|
1
|
Okwumabua O, Bradley-Siemens N, Cruz C, Chittick L, Thompson M. Detection of SARS-CoV-2 and a possible variant in shelter cats. PLoS One 2025; 20:e0317104. [PMID: 39804893 PMCID: PMC11730420 DOI: 10.1371/journal.pone.0317104] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/22/2024] [Accepted: 12/20/2024] [Indexed: 01/16/2025] Open
Abstract
SARS-CoV-2 is the cause of mild to severe acute respiratory disease that led to significant loss of human lives worldwide between 2019 and 2022. The virus has been detected in various animals including cats and dogs making it a major public health concern and a One Health issue. In this study, conjunctival and pharyngeal swabs (n = 350) and serum samples (n = 350) were collected between July and December 2020 from cats that were housed in an animal shelter and tested for the infection of SARS-CoV-2 using real time reverse-transcription polymerase chain reaction (rRT-PCR) that targeted the N1 and N2 genes, and a SARS-CoV-2 surrogate virus neutralization Test (sVNT), respectively. 203 (58%) swab samples were negative (N1 and N2 not detected), 2 (0.6%) were positive (N1 and N2 detected) and 145 (41%) were inconclusive (only N1 detected). Analysis of the N2 region and multiple sequence alignment revealed base-pair deletions and substitutions in the N2 probe binding region of the feline samples RNA extracts in comparison with the positive control and human SARS-CoV-2 sequences in the GenBank database. Substituting the N2 probe with a probe derived from the cat sample amplicon sequences, 123 of 127 (96.9%) of the N2 negative samples returned positive. All but one of the 350 serum samples were negative for SARS-CoV-2 antibody. These observations indicated that although detection of SARS-CoV-2 infection was low in the samples tested, pet cats can harbor the virus and serve as potential source for virus spread that may lead to human infections. Additionally, cats may harbor a yet-to-be described virus that is somewhat related to SARS-CoV-2.
Collapse
Affiliation(s)
- Ogi Okwumabua
- Department of Pathology and Population Medicine, College of Veterinary Medicine, Midwestern University, Glendale, Arizona, United States of America
| | - Nancy Bradley-Siemens
- Department of Small Animal Shelter and Community Medicine, College of Veterinary Medicine, Midwestern University, Glendale, Arizona, United States of America
| | - Catherine Cruz
- Department of Pathology and Population Medicine, College of Veterinary Medicine, Midwestern University, Glendale, Arizona, United States of America
| | - Lauren Chittick
- Department of Pathology and Population Medicine, College of Veterinary Medicine, Midwestern University, Glendale, Arizona, United States of America
| | - Melissa Thompson
- Arizona Humane Society, Phoenix, Arizona, United States of America
| |
Collapse
|
|
2
|
Joseph OO, Dahunsi SO, Okoh A. SARS-CoV-2 infection of domestic animals and their role in evolution and emergence of variants of concern. New Microbes New Infect 2024; 62:101468. [PMID: 39268173 PMCID: PMC11391865 DOI: 10.1016/j.nmni.2024.101468] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/05/2024] [Revised: 05/31/2024] [Accepted: 08/22/2024] [Indexed: 09/15/2024] Open
Abstract
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that is responsible for COVID-19 pandemic, is a zoonotic RNA virus that has been reported in animals, including domestic animals. Due to the growing concern of health threat that could arise from active transmission of SARS-CoV-2 between pet owners and their pets, there is need to monitoring the emergence of a highly pathogenic strain of SARS-CoV-2 that is capable of transboundary infection, or a serious outbreak among human populations. Methods We carried out a search in English, on PubMed and NCBI (National Center for Biotechnology Information) SARS-CoV-2 resources for relevant journals and nucleotide sequence data, that were published between 2019 and 2023. The CoVsurver mutations application on GISAID webpage was used to analyse mutation, nucleotide sequence alignment was carried out using MAFFT (Multiple Alignment using Fast Fourier Transform) version 7 and maximum likelihood tree was constructed by bootstrapping with 1000 replicates on MEGA 11 software. Results A total of 47 mutations at the Spike gene region were identified, and mutation D614 was the most observed mutation. Nucleotide sequences of isolates from domestic animals had high sequence identity with Wuhan-Hu-1 reference sequence and the representative sequences of previously circulating VOCs from humans. Conclusion This reveals that there is spill over of previously circulating variants of concern (VOC) to household pets from their infected owners. Hence, there is an urgent need for more intense surveillance to be carried out globally to monitor evolution of SARS-CoV-2 coronaviruses as a result of human - pet association.
Collapse
Affiliation(s)
| | - Samuel Olatunde Dahunsi
- Microbiology Programme, College of Agriculture, Engineering, and Sciences, Bowen University Iwo, Osun State Nigeria
- The Radcliffe Institute for Advanced Study, Harvard University, Cambridge, MA, USA
| | - Anthony Okoh
- SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, South Africa
| |
Collapse
|
|
3
|
Silva PV, Nobre CN. Computational methods in the analysis of SARS-CoV-2 in mammals: A systematic review of the literature. Comput Biol Med 2024; 173:108264. [PMID: 38564853 DOI: 10.1016/j.compbiomed.2024.108264] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/28/2023] [Revised: 02/15/2024] [Accepted: 03/06/2024] [Indexed: 04/04/2024]
Abstract
SARS-CoV-2 is an enveloped RNA virus that causes severe respiratory illness in humans and animals. It infects cells by binding the Spike protein to the host's angiotensin-converting enzyme 2 (ACE2). The bat is considered the natural host of the virus, and zoonotic transmission is a significant risk and can happen when humans come into close contact with infected animals. Therefore, understanding the interconnection between human, animal, and environmental health is important to prevent and control future coronavirus outbreaks. This work aimed to systematically review the literature to identify characteristics that make mammals suitable virus transmitters and raise the main computational methods used to evaluate SARS-CoV-2 in mammals. Based on this review, it was possible to identify the main factors related to transmissions mentioned in the literature, such as the expression of ACE2 and proximity to humans, in addition to identifying the computational methods used for its study, such as Machine Learning, Molecular Modeling, Computational Simulation, between others. The findings of the work contribute to the prevention and control of future outbreaks, provide information on transmission factors, and highlight the importance of advanced computational methods in the study of infectious diseases that allow a deeper understanding of transmission patterns and can help in the development of more effective control and intervention strategies.
Collapse
Affiliation(s)
- Paula Vitória Silva
- Pontifical Catholic University of Minas Gerais - PUC Minas, 500 Dom José Gaspar Street, Building 41, Coração Eucarístico, Belo Horizonte, MG 30535-901, Brazil.
| | - Cristiane N Nobre
- Pontifical Catholic University of Minas Gerais - PUC Minas, 500 Dom José Gaspar Street, Building 41, Coração Eucarístico, Belo Horizonte, MG 30535-901, Brazil.
| |
Collapse
|
|
4
|
Silva MJA, Santana DS, Lima MBM, Silva CS, de Oliveira LG, Monteiro EOL, Dias RDS, Pereira BDKB, Nery PADS, Ferreira MAS, Sarmento MADS, Ayin AAN, Mendes de Oliveira AC, Lima KVB, Lima LNGC. Assessment of the Risk Impact of SARS-CoV-2 Infection Prevalence between Cats and Dogs in America and Europe: A Systematic Review and Meta-Analysis. Pathogens 2024; 13:314. [PMID: 38668269 PMCID: PMC11053406 DOI: 10.3390/pathogens13040314] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/01/2024] [Revised: 03/21/2024] [Accepted: 03/22/2024] [Indexed: 04/29/2024] Open
Abstract
The COVID-19 pandemic represented a huge obstacle for public health and demonstrated weaknesses in surveillance and health promotion systems around the world. Its etiological agent, SARS-CoV-2, of zoonotic origin, has been the target of several studies related to the control and prevention of outbreaks and epidemics of COVID-19 not only for humans but also for animals. Domestic animals, such as dogs and cats, have extensive contact with humans and can acquire the infection both naturally and directly from humans. The objective of this article was to summarize the seroprevalence findings of SARS-CoV-2 in dogs and cats and correlate them with the strength of infection risk between each of them. This is a systematic review and meta-analysis following the recommendations of PRISMA 2020. The search and selection of papers was carried out using in vivo experimental works with animals using the descriptors (MeSH/DeCS) "Animal", "Public Health", "SARS-CoV-2" and "Pandemic" (together with AND) in English, Portuguese or Spanish for Science Direct, PUBMED, LILACS and SciELO databases. The ARRIVE checklist was used for methodological evaluation and the Comprehensive Meta-Analysis v2.2 software with the Difference Risk (RD) test to evaluate statistical inferences (with subgroups by continent). Cats showed greater susceptibility to SARS-CoV-2 compared to dogs both in a joint analysis of studies (RD = 0.017; 95% CI = 0.008-0.025; p < 0.0001) and in the American subgroup (RD = 0.053; 95% CI = 0.032-0.073; p < 0.0001), unlike the lack of significant difference on the European continent (RD = 0.009; 95% CI = -0.001-0.018; p = 0.066). Therefore, it was observed that cats have a greater interest in health surveillance due to the set of biological and ecological aspects of these animals, but also that there are a set of factors that can influence the spread and possible spillover events of the virus thanks to the anthropozoonotic context.
