedding fibers themselves. Using the exposure assessment model, the estimated microplastic release per person per year was 3.75 items for syringe, 6.22 items for infusion set, and 0.35 items for vein detained needle. Overall, although the amount of microplastics entering the human body through intravenous injection is significantly lower than that from dietary exposure and other pathways, the risk of direct entry into the bloodstream remains a concern. This research provides critical evidence for understanding the direct pathways and risks of microplastic exposure in human blood from plastic medical devices, offering significant scientific value for assessing exposure pathways and the safety of medical device use.

Plastic cutting boards are commonly used in food preparation, increasing human exposure to microplastics (MPs). However, the health implications are still not well understood.

The objective of this study was to assess the impacts of long-term exposure to MPs released from cutting boards on intestinal inflammation and gut microbiota.

MPs were incorporated into mouse diets by cutting the food on polypropylene (PP), polyethylene (PE), and willow wooden (WB) cutting boards, and the diets were fed to mice over periods of 4 and 12 wk. Serum levels of C-reactive protein (CRP), tumor necrosis factor-α (TNF-α ), interleukin-10 (IL-10), lipopolysaccharide (LPS, an endotoxin), and carcinoembryonic antigen (CEA), along with ileum and colon levels of interleukin-1β (IL-1 β ), TNF-α , malondialdehyde (MDA), superoxide dismutase (SOD), secretory immunoglobulin A (sIgA), and myosin light chain kinase (MLCK), were measured using mouse enzyme-linked immunosorbent assay (ELISA) kits. The mRNA expression of mucin 2 and intestinal tight junction proteins in mouse ileum and colon tissues was quantified using real-time quantitative reverse transcription polymerase chain reaction. Fecal microbiota, fecal metabolomics, and liver metabolomics were characterized.

PP and PE cutting boards released MPs, with concentrations reaching 1,088 ± 95.0 and 1,211 ± 322 μ g / g in diets, respectively, and displaying mean particle sizes of 10.4 ± 0.96 vs. 27.4 ± 1.45 μ m . Mice fed diets prepared on PP cutting boards for 12 wk exhibited significantly higher serum levels of LPS, CRP, TNF-α , IL-10, and CEA, as well as higher levels of IL-1β , TNF-α , MDA, SOD, and MLCK in the ileum and colon compared with mice fed diets prepared on WB cutting boards. These mice also showed lower relative expression of Occludin and Zonula occludens-1 in the ileum and colon. In contrast, mice exposed to diets prepared on PE cutting boards for 12 wk did not show evident inflammation; however, there was a significant decrease in the relative abundance of Firmicutes and an increase in Desulfobacterota compared with those fed diets prepared on WB cutting boards, and exposure to diets prepared on PE cutting boards over 12 wk also altered mouse fecal and liver metabolites compared with those fed diets prepared on WB cutting boards.

The findings suggest that MPs from PP cutting boards impair intestinal barrier function and induce inflammation, whereas those from PE cutting boards affect the gut microbiota, gut metabolism, and liver metabolism in the mouse model. These findings offer crucial insights into the safe use of plastic cutting boards. https://doi.org/10.1289/EHP15472.

The presence of micro- and nano-plastics (MNPLs) in the environment has increased significantly in the past decades. However, the direct impact of MNPL particles on human health remains unclear.

In this study, we utilized a modified extraction method with a previously reported staining technique to develop a novel approach for identifying individual plastics in mixtures of MNPLs of commercial and environmental origins to be able to investigate their impacts on human cell inflammation and cell death. Polypropylene (PP), polyethylene (PE), polystyrene (PS), and polyethylene terephthalate (PET) were the plastics analyzed. The plastic composition of the environmental MNPLs was characterized using multiple analytical techniques, including Fourier transform infrared spectroscopy, confocal imaging, scanning electron microscopy, and X-ray diffraction.

We found that both commercial and environmental MNPLs, especially PET, impose a strong inflammatory response on various human cells and tissues. At 1 mg/mL, they robustly stimulate inflammatory IL-1β and IL-6 secretion in a time-dependent manner. Importantly, we observed that the MNPLs induced variable inflammatory responses in cells depending on their plastic composition. Environmental samples rich in PET showed a strong dose-dependent response and induced IL-1β secretion at doses as low as 100 ng/mL. In addition, MNPLs can induce human cell death with or without obviously altering the cell morphology.

These findings are significant because they represent the first instance of authentic MNPLs being collected from ecological water samples for characterization and the first time the direct influences of commercial and environmental MNPLs have been compared in human cell studies. The methods developed in this study provide a foundation for future research to isolate MNPLs from the environment and explore their potential impacts on human health and disease development.