Collapse
Affiliation(s)
- Marcos Jessé Abrahão Silva
- Center for Biological and Health Sciences (CCBS), University of the State of Pará (UEPA), Belém 66087-670, PA, Brazil;
| | - Davi Silva Santana
- Institute of Health Sciences (ICS), Institute of Biological Sciences (ICB), Federal University of Pará (UFPA), Belém 66077-830, PA, Brazil; (D.S.S.); (M.B.M.L.); (R.d.S.D.); (M.A.S.F.); (A.C.M.d.O.)
| | - Marceli Batista Martins Lima
- Institute of Health Sciences (ICS), Institute of Biological Sciences (ICB), Federal University of Pará (UFPA), Belém 66077-830, PA, Brazil; (D.S.S.); (M.B.M.L.); (R.d.S.D.); (M.A.S.F.); (A.C.M.d.O.)
| | - Caroliny Soares Silva
- Center for Biological and Health Sciences (CCBS), University of the State of Pará (UEPA), Belém 66087-670, PA, Brazil;
| | - Letícia Gomes de Oliveira
- Evandro Chagas Institute (IEC), Ananindeua 67030-000, PA, Brazil; (L.G.d.O.); (K.V.B.L.); (L.N.G.C.L.)
| | | | - Rafael dos Santos Dias
- Institute of Health Sciences (ICS), Institute of Biological Sciences (ICB), Federal University of Pará (UFPA), Belém 66077-830, PA, Brazil; (D.S.S.); (M.B.M.L.); (R.d.S.D.); (M.A.S.F.); (A.C.M.d.O.)
| | - Bruna de Kássia Barbosa Pereira
- Department of Veterinary Medicine, University of the Amazon (UNAMA), Belém 66120-901, PA, Brazil; (B.d.K.B.P.); (P.A.d.S.N.)
| | - Paula Andresa da Silva Nery
- Department of Veterinary Medicine, University of the Amazon (UNAMA), Belém 66120-901, PA, Brazil; (B.d.K.B.P.); (P.A.d.S.N.)
| | - Márcio André Silva Ferreira
- Institute of Health Sciences (ICS), Institute of Biological Sciences (ICB), Federal University of Pará (UFPA), Belém 66077-830, PA, Brazil; (D.S.S.); (M.B.M.L.); (R.d.S.D.); (M.A.S.F.); (A.C.M.d.O.)
| | | | - Andrea Alexandra Narro Ayin
- Faculty of Medicine, Centro Universitário do Estado do Pará (CESUPA), Belém 66613-903, PA, Brazil; (M.A.d.S.S.); (A.A.N.A.)
| | - Ana Cristina Mendes de Oliveira
- Institute of Health Sciences (ICS), Institute of Biological Sciences (ICB), Federal University of Pará (UFPA), Belém 66077-830, PA, Brazil; (D.S.S.); (M.B.M.L.); (R.d.S.D.); (M.A.S.F.); (A.C.M.d.O.)
| | | | | |
Collapse
|
|
5
|
Nilsson MG, Santana Cordeiro MDC, Gonçalves ACA, Dos Santos Conzentino M, Huergo LF, Vicentini F, Reis JBL, Biondo AW, Kmetiuk LB, da Silva AV. High seroprevalence for SARS-CoV-2 infection in dogs: Age as risk factor for infection in shelter and foster home animals. Prev Vet Med 2024; 222:106094. [PMID: 38103433 DOI: 10.1016/j.prevetmed.2023.106094] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/02/2023] [Revised: 11/30/2023] [Accepted: 12/02/2023] [Indexed: 12/19/2023]
Abstract
SARS-CoV-2 has caused 775 outbreaks in 29 animal species across 36 countries, including dogs, cats, ferrets, minks, non-human primates, white-tailed deer, and lions. Although transmission from owners to dogs has been extensively described, no study to date has also compared sheltered, foster home and owner dogs and associated risk factors. This study aimed to identify SARS-CoV-2 infection and anti-SARS-CoV-2 antibodies from sheltered, fostered, and owned dogs, associated with environmental and management risk factors. Serum samples and swabs were collected from each dog, and an epidemiological questionnaire was completed by the shelter manager, foster care, and owner. A total of 111 dogs, including 222 oropharyngeal and rectal swabs, tested negative by RT-qPCR. Overall, 18/89 (20.22%) dogs presented IgG antibodies against the N protein of SARS-CoV-2 by magnetic ELISA, while none showed a reaction to the Spike protein. SARS-CoV-2 antibodies showed an age-related association, with 4.16 chance of positivity in adult dogs when compared with young ones. High population density among dogs and humans, coupled with repeated COVID-19 exposure, emerged as potential risk factors in canine virus epidemiology. Dogs exhibited higher seropositivity rates in these contexts. Thus, we propose expanded seroepidemiological and molecular studies across species and scenarios, including shelter dogs.
Collapse
Affiliation(s)
- Mariana Guimarães Nilsson
- Graduate College of Animal Science in the Tropics, Federal University of Bahia (UFBA), 40170-110 Salvador, Bahia, Brazil.
| | | | | | | | | | - Fernando Vicentini
- Health Sciences Center, Federal University of the Recôncavo of Bahia (UFRB), 44430-622 Santo Antônio de Jesus, Bahia, Brazil
| | - Jeiza Botelho Leal Reis
- Health Sciences Center, Federal University of the Recôncavo of Bahia (UFRB), 44430-622 Santo Antônio de Jesus, Bahia, Brazil
| | - Alexander Welker Biondo
- Graduate College of Cellular and Molecular Biology, Federal University of Paraná (UFPR), 81531-970 Curitiba, Paraná, Brazil
| | - Louise Bach Kmetiuk
- Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Paraná 81310-020, Brazil
| | - Aristeu Vieira da Silva
- Zoonosis and Public Health Research Group, Earth and Environmental Science Modelling Graduate, State University of Feira de Santana (UEFS), 44036-900 Feira de Santana, Bahia, Brazil.
| |
Collapse
|
|
6
|
Chen D, López‐Pérez AM, Vernau KM, Maggs DJ, Kim S, Foley J. Prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and feline enteric coronavirus (FECV) in shelter-housed cats in the Central Valley of California, USA. Vet Rec Open 2023; 10:e73. [PMID: 37868705 PMCID: PMC10589393 DOI: 10.1002/vro2.73] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/08/2023] [Revised: 08/24/2023] [Accepted: 09/19/2023] [Indexed: 10/24/2023] Open
Abstract
Background Non-human animals are natural hosts for the virus causing COVID-19 (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) and a diversity of species appear susceptible to infection. Cats are of particular concern because of their close affiliation with humans and susceptibility to infection. Cats also harbour feline enteric coronavirus (FECV). Our objectives were to document the prevalence of SARS-CoV-2 and FECV in feline populations with high turnover and movement among households in the Central Valley of California, USA. Methods A cross-sectional study of 128 shelter and foster cats and kittens in the Central Valley of California was performed from July to December 2020. PCR was performed on rectal and oropharyngeal samples to detect SARS-CoV-2 RNA and on rectal samples to detect FECV RNA. Results Among 163 rectal and oropharyngeal fluid samples gathered from sheltered and fostered cats and kittens in central California, SARS-CoV-2 nucleic acids were not detected from any cat or kitten. In contrast, FECV nucleic acids were detected in 18% of shelter-housed cats; 83% of these positive samples were collected from cats housed in adjacent cages. Conclusions These data may be helpful when considering the allocation of resources to minimise the harm of FECV and SARS-CoV-2 in household pets and shelter environments.
Collapse
Affiliation(s)
- Daniel Chen
- Department of Medicine and EpidemiologySchool of Veterinary MedicineUniversity of CaliforniaDavisCaliforniaUSA
| | - Andrés M. López‐Pérez
- Department of Medicine and EpidemiologySchool of Veterinary MedicineUniversity of CaliforniaDavisCaliforniaUSA
- Red de Biología y Conservación de VertebradosInstituto de EcologíaXalapaMéxico
| | - Karen M. Vernau
- Department of Surgical and Radiological SciencesSchool of Veterinary MedicineUniversity of CaliforniaDavisCaliforniaUSA
| | - David J. Maggs
- Department of Surgical and Radiological SciencesSchool of Veterinary MedicineUniversity of CaliforniaDavisCaliforniaUSA
| | - Soohyun Kim
- William R. Pritchard Veterinary Medical Teaching HospitalSchool of Veterinary MedicineUniversity of CaliforniaDavisCaliforniaUSA
| | - Janet Foley
- Department of Medicine and EpidemiologySchool of Veterinary MedicineUniversity of CaliforniaDavisCaliforniaUSA
| |
Collapse
|
|
7
|
Tyson GB, Jones S, Montreuil-Spencer C, Logan N, Scott S, Sasvari H, McDonald M, Marshall L, Murcia PR, Willett BJ, Weir W, Hosie MJ. Increase in SARS-CoV-2 Seroprevalence in UK Domestic Felids Despite Weak Immunogenicity of Post-Omicron Variants. Viruses 2023; 15:1661. [PMID: 37632004 PMCID: PMC10458763 DOI: 10.3390/v15081661] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/28/2023] [Revised: 07/25/2023] [Accepted: 07/28/2023] [Indexed: 08/27/2023] Open
Abstract
Throughout the COVID-19 pandemic, SARS-CoV-2 infections in domestic cats have caused concern for both animal health and the potential for inter-species transmission. Cats are known to be susceptible to the Omicron variant and its descendants, however, the feline immune response to these variants is not well defined. We aimed to estimate the current seroprevalence of SARS-CoV-2 in UK pet cats, as well as characterise the neutralising antibody response to the Omicron (BA.1) variant. A neutralising seroprevalence of 4.4% and an overall seroprevalence of 13.9% was observed. Both purebred and male cats were found to have the highest levels of seroprevalence, as well as cats aged between two and five years. The Omicron variant was found to have a lower immunogenicity in cats than the B.1, Alpha and Delta variants, which reflects previous reports of immune and vaccine evasion in humans. These results further underline the importance of surveillance of SARS-CoV-2 infections in UK cats as the virus continues to evolve.