The food safety risks posed by exposure to polystyrene microplastics (PS-MPs) and bisphenol A (BPA) have become an issue worldwide. However, the toxic effects of PS-MPs and BPA coexposure on the mammalian liver remain elusive. In this study, we found that PS-MPs and BPA coexposure have synergistic toxic effects on AML12 cells and the mouse liver. Histopathological staining revealed excessive accumulation of the extracellular matrix in the coexposure liver. Co-exposure to PS-MPs and BPA downregulated Bmal1 and E-cad both in vitro and in vivo. Additionally, Bmal1-/- AML12 cells and liver-specific Bmal1-/- mice exhibited significantly reduced E-cad levels, with no significant reduction under PS-MPs and BPA coexposure. Notably, overexpression of BMAL1 and CLOCK significantly enhanced luciferase activity driven by the E-cad gene intron region (containing an E-box cis-element). These results demonstrated that coexposure to PS-MPs and BPA contributed to the development of liver fibrosis by inhibiting the BMAL1/E-cad signaling pathway.

The inevitable exposure of humans to micro/nanoplastics has become a pressing global environmental issue, with growing concerns regarding their impact on health. While the direct effects of micro/nanoplastics on human health remain largely unknown, increasing attention is being given to their potential role as carriers of environmental pollutants and organic substances. This study investigates the direct toxicity of 500 nm polystyrene nanoplastics (NPs) on human hepatocytes (HepG2) in vitro, both alone and in combination with cadmium (Cd), a hazardous heavy metal and a prevalent environmental pollutant. One-hour exposure to 100 µg/mL of NPs causes a significant increase in ROS production (+25% compared to control) but cell viability remains unaffected even at concentrations much higher than environmental levels. Interestingly, NPs significantly reduce Cd cytotoxicity at LC50 concentrations (cell viability compared to control: 55.4% for 50 µM Cd, 66.9% for 50 µM Cd + 10 µg/mL NPs, 68.4% for 50 µM Cd + 100 µg/mL NPs). Additionally, NPs do not alter the cellular lipid content after short-term exposure (24 h). However, when Cd and fatty acids are added to the medium, NPs appear to sequester fatty acids, reducing their availability and impairing their uptake by cells in a dose-dependent manner. We confirmed by Dynamic Light Scattering and Scanning Electron Microscopy the interaction between NPs, Cd and free fatty acids. Although polystyrene NPs exhibited minimal cytotoxicity in our experimental model, collectively our findings suggest that predicting the effects of cell exposure to NPs is extremely challenging, due to the potential interaction between NPs, environmental pollutants and specific components of the biological matrix.

Environmental pollutants significantly impact liver disease development, progression, and outcomes. This review examines the complex relationship between environmental exposures and liver pathology, from malignant conditions like hepatocellular carcinoma to steatotic and cholestatic liver diseases. Key environmental factors include air pollutants, volatile organic compounds, persistent organic pollutants, heavy metals, and per- and polyfluoroalkyl substances. These compounds can act through multiple mechanisms, including endocrine disruption, metabolic perturbation, oxidative stress, and direct hepatotoxicity. The impact of these exposures is often modified by factors such as sex, diet, and genetic predisposition. Recent research has revealed that even low-level exposures to certain chemicals can significantly affect liver health, particularly when combined with other risk factors. The emergence of exposomics as a research tool promises to enhance our understanding of how environmental factors influence liver disease. Importantly, exposure effects can vary by demographic and socioeconomic factors, highlighting environmental justice concerns. Implementation of this knowledge in clinical practice requires new diagnostic approaches, healthcare system adaptations, and increased awareness among medical professionals. In conclusion, this review provides a comprehensive examination of current evidence linking environmental exposures to liver disease and discusses implications for clinical practice and public health policy.

The increasing accumulation of plastics in the environment has raised concerns regarding their potential health hazards. Nanoplastics (NPs) can get transported across the placental barrier, resulting in detrimental effects on developing offspring. To date, the effects of maternal exposure to NPs during pregnancy on the cardiac toxicity in adult offspring have not been conclusively evaluated. Herein, the potential for cardiac injury in the progeny of adult mice that were gestationally exposed to 80 nm polystyrene NPs (PS-NPs) at different doses (0, 0.5, 1, and 5 µg µL-1) through oropharyngeal aspiration was investigated. Gestational exposure to PS-NPs resulted in cardiac fibrosis and cardiomyocyte apoptosis, and induced an increase in malondialdehyde (MDA) levels in adult offspring hearts, which were sex-specific and dose-dependent. The mRNA expression levels of estrogen receptor (ER)-related genes, such as Esr1, Esr2, and GPER1, were found to be significantly decreased on exposure to low-dose PS-NPs but elevated on exposure to high-dose PS-NPs in offspring hearts. Furthermore, the magnitude of this elevation in male offspring significantly exceeded compared to that of the female offspring. Additionally, the expression levels of Esr2 and GPER1 in male offspring that were gestationally exposed to high-dose PS-NPs were found to be higher than those observed in female offspring. The observed sex difference in cardiac fibrosis may be correlated with oxidative stress and changes in ER-related gene expression in the offspring's heart. Overall, our study demonstrated that gestational PS-NP exposure induces significant cardiac injury in adult offspring, providing crucial data on the transgenerational effects of PS-NP exposure in mice.

Microplastics (MPs) have been detected in all environmental spheres, from the Arctic to the deepest ocean trenches, and have also infiltrated the internal organs of the human body through ingestion, inhalation, and other exposure routes. While various commercial products have been identified as origin of MPs, leading to bans and awareness campaigns, their presence in medical treatments remains underexplored. This study investigates MPs in intravenous (IV) admixtures, which are stored in plastic containers before administration. The hypothesis suggests that prolonged storage may degrade container walls, leading to the release of MPs into the solutions. Analysis of 11 IV admixtures with the help of a stereomicroscope revealed a significant presence of fibre and fragment particles, with 99% of detected MPs measuring less than 100 µm. Polymers identified through a micro FTIR included polypropylene-polyethylene (PP-PE) copolymer, polypropylene (PP) homopolymer, polyvinylpyrrolidone (PVP), and polyurethane (PU). The abundance of MPs increased with storage duration, with older solutions exhibiting more surface roughness, indicating progressive degradation of plastic materials over time. These findings highlight an overlooked route of MP exposure, directly introducing these particles into the human body during medical treatments. Given the increasing use of IV therapies worldwide, further research is essential to assess the health risks posed by MPs in medical solutions. Regulatory measures should be considered to minimize contamination and ensure patient safety.

Micro- and nanoplastics (MNPs) are widespread in the environment and food, posing ingestion risks through various pathways. However, their transformation in human body fluids (SBFs), especially the formation of secondary nanoparticles (NPs), is not well understood due to inadequate quantification methods. This study proposed a robust method for quantifying eight common MNPs using pressurized liquid extraction (PLE) for pretreatment and pyrolysis gas chromatography-quadrupole time-of-flight mass spectrometry (Py-GC-QTOF-MS) for analysis. The method demonstrated high performance with recoveries over 90.9 % and a detection limit down to 0.01 mg/L. Most sample matrices did not interfere with MNP quantification, though poly(3-hydroxybutyrate) and polyethylene required background noise deduction. High recoveries in SBFs (>79.0 %) further confirmed the practicality of this method. Utilizing this method, it was found that only a few MPs were able to release secondary NPs within the simulated digestive system, with the maximum proportion of released NPs less than 2.1 %, suggesting a negligible health risk from secondary NPs. Besides, ester structure was found not to promote the formation of secondary NPs but did affect surface morphology and functional groups to a certain extent. We anticipate that this work will open opportunities for the health risk assessment of MNPs.

This article presents a simple and low-cost screening method based on optical microscopy and FT-IR spectroscopy for assessing microparticles found in rice. Five brands of rice packed in paper and foil bags from both the European Union (EU) and non-EU region were tested. Microparticles of various shapes have been found in the rice regardless of the packaging type and origin of the rice. The content of microparticles varies depending on the sample, from 2 to even 12 items per 1 g of rice. Overall, the abundance of microparticles is higher in the case of rice packed in foil bags. Not all identified microparticles are microplastics, but those that are microplastics cannot be directly linked to the composition of the rice package. For a cursory analysis aimed at distinguishing the infrared spectra of non-plastic microparticles (i.e. rice, paper or cellulose) from microplastics, it is sufficient only to analyse the absorption bands above 2800 cm-1.