Collapse
Affiliation(s)
- Grace B. Tyson
- MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
- School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, UK
| | - Sarah Jones
- MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
- School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, UK
| | - Chloe Montreuil-Spencer
- School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, UK
| | - Nicola Logan
- MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
| | - Sam Scott
- MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
| | - Hagar Sasvari
- MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
| | - Michael McDonald
- School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, UK
| | - Leigh Marshall
- School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, UK
| | - Pablo R. Murcia
- MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
| | - Brian J. Willett
- MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
| | - William Weir
- School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, UK
| | - Margaret J. Hosie
- MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
- School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, UK
| |
Collapse
|
|
8
|
High seroprevalence of SARS-CoV-2 antibodies in household cats and dogs of Lebanon. Res Vet Sci 2023; 157:13-16. [PMID: 36842247 PMCID: PMC9942449 DOI: 10.1016/j.rvsc.2023.02.005] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/30/2022] [Revised: 02/16/2023] [Accepted: 02/19/2023] [Indexed: 02/23/2023]
Abstract
The COVID-19 pandemic has been declared in late 2019. It is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Flu-like symptoms and acute respiratory illnesses are the main manifestations of the disease. Recent studies have confirmed the susceptibility of domestic animals to SARS-CoV-2 infection. However, the seroprevalence of SARS-CoV-2 in household pets and the importance of pets in the epidemiology of this infection remain unknown. In Lebanon, there is no epidemiological data regarding SARS-CoV-2 infection in companion animals. Thus, this investigation aimed to determine the seroprevalence of SARS-CoV-2 antibodies in household pets of Lebanon during the COVID-19 pandemic. A cross-sectional study was carried out between April 2020 and February 2021. Blood samples from 145 cats and 180 dogs were collected from 12 veterinary clinics located in the North, Mount, and Beirut governorates. A validated ELISA assay was used to detect the anti- SARS-CoV-2 in the sera of the tested animals. An overall seroprevalence of 16.92% (55/325) was reported; 13.79% seroprevalence was found in cats (20/145) and 19.44% (35/180) in dogs. The young age and the cold season were significantly associated with an increased seropositivity rate to SARS-CoV-2 infection (P < 0.01). These results confirm the circulation of SARS-CoV-2 in household pets, in various geographical regions in Lebanon. Although, there is a lack of evidence to suggest that naturally infected pets could transmit the SARS-CoV-2 infection. Yet, owners diagnosed with COVID-19 should limit their contact with their animals during the course of the disease to curb the risk of transmission.
Collapse
|
|
9
|
Dunowska M. Cross-species transmission of coronaviruses with a focus on severe acute respiratory syndrome coronavirus 2 infection in animals: a review for the veterinary practitioner. N Z Vet J 2023:1-13. [PMID: 36927253 DOI: 10.1080/00480169.2023.2191349] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/18/2023]
Abstract
AbstractIn 2019 a novel coronavirus termed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged from an unidentified source and spread rapidly among humans worldwide. While many human infections are mild, some result in severe clinical disease that in a small proportion of infected people is fatal. The pandemic spread of SARS-CoV-2 has been facilitated by efficient human-to-human transmission of the virus, with no data to indicate that animals contributed to this global health crisis. However, a range of domesticated and wild animals are also susceptible to SARS-CoV-2 infection under both experimental and natural conditions. Humans are presumed to be the source of most animal infections thus far, although natural transmission between mink and between free-ranging deer has occurred, and occasional natural transmission between cats cannot be fully excluded. Considering the ongoing circulation of the virus among people, together with its capacity to evolve through mutation and recombination, the risk of the emergence of animal-adapted variants is not negligible. If such variants remain infectious to humans, this could lead to the establishment of an animal reservoir for the virus, which would complicate control efforts. As such, minimising human-to-animal transmission of SARS-CoV-2 should be considered as part of infection control efforts. The aim of this review is to summarise what is currently known about the species specificity of animal coronaviruses, with an emphasis on SARS-CoV-2, in the broader context of factors that facilitate cross-species transmission of viruses.
Collapse
Affiliation(s)
- M Dunowska
- Tāwharau Ora - School of Veterinary Science, Massey University, Palmerston North, New Zealand
| |
Collapse
|
|
10
|
Zehr JD, Kosakovsky Pond SL, Millet JK, Olarte-Castillo XA, Lucaci AG, Shank SD, Ceres KM, Choi A, Whittaker GR, Goodman LB, Stanhope MJ. Natural selection differences detected in key protein domains between non-pathogenic and pathogenic feline coronavirus phenotypes. Virus Evol 2023; 9:vead019. [PMID: 37038392 PMCID: PMC10082545 DOI: 10.1093/ve/vead019] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/09/2023] [Revised: 02/14/2023] [Accepted: 03/13/2023] [Indexed: 03/17/2023] Open
Abstract
Feline coronaviruses (FCoVs) commonly cause mild enteric infections in felines worldwide (termed feline enteric coronavirus [FECV]), with around 12 per cent developing into deadly feline infectious peritonitis (FIP; feline infectious peritonitis virus [FIPV]). Genomic differences between FECV and FIPV have been reported, yet the putative genotypic basis of the highly pathogenic phenotype remains unclear. Here, we used state-of-the-art molecular evolutionary genetic statistical techniques to identify and compare differences in natural selection pressure between FECV and FIPV sequences, as well as to identify FIPV- and FECV-specific signals of positive selection. We analyzed full-length FCoV protein coding genes thought to contain mutations associated with FIPV (Spike, ORF3abc, and ORF7ab). We identified two sites exhibiting differences in natural selection pressure between FECV and FIPV: one within the S1/S2 furin cleavage site (FCS) and the other within the fusion domain of Spike. We also found fifteen sites subject to positive selection associated with FIPV within Spike, eleven of which have not previously been suggested as possibly relevant to FIP development. These sites fall within Spike protein subdomains that participate in host cell receptor interaction, immune evasion, tropism shifts, host cellular entry, and viral escape. There were fourteen sites (twelve novel sites) within Spike under positive selection associated with the FECV phenotype, almost exclusively within the S1/S2 FCS and adjacent to C domain, along with a signal of relaxed selection in FIPV relative to FECV, suggesting that furin cleavage functionality may not be needed for FIPV. Positive selection inferred in ORF7b was associated with the FECV phenotype and included twenty-four positively selected sites, while ORF7b had signals of relaxed selection in FIPV. We found evidence of positive selection in ORF3c in FCoV-wide analyses, but no specific association with the FIPV or FECV phenotype. We hypothesize that some combination of mutations in FECV may contribute to FIP development, and that it is unlikely to be one singular 'switch' mutational event. This work expands our understanding of the complexities of FIP development and provides insights into how evolutionary forces may alter pathogenesis in coronavirus genomes.
Collapse
Affiliation(s)
- Jordan D Zehr
- Department of Biology, Temple University, Institute for Genomics and Evolutionary Medicine, Philadelphia, PA 19122, USA
| | - Sergei L Kosakovsky Pond
- Department of Biology, Temple University, Institute for Genomics and Evolutionary Medicine, Philadelphia, PA 19122, USA
| | - Jean K Millet
- Université Paris-Saclay, INRAE, UVSQ, Virologie et Immunologie Moléculaires, Jouy-en-Josas 78352, France
| | - Ximena A Olarte-Castillo
- Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
- James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
| | - Alexander G Lucaci
- Department of Biology, Temple University, Institute for Genomics and Evolutionary Medicine, Philadelphia, PA 19122, USA
| | - Stephen D Shank
- Department of Biology, Temple University, Institute for Genomics and Evolutionary Medicine, Philadelphia, PA 19122, USA
| | - Kristina M Ceres
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
| | - Annette Choi
- Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
| | - Gary R Whittaker
- Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
| | - Laura B Goodman
- James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
| | - Michael J Stanhope
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
| |
Collapse
|
|
11
|
Conforti A, Sanchez E, Salvatori E, Lione L, Compagnone M, Pinto E, Palombo F, D’Acunto E, Muzi A, Roscilli G, Sun Y, Viscount B, Hayward J, Shorrock C, Diel DG, Impellizeri JA, Aurisicchio L. A linear DNA encoding the SARS-CoV-2 receptor binding domain elicits potent immune response and neutralizing antibodies in domestic cats. Mol Ther Methods Clin Dev 2023; 28:238-248. [PMID: 36618106 PMCID: PMC9806924 DOI: 10.1016/j.omtm.2022.12.015] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/03/2022] [Accepted: 12/31/2022] [Indexed: 01/03/2023]
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiologic agent of the COVID-19 pandemic, has been shown to infect a wide range of animal species, especially mammals, and besides human-to-human transmission, human-to-animal transmission has also been observed in some wild animals and pets, especially in cats. It has been demonstrated that cats are permissive to COVID-19 and are susceptible to airborne infections. Given the high transmissibility potential of SARS-CoV-2 to different host species and the close contact between humans and animals, it is crucial to find mechanisms to prevent the transmission chain and reduce the risk of spillover to susceptible species. Here, we show results from a clinical trial conducted in domestic cats to assess safety and immunogenicity of a linear DNA (linDNA) vaccine encoding the receptor-binding domain (RBD) from SARS-CoV-2 (Lin-COVID-eVax). Lin-COVID-eVax proved to be safe, with no significant adverse events, and was able to elicit both RBD-specific antibodies and T cells. Also, the linDNA vaccine induced neutralizing antibody titers against ancestral SARS-CoV-2 virus and its variants. These findings demonstrate the safety and immunogenicity of a genetic vaccine against COVID-19 administered to cats and strongly support the development of vaccines for preventing viral spread in susceptible species, especially those in close contact with humans.
Collapse
Affiliation(s)
- Antonella Conforti
- Takis Biotech, Via Castel Romano 100, 00128 Rome, Italy
- Evvivax Biotech, Via Castel Romano 100, 00128 Rome, Italy
| | - Elisa Sanchez
- Veterinary Oncology Services (VOS), PLLC, New York, NY, USA
| | | | - Lucia Lione
- Takis Biotech, Via Castel Romano 100, 00128 Rome, Italy
| | | | | | - Fabio Palombo
- Takis Biotech, Via Castel Romano 100, 00128 Rome, Italy
- Neomatrix Biotech, Via Castel Romano 100, 00128 Rome, Italy
| | | | - Alessia Muzi
- Takis Biotech, Via Castel Romano 100, 00128 Rome, Italy
| | | | - Yuhua Sun
- Applied DNA Sciences, Inc., 50 Health Sciences Drive, Stony Brook, NY 11790, USA
- LineaRx, Inc., 50 Health Sciences Drive, Stony Brook, NY 11790, USA
| | - Brian Viscount
- Applied DNA Sciences, Inc., 50 Health Sciences Drive, Stony Brook, NY 11790, USA
- LineaRx, Inc., 50 Health Sciences Drive, Stony Brook, NY 11790, USA
| | - James Hayward
- Applied DNA Sciences, Inc., 50 Health Sciences Drive, Stony Brook, NY 11790, USA
- LineaRx, Inc., 50 Health Sciences Drive, Stony Brook, NY 11790, USA
| | - Clay Shorrock
- Applied DNA Sciences, Inc., 50 Health Sciences Drive, Stony Brook, NY 11790, USA
- LineaRx, Inc., 50 Health Sciences Drive, Stony Brook, NY 11790, USA
| | - Diego G. Diel
- Department of Population Medicine and Diagnostic Sciences, Animal Health Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA
| | | | - Luigi Aurisicchio
- Takis Biotech, Via Castel Romano 100, 00128 Rome, Italy
- Evvivax Biotech, Via Castel Romano 100, 00128 Rome, Italy
- Neomatrix Biotech, Via Castel Romano 100, 00128 Rome, Italy
| |
Collapse
|
|
12
|
Zhou Q, Luo Y, Zhu Y, Chen Q, Qiu J, Cong F, Li Y, Zhang X. Nonsteroidal anti-inflammatory drugs (NSAIDs) and nucleotide analog GS-441524 conjugates with potent in vivo efficacy against coronaviruses. Eur J Med Chem 2023; 249:115113. [PMID: 36706621 PMCID: PMC9830933 DOI: 10.1016/j.ejmech.2023.115113] [Citation(s) in RCA: 5] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/17/2022] [Revised: 12/25/2022] [Accepted: 01/09/2023] [Indexed: 01/11/2023]
Abstract
Coronaviruses (CoVs) infect a broad range of hosts, including humans and various animals, with a tendency to cross the species barrier, causing severe harm to human society and fostering the need for effective anti-coronaviral drugs. GS-441524 is a broad-spectrum antiviral nucleoside with potent anti-CoVs activities. However, its application is limited by poor oral bioavailability. Herein, we designed and synthesized several conjugates via covalently binding NSAIDs to 5'-OH of GS-441524 through ester bonds. The ibuprofen conjugate, ATV041, exhibited potent in vitro anti-coronaviral efficacy against four zoonotic coronaviruses in the alpha- and beta-genera. Oral-dosed ATV041 resulted in favorable bioavailability and rapid tissue distribution of GS-441524 and ibuprofen. In MHV-A59 infected mice, ATV041 dose-dependently decreased viral RNA replication and significantly reduced the proinflammatory cytokines in the liver and the lung at 3 dpi. As a result, the MHV-A59-induced lung and liver inflammatory injury was significantly alleviated. Taken together, this work provides a novel drug conjugate strategy to improve oral PK and offers a potent anti-coronaviral lead compound for further studies.
Collapse
Affiliation(s)
- Qifan Zhou
- Department of Chemistry, College of Science, Academy for Advanced Interdisciplinary Studies and Medi-X Pingshan, Southern University of Science and Technology, Shenzhen, Guangdong, 518000, China
| | - Yinzhu Luo
- Guangdong Province Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, Guangdong, 510663, China
| | - Yujun Zhu
- Guangdong Province Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, Guangdong, 510663, China
| | - Qishu Chen
- Department of Chemistry, College of Science, Academy for Advanced Interdisciplinary Studies and Medi-X Pingshan, Southern University of Science and Technology, Shenzhen, Guangdong, 518000, China
| | - Jingfei Qiu
- Department of Chemistry, College of Science, Academy for Advanced Interdisciplinary Studies and Medi-X Pingshan, Southern University of Science and Technology, Shenzhen, Guangdong, 518000, China
| | - Feng Cong
- Guangdong Province Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, Guangdong, 510663, China.
| | - Yingjun Li
- Department of Chemistry, College of Science, Academy for Advanced Interdisciplinary Studies and Medi-X Pingshan, Southern University of Science and Technology, Shenzhen, Guangdong, 518000, China; State Key Laboratory of Chemical Oncogenomics, Tsinghua Shenzhen International Graduate School, Shenzhen, 518055, China.
| | - Xumu Zhang
- Department of Chemistry, College of Science, Academy for Advanced Interdisciplinary Studies and Medi-X Pingshan, Southern University of Science and Technology, Shenzhen, Guangdong, 518000, China.
| |
Collapse
|
|
13
|
Mironov AA, Savin MA, Beznoussenko GV. COVID-19 Biogenesis and Intracellular Transport. Int J Mol Sci 2023; 24:ijms24054523. [PMID: 36901955 PMCID: PMC10002980 DOI: 10.3390/ijms24054523] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/04/2023] [Revised: 02/13/2023] [Accepted: 02/20/2023] [Indexed: 03/02/2023] Open
Abstract
SARS-CoV-2 is responsible for the COVID-19 pandemic. The structure of SARS-CoV-2 and most of its proteins of have been deciphered. SARS-CoV-2 enters cells through the endocytic pathway and perforates the endosomes' membranes, and its (+) RNA appears in the cytosol. Then, SARS-CoV-2 starts to use the protein machines of host cells and their membranes for its biogenesis. SARS-CoV-2 generates a replication organelle in the reticulo-vesicular network of the zippered endoplasmic reticulum and double membrane vesicles. Then, viral proteins start to oligomerize and are subjected to budding within the ER exit sites, and its virions are passed through the Golgi complex, where the proteins are subjected to glycosylation and appear in post-Golgi carriers. After their fusion with the plasma membrane, glycosylated virions are secreted into the lumen of airways or (seemingly rarely) into the space between epithelial cells. This review focuses on the biology of SARS-CoV-2's interactions with cells and its transport within cells. Our analysis revealed a significant number of unclear points related to intracellular transport in SARS-CoV-2-infected cells.
Collapse
Affiliation(s)
- Alexander A. Mironov
- Department of Cell Biology, IFOM ETS—The AIRC Institute of Molecular Oncology, Via Adamello, 16, 20139 Milan, Italy
- Correspondence:
| | - Maksim A. Savin
- The Department for Welding Production and Technology of Constructional Materials, Perm National Research Polytechnic University, Komsomolsky Prospekt, 29, 614990 Perm, Russia
| | - Galina V. Beznoussenko
- Department of Cell Biology, IFOM ETS—The AIRC Institute of Molecular Oncology, Via Adamello, 16, 20139 Milan, Italy
| |
Collapse
|
|
14
|
Santaniello A, Perruolo G, Cristiano S, Agognon AL, Cabaro S, Amato A, Dipineto L, Borrelli L, Formisano P, Fioretti A, Oriente F. SARS-CoV-2 Affects Both Humans and Animals: What Is the Potential Transmission Risk? A Literature Review. Microorganisms 2023; 11:microorganisms11020514. [PMID: 36838479 PMCID: PMC9959838 DOI: 10.3390/microorganisms11020514] [Citation(s) in RCA: 6] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/30/2022] [Revised: 02/14/2023] [Accepted: 02/16/2023] [Indexed: 02/22/2023] Open
Abstract
In March 2020, the World Health Organization Department declared the coronavirus (COVID-19) outbreak a global pandemic, as a consequence of its rapid spread on all continents. The COVID-19 pandemic has been not only a health emergency but also a serious general problem as fear of contagion and severe restrictions put economic and social activity on hold in many countries. Considering the close link between human and animal health, COVID-19 might infect wild and companion animals, and spawn dangerous viral mutants that could jump back and pose an ulterior threat to us. The purpose of this review is to provide an overview of the pandemic, with a particular focus on the clinical manifestations in humans and animals, the different diagnosis methods, the potential transmission risks, and their potential direct impact on the human-animal relationship.