The impact of polystyrene microplastics (PS-MPs) on the nervous system has been documented in the literature. Numerous studies have demonstrated that the activation of the epidermal growth factor receptor 4 (ErbB4) is crucial in neuronal injury and regeneration processes. This study investigated the role of targeted activation of ErbB4 receptor through a small molecule agonist, 4-bromo-1-hydroxy-2-naphthoic acid (C11H7BrO3, E4A), in mitigating PS-MPs-induced neuronal injury. The findings revealed that targeted activation of ErbB4 receptor significantly ameliorated cognitive behavioral deficits in mice exposed to PS-MPs. Furthermore, E4A treatment upregulated the expression of dedicator of cytokinesis 3 (DOCK3) and Sirtuin 3 (SIRT3) and mitigated mitochondrial and synaptic dysfunction within the hippocampus of PS-MPs-exposed mice. E4A also diminished the activation of the TLR4-NF-κB-NLRP3 signaling pathway, consequently reducing neuroinflammation. In vitro experiments demonstrated that E4A partially alleviated PS-MPs-induced hippocampal neuronal injury and its effects on microglial inflammation. In conclusion, the findings of this study indicate that targeted activation of ErbB4 receptor may mitigate neuronal damage and subsequent neuroinflammation, thereby alleviating hippocampal neuronal injury induced by PS-MPs exposure and ameliorating cognitive dysfunction. These results offer valuable insights for the development of potential therapeutic strategies.

Because of the ubiquity of microplastics (MPs) in the environment there are concerns regarding human exposure. In this study, MPs have been determined in three physiological fluids: urine, sputum and bronchoalveolar fluid (BALF); from 30 adult patients in Iran with respiratory conditions. A total of nine small (20-100 μm) and mainly green and red fibres of polyethylene, polypropylene and polystyrene construction were detected in urine samples of eight participants. By contrast, 358 MPs that were dominated by small, white and transparent fibres, but also included larger (100-500 μm) fibres and fragments and spherules of various sizes, were detected in sputum samples. Here, a broader range of polymers was identified but polyurethane was dominant. In BALF samples, 123 MPs were detected that included a higher proportion of larger fibres, along with fragments and spherules. The colour distribution of these MPs was similar to that of sputum samples but polymer distribution was closer to that of urine samples. These observations suggest that MPs that are inhaled and ingested might be fractionated differently though the body. Further research is required to elucidate how particles larger than theoretical limits (set by filtration mechanisms) are present in physiological fluids, what fractionation processes are present, and whether ingested or inhaled MPs are responsible for acute and chronic health impacts.

The pervasive presence of microplastics (MPs) in aquatic environments, coupled with their potential to act as vectors for toxic contaminants, raises significant concerns for human health. This study quantifies the health risks associated with the ingestion of microplastics and their co-contaminants in aquatic medium, considering both individual and interactive effects. The analysis encompasses four MP types (PP, PS, PET, PE) and prevalent contaminants including heavy metals (Cr, Cu, Ni, Pb), polycyclic aromatic hydrocarbons (PAHs, expressed as BaP equivalents), and plastic additives (DEHP, DBP, BPA)-to calculate individual Hazard Quotient (HQ), interaction-based Hazard Index (HIint), individual Incremental Lifetime Cancer Risk (ILCR), and interaction-based ILCR (ILCRint). The mean concentration of MPs in aqueous media was determined to be 2.19 mg/L (95 % CI), and Chronic Daily Intake (CDI) values were derived from particle counts converted to mass using polymer-specific densities. Reference Dose (RfD) values were calculated using the Weight of Evidence (WoE) approach, which integrates findings from rodent toxicity studies, identifying PP and PS as having low RfD values 25 × 10⁻⁴ mg/kg bw/day and 8 × 10⁻⁴ mg/kg bw/day, respectively. HQ-based toxicity rankings indicated the order of risk as PP > PS > PE > PET. Findings revealed a pronounced HIint of 18.646 × 10³ and 16.649 × 10⁶ at the 50th and 90th percentiles in children, underscoring significant synergistic effects from combined exposure to MPs and leached plastic additives. Co-contaminant scenarios further escalated health risks, with HI values reaching 52.236 in the presence of heavy metals and 53.141 with PAHs. The maximum allowable MP concentration, considering additive leaching, was estimated at 0.011 mg/L. This research highlights the need for firstly understanding the transformations of microplastic in the aquatic medium along with co-contaminants and framing regulatory measures and improved monitoring to protect human health from the growing threat of microplastic pollution. By integrating exposure modeling, dose-response assessment, and Monte Carlo simulations, the study delivers a robust framework for environmental health guidelines. It emphasizes the complex, multifaceted risks MPs pose and their associated contaminants, calling for innovative solutions to safeguard public health against this pervasive environmental challenge.

Plastics play a crucial role in modern life, but improper use and disposal have resulted in microplastics becoming widespread in the environment, raising significant concerns about both the environment and human health. Extensive research has explored the transformation mechanisms, bioaccumulation, ecological impacts, and health risks associated with microplastics. The present review first analyzes the migration, transformation, and degradation pathways of microplastics on a global scale, and then synthesizes current knowledge on the types, sources, and migration pathways of microplastics in soil, atmosphere, and aquatic environments, emphasizing transformation mechanisms like photo-aging and microbial degradation, and detailing their ecological and human health impacts. Additionally, this review examines gaps in current research and identifies critical areas needing further study, such as key control points in microplastic degradation processes and the mechanisms underlying health risks to populations. The aim is to provide a comprehensive reference for advancing microplastic pollution control, ecological protection efforts, and health risk assessment frameworks.