Collapse
Affiliation(s)
- Antonio Santaniello
- Department of Veterinary Medicine and Animal Production, Federico II University of Naples, 80134 Naples, Italy
- Correspondence: (A.S.); (S.C.); Tel.: +39-081-253-6134 (A.S.)
| | - Giuseppe Perruolo
- Department of Translational Medical Sciences, Federico II University of Naples, 80131 Naples, Italy
| | - Serena Cristiano
- Department of Veterinary Medicine and Animal Production, Federico II University of Naples, 80134 Naples, Italy
- Correspondence: (A.S.); (S.C.); Tel.: +39-081-253-6134 (A.S.)
| | - Ayewa Lawoe Agognon
- Department of Translational Medical Sciences, Federico II University of Naples, 80131 Naples, Italy
| | - Serena Cabaro
- Department of Translational Medical Sciences, Federico II University of Naples, 80131 Naples, Italy
| | - Alessia Amato
- Department of Veterinary Medicine and Animal Production, Federico II University of Naples, 80134 Naples, Italy
| | - Ludovico Dipineto
- Department of Veterinary Medicine and Animal Production, Federico II University of Naples, 80134 Naples, Italy
| | - Luca Borrelli
- Department of Veterinary Medicine and Animal Production, Federico II University of Naples, 80134 Naples, Italy
| | - Pietro Formisano
- Department of Translational Medical Sciences, Federico II University of Naples, 80131 Naples, Italy
| | - Alessandro Fioretti
- Department of Veterinary Medicine and Animal Production, Federico II University of Naples, 80134 Naples, Italy
| | - Francesco Oriente
- Department of Translational Medical Sciences, Federico II University of Naples, 80131 Naples, Italy
| |
Collapse
|
|
15
|
Interactions between Humans and Dogs during the COVID-19 Pandemic: Recent Updates and Future Perspectives. Animals (Basel) 2023; 13:ani13030524. [PMID: 36766413 PMCID: PMC9913536 DOI: 10.3390/ani13030524] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/13/2022] [Revised: 01/27/2023] [Accepted: 01/30/2023] [Indexed: 02/05/2023] Open
Abstract
COVID-19 is one of the deadliest epidemics. This pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but the role of dogs in spreading the disease in human society is poorly understood. This review sheds light on the limited susceptibility of dogs to COVID-19 infections which is likely attributed to the relatively low levels of angiotensin-converting enzyme 2 (ACE2) in the respiratory tract and the phylogenetic distance of ACE2 in dogs from the human ACE2 receptor. The low levels of ACE2 affect the binding affinity between spike and ACE2 proteins resulting in it being uncommon for dogs to spread the disease. To demonstrate the role of dogs in spreading COVID-19, we reviewed the epidemiological studies and prevalence of SARS-CoV-2 in dogs. Additionally, we discussed the use of detection dogs as a rapid and reliable method for effectively discriminating between SARS-CoV-2 infected and non-infected individuals using different types of samples (secretions, saliva, and sweat). We considered the available information on COVID-19 in the human-dog interfaces involving the possibility of transmission of COVID-19 to dogs by infected individuals and vice versa, the human-dog behavior changes, and the importance of preventive measures because the risk of transmission by domestic dogs remains a concern.
Collapse
|
|
16
|
Abstract
The existence of coronaviruses has been known for many years. These viruses cause significant disease that primarily seems to affect agricultural species. Human coronavirus disease due to the 2002 outbreak of Severe Acute Respiratory Syndrome and the 2012 outbreak of Middle East Respiratory Syndrome made headlines; however, these outbreaks were controlled, and public concern quickly faded. This complacency ended in late 2019 when alarms were raised about a mysterious virus responsible for numerous illnesses and deaths in China. As we now know, this novel disease called Coronavirus Disease 2019 (COVID-19) was caused by Severe acute respiratory syndrome-related-coronavirus-2 (SARS-CoV-2) and rapidly became a worldwide pandemic. Luckily, decades of research into animal coronaviruses hastened our understanding of the genetics, structure, transmission, and pathogenesis of these viruses. Coronaviruses infect a wide range of wild and domestic animals, with significant economic impact in several agricultural species. Their large genome, low dependency on host cellular proteins, and frequent recombination allow coronaviruses to successfully cross species barriers and adapt to different hosts including humans. The study of the animal diseases provides an understanding of the virus biology and pathogenesis and has assisted in the rapid development of the SARS-CoV-2 vaccines. Here, we briefly review the classification, origin, etiology, transmission mechanisms, pathogenesis, clinical signs, diagnosis, treatment, and prevention strategies, including available vaccines, for coronaviruses that affect domestic, farm, laboratory, and wild animal species. We also briefly describe the coronaviruses that affect humans. Expanding our knowledge of this complex group of viruses will better prepare us to design strategies to prevent and/or minimize the impact of future coronavirus outbreaks.
Collapse
Key Words
- bcov, bovine coronavirus
- ccov, canine coronavirus
- cov(s), coronavirus(es)
- covid-19, coronavirus disease 2019
- crcov, canine respiratory coronavirus
- e, coronaviral envelope protein
- ecov, equine coronavirus
- fcov, feline coronavirus
- fipv, feline infectious peritonitis virus
- gfcov, guinea fowl coronavirus
- hcov, human coronavirus
- ibv, infectious bronchitis virus
- m, coronaviral membrane protein
- mers, middle east respiratory syndrome-coronavirus
- mhv, mouse hepatitis virus
- pedv, porcine epidemic diarrhea virus
- pdcov, porcine deltacoronavirus
- phcov, pheasant coronavirus
- phev, porcine hemagglutinating encephalomyelitis virus
- prcov, porcine respiratory coronavirus
- rt-pcr, reverse transcriptase polymerase chain reaction
- s, coronaviral spike protein
- sads-cov, swine acute diarrhea syndrome-coronavirus
- sars-cov, severe acute respiratory syndrome-coronavirus
- sars-cov-2, severe acute respiratory syndrome–coronavirus–2
- tcov, turkey coronavirus
- tgev, transmissible gastroenteritis virus
Collapse
Affiliation(s)
- Alfonso S Gozalo
- Comparative Medicine Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland;,
| | - Tannia S Clark
- Office of Laboratory Animal Medicine, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland
| | - David M Kurtz
- Comparative Medicine Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, Durham, North Carolina
| |
Collapse
|
|
17
|
Pinto MA, da Silva ADS, Rodrigues DDRF, Müller R, de Vasconcelos GALBM, Neves PCDC, de Oliveira JM, Marchevsky RS. Animal models and SARS-CoV-2-induced pulmonary and neurological injuries. Mem Inst Oswaldo Cruz 2023; 117:e220239. [PMID: 36700583 PMCID: PMC9870265 DOI: 10.1590/0074-02760220239] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/10/2022] [Accepted: 11/29/2022] [Indexed: 01/27/2023] Open
Abstract
Laboratory animals are essential mainly for experiments aiming to study pathogenesis and evaluate antivirals and vaccines against emerging human infectious diseases. Preclinical studies of coronavirus disease 19 (COVID-19) pathogenesis have used several animal species as models: transgenic human ACE2 mice (K18 mice), inbred BALB/c or C57BL/6N mice, ferrets, minks, domestic cats and dogs, hamsters, and macaques. However, the choice of an animal model relies on several limitations. Besides the host susceptibility, the researcher's experience with animal model management and the correct interpretation of clinical and laboratory records are crucial to succeed in preclinical translational research. Here, we summarise pathological and clinical findings correlated with virological data and immunological changes observed from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) experimental infections using different well-established SARS-CoV-2 animal model species. This essay aims to critically evaluate the current state of animal model translation to clinical data, as described in the human SARS-CoV-2 infection.
Collapse
Affiliation(s)
- Marcelo Alves Pinto
- Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Desenvolvimento Tecnológico em Virologia, Rio de Janeiro, RJ, Brasil,+ Corresponding author:
| | - Alexandre dos Santos da Silva
- Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Desenvolvimento Tecnológico em Virologia, Rio de Janeiro, RJ, Brasil
| | | | - Rodrigo Müller
- Fundação Oswaldo Cruz-Fiocruz, Instituto de Tecnologia em Imunobiológicos, Laboratório de Experimentação Animal, Rio de Janeiro, RJ, Brasil
| | | | - Patrícia Cristina da Costa Neves
- Fundação Oswaldo Cruz-Fiocruz, Instituto de Tecnologia em Imunobiológicos, Vice-Diretoria de Desenvolvimento Tecnológico, Laboratório de Tecnologia Imunológica, Rio de Janeiro, RJ, Brasil
| | - Jaqueline Mendes de Oliveira
- Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Desenvolvimento Tecnológico em Virologia, Rio de Janeiro, RJ, Brasil
| | - Renato Sergio Marchevsky
- Fundação Oswaldo Cruz-Fiocruz, Instituto de Tecnologia em Imunobiológicos, Laboratório de Neurovirulência, Rio de Janeiro, RJ, Brasil
| |
Collapse
|
|
18
|
Zehr JD, Pond SLK, Millet JK, Olarte-Castillo XA, Lucaci AG, Shank SD, Ceres KM, Choi A, Whittaker GR, Goodman LB, Stanhope MJ. Natural selection differences detected in key protein domains between non-pathogenic and pathogenic Feline Coronavirus phenotypes. BIORXIV : THE PREPRINT SERVER FOR BIOLOGY 2023:2023.01.11.523607. [PMID: 36712007 PMCID: PMC9882035 DOI: 10.1101/2023.01.11.523607] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 01/13/2023]
Abstract
Feline Coronaviruses (FCoVs) commonly cause mild enteric infections in felines worldwide (termed Feline Enteric Coronavirus [FECV]), with around 12% developing into deadly Feline Infectious Peritonitis (FIP; Feline Infectious Peritonitis Virus [FIPV]). Genomic differences between FECV and FIPV have been reported, yet the putative genotypic basis of the highly pathogenic phenotype remains unclear. Here, we used state-of-the-art molecular evolutionary genetic statistical techniques to identify and compare differences in natural selection pressure between FECV and FIPV sequences, as well as to identify FIPV and FECV specific signals of positive selection. We analyzed full length FCoV protein coding genes thought to contain mutations associated with FIPV (Spike, ORF3abc, and ORF7ab). We identified two sites exhibiting differences in natural selection pressure between FECV and FIPV: one within the S1/S2 furin cleavage site, and the other within the fusion domain of Spike. We also found 15 sites subject to positive selection associated with FIPV within Spike, 11 of which have not previously been suggested as possibly relevant to FIP development. These sites fall within Spike protein subdomains that participate in host cell receptor interaction, immune evasion, tropism shifts, host cellular entry, and viral escape. There were 14 sites (12 novel) within Spike under positive selection associated with the FECV phenotype, almost exclusively within the S1/S2 furin cleavage site and adjacent C domain, along with a signal of relaxed selection in FIPV relative to FECV, suggesting that furin cleavage functionality may not be needed for FIPV. Positive selection inferred in ORF7b was associated with the FECV phenotype, and included 24 positively selected sites, while ORF7b had signals of relaxed selection in FIPV. We found evidence of positive selection in ORF3c in FCoV wide analyses, but no specific association with the FIPV or FECV phenotype. We hypothesize that some combination of mutations in FECV may contribute to FIP development, and that is unlikely to be one singular "switch" mutational event. This work expands our understanding of the complexities of FIP development and provides insights into how evolutionary forces may alter pathogenesis in coronavirus genomes.
Collapse
Affiliation(s)
- Jordan D. Zehr
- Department of Biology, Temple University, Institute for Genomics and Evolutionary Medicine, Philadelphia, PA 19122, USA
| | - Sergei L. Kosakovsky Pond
- Department of Biology, Temple University, Institute for Genomics and Evolutionary Medicine, Philadelphia, PA 19122, USA
| | - Jean K. Millet
- Université Paris-Saclay, INRAE, UVSQ, Virologie et Immunologie Moléculaires, 78352 Jouyen-Josas, France
| | - Ximena A. Olarte-Castillo
- Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
- James A. Baker Institute for Animal Health, Cornell University College of Veterinary Medicine, Ithaca, NY, 14853, USA
| | - Alexander G. Lucaci
- Department of Biology, Temple University, Institute for Genomics and Evolutionary Medicine, Philadelphia, PA 19122, USA
| | - Stephen D. Shank
- Department of Biology, Temple University, Institute for Genomics and Evolutionary Medicine, Philadelphia, PA 19122, USA
| | - Kristina M. Ceres
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
| | - Annette Choi
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
- Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
| | - Gary R. Whittaker
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
- Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
| | - Laura B. Goodman
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
- James A. Baker Institute for Animal Health, Cornell University College of Veterinary Medicine, Ithaca, NY, 14853, USA
| | - Michael J. Stanhope
- Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
| |
Collapse
|
|
19
|
He L, Zhong J, Li G, Lin Z, Zhao P, Yang C, Wang H, Zhang Y, Yang X, Wang Z. Development of SARS-CoV-2 animal vaccines using a stable and efficient NDV expression system. J Med Virol 2023; 95:e28237. [PMID: 36258299 PMCID: PMC9874532 DOI: 10.1002/jmv.28237] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/17/2022] [Revised: 09/24/2022] [Accepted: 10/16/2022] [Indexed: 01/27/2023]
Abstract
With the continuation of the coronavirus disease 2019 pandemic and the emergence of new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants, the control of the spread of the virus remains urgent. Various animals, including cats, ferrets, hamsters, nonhuman primates, minks, tree shrews, fruit bats, and rabbits, are susceptible to SARS-CoV-2 infection naturally or experimentally. Therefore, to avoid animals from becoming mixing vessels of the virus, vaccination of animals should be considered. In the present study, we report the establishment of an efficient and stable system using Newcastle disease virus (NDV) as a vector to express SARS-CoV-2 spike protein/subunit for the rapid generation of vaccines against SARS-CoV-2 in animals. Our data showed that the S and S1 protein was sufficiently expressed in rNDV-S and rNDV-S1-infected cells, respectively. The S protein was incorporated into and displayed on the surface of rNDV-S viral particles. Intramuscular immunization with rNDV-S was found to induce the highest level of binding and neutralizing antibodies, as well as strong S-specific T-cell response in mice. Intranasal immunization with rNDV-S1 provoked a robust T-cell response but barely any detectable antibodies. Overall, the NDV-vectored vaccine candidates were able to induce profound humoral and cellular immunity, which will provide a good system for developing vaccines targeting both T-cell and antibody responses.
Collapse
Affiliation(s)
- Lei He
- College of Animal Science and Technology, Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and ControlHenan University of Science and TechnologyHenanLuoyangChina
| | - Jiaying Zhong
- State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouChina
| | - Guichang Li
- State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouChina
| | - Zhengfang Lin
- State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouChina
| | - Peijing Zhao
- Division of MicrobiologyGuangdong Huawei Testing Co., Ltd.GuangzhouChina
| | - Chuhua Yang
- Division of MicrobiologyGuangdong Huawei Testing Co., Ltd.GuangzhouChina
| | - Hairong Wang
- College of Animal Science and Technology, Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and ControlHenan University of Science and TechnologyHenanLuoyangChina
| | - Yuhao Zhang
- College of Animal Science and Technology, Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and ControlHenan University of Science and TechnologyHenanLuoyangChina
| | - Xiaoyun Yang
- State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouChina,Guangzhou LaboratoryGuangzhouChina
| | - Zhongfang Wang
- State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouChina,Guangzhou LaboratoryGuangzhouChina
| |
Collapse
|
|
20
|
Bellinati L, Campalto M, Mazzotta E, Ceglie L, Cavicchio L, Mion M, Lucchese L, Salomoni A, Bortolami A, Quaranta E, Magarotto J, Favarato M, Squarzon L, Natale A. One-Year Surveillance of SARS-CoV-2 Exposure in Stray Cats and Kennel Dogs from Northeastern Italy. Microorganisms 2022; 11:microorganisms11010110. [PMID: 36677401 PMCID: PMC9866628 DOI: 10.3390/microorganisms11010110] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/30/2022] [Revised: 12/27/2022] [Accepted: 12/29/2022] [Indexed: 01/04/2023] Open
Abstract
Dogs and cats are susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). During the pandemic, several studies have been performed on owned cats and dogs, whereas limited data are available on the exposure to stray animals. The objective of this study was to investigate the exposure to SARS-CoV-2 of feral cats and kennel dogs in northeastern Italy, through serological and molecular methods. From May 2021 to September 2022, public health veterinary services collected serum, oropharyngeal, and rectal swab samples from 257 free-roaming dogs newly introduced to shelters, and from 389 feral cats examined during the routinely trap-neutered-return programs. The swabs were analyzed for viral RNA through a real-time reverse transcriptase PCR (rRT-PCR), and sera were tested for the presence of the specific antibody against SARS-CoV-2 (enzyme-linked immunosorbent assay). Serology was positive in nine dogs (9/257) and three cats (3/389), while two asymptomatic cats tested positive to rRT-PCR. One cat turned out to be positive both for serology and molecular analysis. In addition, this study described the case of a possible human-to-animal SARS-CoV-2 transmission in a cat that travelled in close contact to a COVID-19-positive refugee from Ukraine. This study shows that SARS-CoV-2 can infect, in natural conditions, stray cats and kennel dogs in northeastern Italy, although with a low prevalence.
Collapse
Affiliation(s)
- Laura Bellinati
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| | - Mery Campalto
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
- Correspondence:
| | - Elisa Mazzotta
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| | - Letizia Ceglie
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| | - Lara Cavicchio
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| | - Monica Mion
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| | - Laura Lucchese
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| | - Angela Salomoni
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| | - Alessio Bortolami
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| | - Erika Quaranta
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| | | | - Mosè Favarato
- UOSD Genetica e Citogenetica e Diagnostica Molecolare-Azienda ULSS 3 Serenissima, 30174 Venice, Italy
| | - Laura Squarzon
- UOSD Genetica e Citogenetica e Diagnostica Molecolare-Azienda ULSS 3 Serenissima, 30174 Venice, Italy
| | - Alda Natale
- Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
| |
Collapse
|
|
21
|
Imanishi I, Asahina R, Hayashi S, Uchiyama J, Hisasue M, Yamasaki M, Murata Y, Morikawa S, Mizutani T, Sakaguchi M. Guest edited collection serological study of SARS-CoV-2 antibodies in japanese cats using protein-A/G-based ELISA. BMC Vet Res 2022; 18:443. [PMID: 36539820 PMCID: PMC9767852 DOI: 10.1186/s12917-022-03527-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/25/2021] [Accepted: 11/24/2022] [Indexed: 12/24/2022] Open
Abstract
BACKGROUND Little is known about the epidemic status of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in cats in Japan due to insufficiently reliable seroepidemiological analysis methods that are easy to use in cats. RESULTS We developed a protein-A/G-based enzyme-linked immunosorbent assay (ELISA) to detect antibodies against SARS-CoV-2 in cats. The assay was standardized using positive rabbit antibodies against SARS-CoV-2. The ELISA results were consistent with those of a conventional anti-feline-immunoglobulin-G (IgG)-based ELISA. To test the protein-A/G-based ELISA, we collected blood samples from 1,969 cats that had been taken to veterinary clinics in Japan from June to July 2020 and determined the presence of anti-SARS-CoV-2 antibodies. Nine cats were found to have SARS-CoV-2 S1-specific IgG, of which 4 had recombinant receptor-binding domain-specific IgG. Of those 9 samples, one showed neutralizing activity. Based on these findings, we estimated that the prevalence of SARS-CoV-2 neutralizing antibodies in cats in Japan was 0.05% (1/1,969 samples). This prevalence was consistent with the prevalence of neutralizing antibodies against SARS-CoV-2 in humans in Japan according to research conducted at that time. CONCLUSIONS Protein-A/G-based ELISA has the potential to be a standardized method for measuring anti-SARS-CoV-2 antibodies in cats. The infection status of SARS-CoV-2 in cats in Japan might be linked to that in humans.