Concerns about the negative impacts of microplastics on human health are increasing in society, while exposure and risk assessments require high-quality, reliable data. Although quality assurance and -control (QA/QC) frameworks exist to evaluate the reliability of data for these purposes, manually assessing studies is too time-consuming and prone to inconsistencies due to semantic ambiguities and evaluator bias. The rapid growth of microplastic studies makes manually screening relevant data practically unfeasible. This study explores the potential of artificial intelligence (AI), specifically large language models (LLMs) such as OpenAI's ChatGPT and Google's Gemini, to streamline and standardize the QA/QC screening of data in microplastics research. We developed specific prompts based on previously published QA/QC criteria for the analysis of microplastics in drinking water and its sources, and used these to instruct AI tools to evaluate 73 studies published between 2011 and 2024. Our approach demonstrated the effectiveness of AI in extracting relevant information, interpreting the reliability of studies, and replicating human assessments. The findings indicate that AI-assisted assessments show promise in improving speed, consistency and applicability in QA/QC tasks, as well as in ranking studies or datasets based on their suitability for exposure and risk assessments. This groundbreaking application of LLMs in the environmental sciences suggests that AI can play a vital role in harmonizing microplastics risk assessments within regulatory frameworks and demonstrates how to meet the demands of an increasingly data-intensive application domain.

The widespread environmental presence of nanoplastics (NPs) raises significant concerns about their health impacts, particularly on the gastrointestinal system, as NPs are primarily ingested. While previous studies have linked NP-induced intestinal toxicity to oxidative stress and reactive oxygen species (ROS) accumulation, the specific mechanisms of cell death remain unclear. Here, we showed that environmentally relevant concentrations of polystyrene nanoplastics (PS-NPs) induced ferroptosis, a form of lipid peroxidation-driven cell death, in intestinal epithelial cells. Using intestinal epithelial-specific Nrf2-deficient mice (Nrf2fl/fl-VilCre+) and human intestinal epithelial Caco-2 cells, we demonstrated that Nrf2, a key oxidative stress regulator, play a protective role against PS-NP-induced ferroptosis. PS-NP exposure disrupted ether phospholipid metabolism, leading to the accumulation of polyunsaturated fatty acid-ether phospholipids and heightened lipid peroxidation in the intestines of Nrf2fl/fl-VilCre+ mice. This accumulation increased the susceptibility of intestinal epithelial cells to ferroptosis. Additionally, a high-fat diet further exacerbated this effect, suggesting that individuals with reduced NRF2 activity and poor dietary habits may be especially vulnerable to PS-NP-induced intestinal damage. Our findings offered new insights into the molecular mechanisms of NP-induced intestinal toxicity and underscored the health risks posed by environmental PS-NP exposure, particularly in populations with compromised antioxidant defenses.

Microplastics exposure can induce brain dysfunction like cognitive impairment, Parkinson's disease, and autism spectrum disorders. In this study, we aimed to investigate the effects of Polystyrene nanoplastics (NPS) on anxiety and depression in mice. First, Polystyrene nanoplastics (NPS) (10 mg/kg) were administered orally daily for two months starting at PND 21. Subsequently, behavioral tests about anxiety and depression were conducted, including the open field test, the elevated plus maze, the forced swimming test, and the tail suspension test. The results showed that NPS induced anxiety and depression-like behaviors in mice. The mPFC played a pivotal role in the etiology of anxiety and depression, in which nanoplastics led to impaired synaptic transmission and reduced neuronal activity in vivo in mPFC. Furthermore, the astrocyte marker GFAP was abnormally increased as observed in mPFC. The abnormal activation of astrocytes results in impaired glutamate recycling through decreasing the expression of the glutamate transporter protein EAAT2 after NPS exposure. In order to ascertain the function of EAAT2, the EAAT2 activator (LDN-212320) was employed to stimulate the expression of EAAT2. Following the activation of EAAT2, synaptic transmission, and anxiety and depressive behavior were rescued in the mice. Polystyrene nanoplastics induce anxiety and depressive-like behavior in mice possibly inhibiting astrocyte EAAT2 expression. Specific activation EAAT2 of astrocytes rescue anxiety and depressive behavior in nanoplastics exposed mice.