Collapse
Affiliation(s)
- Ichiro Imanishi
- grid.410786.c0000 0000 9206 2938Department of Microbiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku Sagamihara-shi, Kanagawa, Kanagawa Japan
| | - Ryota Asahina
- grid.258799.80000 0004 0372 2033Faculty of Medicine, Department of Dermatology, Kyoto University, Kyoto, Japan
| | - Shunji Hayashi
- grid.410786.c0000 0000 9206 2938Department of Microbiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku Sagamihara-shi, Kanagawa, Kanagawa Japan
| | - Jumpei Uchiyama
- grid.261356.50000 0001 1302 4472Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan
| | - Masaharu Hisasue
- grid.252643.40000 0001 0029 6233Center for Human and Animal Symbiosis Science, Azabu University, Kanagawa, Japan
| | - Masahiro Yamasaki
- grid.411792.80000 0001 0018 0409Department of Veterinary Internal Medicine, Iwate University, Iwate, Japan
| | - Yoshiteru Murata
- grid.136594.c0000 0001 0689 5974Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, Tokyo, Japan ,Murata Animal Hospital, Chiba, Japan
| | - Shigeru Morikawa
- grid.444568.f0000 0001 0672 2184Faculty of Veterinary Medicine, Department of Microbiology, Okayama University of Science, Ehime, Japan
| | - Tetsuya Mizutani
- grid.136594.c0000 0001 0689 5974Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, Tokyo, Japan
| | | |
Collapse
|
|
22
|
Wang A, Zhu X, Chen Y, Sun Y, Liu H, Ding P, Zhou J, Liu Y, Liang C, Yin J, Zhang G. Serological survey of SARS-CoV-2 in companion animals in China. Front Vet Sci 2022; 9:986619. [PMID: 36532346 PMCID: PMC9748147 DOI: 10.3389/fvets.2022.986619] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/05/2022] [Accepted: 11/14/2022] [Indexed: 08/09/2023] Open
Abstract
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) can be transmitted from human to companion animals. The national wide serological surveillance against SARS-CoV-2 was conducted among pet animals, mainly in cats and dogs, 1 year after the first outbreak of COVID-19 in China. All sera were tested for SARS-CoV-2 IgG antibodies using an indirect enzyme linked immunosorbent assay (ELISA) based on the receptor binding domain (RBD) of spike protein. This late survey takes advantage of the short duration of the serological response in these animals to track recent episode of transmission. A total of 20,592 blood samples were obtained from 25 provinces across 7 geographical regions. The overall seroprevalence of SARS-CoV-2 infections in cats was 0.015% (2/13397; 95% confidence intervals (CI): 0.0, 0.1). The virus infections in cats were only detected in Central (Hubei, 0.375%) and Eastern China (Zhejiang, 0.087%) with a seroprevalence estimated at 0.090 and 0.020%, respectively. In dogs, the seroprevalence of SARS-CoV-2 infections was 0.014% (1/7159; 95% CI: 0.0, 0.1) in the entire nation, seropositive samples were limited to Beijing (0.070%) of Northern China with a prevalence of 0.054%. No seropositive cases were discovered in other geographic regions, nor in other companion animals analyzed in this study. These data reveal the circulation of SARS-CoV-2 in companion animals, although transmission of the virus to domestic cats and dogs is low in China, continuous monitoring is helpful for the better understand of the virus transmission status and the effect on animals.
Collapse
Affiliation(s)
- Aiping Wang
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
| | - Xifang Zhu
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
| | - Yumei Chen
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
- Henan Zhongze Biological Engineering Co., Ltd., Zhengzhou, China
| | - Yaning Sun
- Henan Zhongze Biological Engineering Co., Ltd., Zhengzhou, China
| | - Hongliang Liu
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
- Henan Zhongze Biological Engineering Co., Ltd., Zhengzhou, China
| | - Peiyang Ding
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
- Henan Zhongze Biological Engineering Co., Ltd., Zhengzhou, China
| | - Jingming Zhou
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
| | - Yankai Liu
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
| | - Chao Liang
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
| | - Jiajia Yin
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
| | - Gaiping Zhang
- School of Life Sciences, Zhengzhou University, Zhengzhou, China
| |
Collapse
|
|
23
|
Hu X, Xu B, Xiao Y, Liang S, Zhang C, Song H. Overview and Prospects of Food Biosafety. JOURNAL OF BIOSAFETY AND BIOSECURITY 2022. [DOI: 10.1016/j.jobb.2022.11.001] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022] Open
|
|
24
|
Pappas G, Vokou D, Sainis I, Halley JM. SARS-CoV-2 as a Zooanthroponotic Infection: Spillbacks, Secondary Spillovers, and Their Importance. Microorganisms 2022; 10:2166. [PMID: 36363758 PMCID: PMC9696655 DOI: 10.3390/microorganisms10112166] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/05/2022] [Revised: 10/26/2022] [Accepted: 10/28/2022] [Indexed: 10/06/2023] Open
Abstract
In the midst of a persistent pandemic of a probable zoonotic origin, one needs to constantly evaluate the interplay of SARS-CoV-2 (severe acute respiratory syndrome-related coronavirus-2) with animal populations. Animals can get infected from humans, and certain species, including mink and white-tailed deer, exhibit considerable animal-to-animal transmission resulting in potential endemicity, mutation pressure, and possible secondary spillover to humans. We attempt a comprehensive review of the available data on animal species infected by SARS-CoV-2, as presented in the scientific literature and official reports of relevant organizations. We further evaluate the lessons humans should learn from mink outbreaks, white-tailed deer endemicity, zoo outbreaks, the threat for certain species conservation, the possible implication of rodents in the evolution of novel variants such as Omicron, and the potential role of pets as animal reservoirs of the virus. Finally, we outline the need for a broader approach to the pandemic and epidemics, in general, incorporating the principles of One Health and Planetary Health.
Collapse
Affiliation(s)
- Georgios Pappas
- Institute of Continuing Medical Education of Ioannina, 45333 Ioannina, Greece
| | - Despoina Vokou
- Department of Ecology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
| | - Ioannis Sainis
- Medical School, Faculty of Health Sciences, University of Ioannina, 45110 Ioannina, Greece
| | - John M. Halley
- Laboratory of Ecology, Department of Biological Applications and Technology, Faculty of Health Sciences, University of Ioannina, 45110 Ioannina, Greece
| |
Collapse
|
|
25
|
Evidence of Exposure to SARS-CoV-2 in Dogs and Cats from Households and Animal Shelters in Korea. Animals (Basel) 2022; 12:ani12202786. [PMID: 36290173 PMCID: PMC9597771 DOI: 10.3390/ani12202786] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/25/2022] [Revised: 10/11/2022] [Accepted: 10/13/2022] [Indexed: 11/16/2022] Open
Abstract
The COVID-19 pandemic was caused by the zoonotic SARS-CoV-2. A variety of animals involved in human life worldwide have been investigated for infection. As the degree of infection increased, extensive monitoring in animals became necessary to determine the degree of infection in animals. The study was conducted on a sample of dogs and cats, which were randomly sampled according to the number of confirmed cases in the region. Animals from both COVID-19-confirmed households and generally disease-negative families and animal shelters were included. Tests included real-time qPCR tests for SARS-CoV-2 antigens, ELISA for antibodies, and plaque reduction neutralization tests (PRNT) for neutralizing antibodies. As a result, SARS-CoV-2 viral RNA was detected in 2 cats out of 1018 pets (672 dogs and 346 cats). A total of 16 dogs (2.38%) and 18 cats (5.20%) tested positive using ELISA, and 14 dogs (2.08%) and 17 cats (4.91%) tested positive using PRNT. Antigens of- and/or antibodies to SARS-CoV-2 were detected in the animals regardless of whether the companion family was infected; this was the case even in animal shelters, which have been regarded as relatively safe from transmission. In conclusion, continuous viral circulation between humans and animals is inevitable; therefore, continuous monitoring in animals is required.
Collapse
|
|
26
|
Valleriani F, Jurisic L, Di Pancrazio C, Irelli R, Ciarrocchi E, Martino M, Cocco A, Di Felice E, Colaianni ML, Decaro N, Bonfini B, Lorusso A, Di Teodoro G. A Deletion Encompassing the Furin Cleavage Site in the Spike Encoding Gene Does Not Alter SARS-CoV-2 Replication in Lung Tissues of Mink and Neutralization by Convalescent Human Serum Samples. Pathogens 2022; 11:1152. [PMID: 36297209 PMCID: PMC9609486 DOI: 10.3390/pathogens11101152] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/31/2022] [Revised: 09/26/2022] [Accepted: 10/03/2022] [Indexed: 06/16/2023] Open
Abstract
SARS-CoV-2 has been shown to lose the furin polybasic cleavage site (FCS) following adaptation on cell culture. Deletion occurring in this region, which may include also the FCS flanking regions, seem not to affect virus replication in vitro; however, a chimeric SARS-CoV-2 virus without the sole FCS motif has been associated with lower virulence in mice and lower neutralization values. Moreover, SARS-CoV-2 virus lacking the FCS was shed to lower titers from experimentally infected ferrets and was not transmitted to cohoused sentinel animals, unlike wild-type virus. In this study, we investigated the replication kinetics and cellular tropism of a SARS-CoV-2 isolate carrying a 10-amino acid deletion in the spike protein spanning the FCS in lung ex vivo organ cultures of mink. Furthermore, we tested the neutralization capabilities of human convalescent SARS-CoV-2 positive serum samples against this virus. We showed that this deletion did not significantly hamper neither ex vivo replication nor neutralization activity by convalescent serum samples. This study highlights the importance of the preliminary phenotypic characterization of emerging viruses in ex vivo models and demonstrates that mink lung tissues are permissive to the replication of a mutant form of SARS-CoV-2 showing a deletion spanning the FCS. Notably, we also highlight the need for sequencing viral stocks before any infection study as large deletions may occur leading to the misinterpretation of results.
Collapse
Affiliation(s)
- Fabrizia Valleriani
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| | - Lucija Jurisic
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
- Faculty of Veterinary Medicine, University of Teramo, 64100 Teramo, Italy
| | - Chiara Di Pancrazio
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| | - Roberta Irelli
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| | - Eugenia Ciarrocchi
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| | - Michele Martino
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| | - Antonio Cocco
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| | - Elisabetta Di Felice
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| | | | - Nicola Decaro
- Department of Veterinary Medicine, University of Bari, 70010 Bari, Italy
| | - Barbara Bonfini
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| | - Alessio Lorusso
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| | - Giovanni Di Teodoro
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, 64100 Teramo, Italy
| |
Collapse
|
|
27
|
Thayer V, Gogolski S, Felten S, Hartmann K, Kennedy M, Olah GA. 2022 AAFP/EveryCat Feline Infectious Peritonitis Diagnosis Guidelines. J Feline Med Surg 2022; 24:905-933. [PMID: 36002137 PMCID: PMC10812230 DOI: 10.1177/1098612x221118761] [Citation(s) in RCA: 28] [Impact Index Per Article: 9.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/28/2022]
Abstract
CLINICAL IMPORTANCE Feline infectious peritonitis (FIP) is one of the most important infectious diseases and causes of death in cats; young cats less than 2 years of age are especially vulnerable. FIP is caused by a feline coronavirus (FCoV). It has been estimated that around 0.3% to 1.4% of feline deaths at veterinary institutions are caused by FIP. SCOPE This document has been developed by a Task Force of experts in feline clinical medicine as the 2022 AAFP/EveryCat Feline Infectious Peritonitis Diagnosis Guidelines to provide veterinarians with essential information to aid their ability to recognize cats presenting with FIP. TESTING AND INTERPRETATION Nearly every small animal veterinary practitioner will see cases. FIP can be challenging to diagnose owing to the lack of pathognomonic clinical signs or laboratory changes, especially when no effusion is present. A good understanding of each diagnostic test's sensitivity, specificity, predictive value, likelihood ratio and diagnostic accuracy is important when building a case for FIP. Before proceeding with any diagnostic test or commercial laboratory profile, the clinician should be able to answer the questions of 'why this test?' and 'what do the results mean?' Ultimately, the approach to diagnosing FIP must be tailored to the specific presentation of the individual cat. RELEVANCE Given that the disease is fatal when untreated, the ability to obtain a correct diagnosis is critical. The clinician must consider the individual patient's history, signalment and comprehensive physical examination findings when selecting diagnostic tests and sample types in order to build the index of suspicion 'brick by brick'. Research has demonstrated efficacy of new antivirals in FIP treatment, but these products are not legally available in many countries at this time. The Task Force encourages veterinarians to review the literature and stay informed on clinical trials and new drug approvals.
Collapse
|
|
28
|
Islam A, Ferdous J, Islam S, Sayeed MA, Rahman MK, Saha O, Hassan MM, Shirin T. Transmission dynamics and susceptibility patterns of SARS-CoV-2 in domestic, farmed and wild animals: Sustainable One Health surveillance for conservation and public health to prevent future epidemics and pandemics. Transbound Emerg Dis 2022; 69:2523-2543. [PMID: 34694705 PMCID: PMC8662162 DOI: 10.1111/tbed.14356] [Citation(s) in RCA: 17] [Impact Index Per Article: 5.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/13/2021] [Revised: 10/14/2021] [Accepted: 10/17/2021] [Indexed: 12/11/2022]
Abstract
The exact origin of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and source of introduction into humans has not been established yet, though it might be originated from animals. Therefore, we conducted a study to understand the putative reservoirs, transmission dynamics, and susceptibility patterns of SARS-CoV-2 in animals. Rhinolophus bats are presumed to be natural progenitors of SARS-CoV-2-related viruses. Initially, pangolin was thought to be the source of spillover to humans, but they might be infected by human or other animal species. So, the virus spillover pathways to humans remain unknown. Human-to-animal transmission has been testified in pet, farmed, zoo and free-ranging wild animals. Infected animals can transmit the virus to other animals in natural settings like mink-to-mink and mink-to-cat transmission. Animal-to-human transmission is not a persistent pathway, while mink-to-human transmission continues to be illuminated. Multiple companions and captive wild animals were infected by an emerging alpha variant of concern (B.1.1.7 lineage) whereas Asiatic lions were infected by delta variant, (B.1.617.2). To date, multiple animal species - cat, ferrets, non-human primates, hamsters and bats - showed high susceptibility to SARS-CoV-2 in the experimental condition, while swine, poultry, cattle showed no susceptibility. The founding of SARS-CoV-2 in wild animal reservoirs can confront the control of the virus in humans and might carry a risk to the welfare and conservation of wildlife as well. We suggest vaccinating pets and captive animals to stop spillovers and spillback events. We recommend sustainable One Health surveillance at the animal-human-environmental interface to detect and prevent future epidemics and pandemics by Disease X.
Collapse
Affiliation(s)
- Ariful Islam
- EcoHealth AllianceNew YorkUnited States
- Centre for Integrative Ecology, School of Life and Environmental ScienceDeakin UniversityVictoriaAustralia
- Institute of EpidemiologyDisease Control and Research (IEDCR)DhakaBangladesh
| | - Jinnat Ferdous
- EcoHealth AllianceNew YorkUnited States
- Institute of EpidemiologyDisease Control and Research (IEDCR)DhakaBangladesh
| | - Shariful Islam
- EcoHealth AllianceNew YorkUnited States
- Institute of EpidemiologyDisease Control and Research (IEDCR)DhakaBangladesh
| | - Md. Abu Sayeed
- EcoHealth AllianceNew YorkUnited States
- Institute of EpidemiologyDisease Control and Research (IEDCR)DhakaBangladesh
| | - Md. Kaisar Rahman
- EcoHealth AllianceNew YorkUnited States
- Institute of EpidemiologyDisease Control and Research (IEDCR)DhakaBangladesh
| | - Otun Saha
- EcoHealth AllianceNew YorkUnited States
- Institute of EpidemiologyDisease Control and Research (IEDCR)DhakaBangladesh
- Department of MicrobiologyUniversity of DhakaDhakaBangladesh
| | - Mohammad Mahmudul Hassan
- Faculty of Veterinary MedicineChattogram Veterinary and Animal Sciences UniversityChattogramBangladesh
| | - Tahmina Shirin
- Institute of EpidemiologyDisease Control and Research (IEDCR)DhakaBangladesh
| |
Collapse
|
|
29
|
Review of Method and a New Tool for Decline and Inactive SARS-CoV-2 in Wastewater Treatment. CLEANER CHEMICAL ENGINEERING 2022. [PMCID: PMC9213033 DOI: 10.1016/j.clce.2022.100037] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 12/13/2022]
Abstract
Following the recent outbreak of the COVID-19 pandemic caused by the SARS-CoV-2 virus, monitoring sewage has become crucial, according to reports that the virus was detected in sewage. Currently, various methods are discussed for understanding the SARS-CoV-2 using wastewater surveillance. This paper first introduces the fundamental knowledge of primary, secondary, and tertiary water treatment on SARS-CoV-2. Next, a thorough overview is presented to summarize the recent developments and breakthroughs in removing SARS-CoV-2 using solar water disinfection (SODIS) and UV (UVA (315–400 nm), UVB (280-315 nm), and UVC (100–280 nm)) process. In addition, Due to the fact that the distilled water can be exposed to sunlight if there is no heating source, it can be disinfected using solar water disinfection (SODIS). SODIS, on the other hand, is a well-known method of reducing pathogens in contaminated water; moreover, UVC can inactivate SARS-CoV-2 when the wavelength is between 100 to 280 nanometers. High temperatures (more than 56°C) and UVC are essential for eliminating SARS-CoV-2; however, the SODIS systems use UVA and work at lower temperatures (less than45°C). Therefore, using SODIS methods for wastewater treatment (or providing drinking water) is not appropriate during a situation like the ongoing pandemic. Finally, a wastewater-based epidemiology (WBE) tracking tool for SARS-CoV-2 can be used to detect its presence in wastewater.
Collapse
|
|
30
|
Seroprevalence of SARS-CoV-2 in Client-Owned Cats from Portugal. Vet Sci 2022; 9:vetsci9070363. [PMID: 35878380 PMCID: PMC9315516 DOI: 10.3390/vetsci9070363] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/29/2022] [Revised: 07/13/2022] [Accepted: 07/13/2022] [Indexed: 11/16/2022] Open
Abstract
The close contact between humans and domestic cats raises concerns about the potential risks of SARS-CoV-2 transmission. Thus, this study aims to investigate anti-SARS-CoV-2 seroprevalence in client-owned cats from Portugal and evaluate the infection risk of cats that maintain contact with human COVID-19 cases. A total of 176 cats, belonging to 94 households, were sampled. Cat owners answered an online questionnaire, and cats were screened for antibodies against SARS-CoV-2 using a commercial ELISA. Twenty (21.3%) households reported at least one confirmed human COVID-19 case. Forty cats (22.7%) belonged to a COVID-19-positive and 136 (77.3%) to a COVID-19-negative household. The seroprevalences of cats from COVID-19-positive and -negative households were 5.0% (2/40) and 0.7% (1/136). The two SARS-CoV-2-seropositive cats from COVID-19-positive households had an indoor lifestyle, and their owners stated that they maintained a close and frequent contact with them, even after being diagnosed with COVID-19, pointing towards human-to-cat transmission. The SARS-CoV-2-seropositive cat from the COVID-19-negative household had a mixed indoor/outdoor lifestyle and chronic diseases. Owners of the three SARS-CoV-2-seropositive cats did not notice clinical signs or behavior changes. This study highlights the low risk of SARS-CoV-2 transmission from COVID-19-positive human household members to domestic cats, even in a context of close and frequent human–animal contact.
Collapse
|
|
31
|
Murphy H, Sanchez S, Ahmed S, Rhaman MM, Di D, Dileepan M, Heinrich D, Liang Y, Ly H. SARS-CoV-2 in companion animals: Do levels of SARS-CoV-2 seroconversion in pets correlate with those of pet's owners and with protection against subsequent SARS-CoV-2 infection? Virulence 2022; 13:1216-1220. [PMID: 35799426 PMCID: PMC9345533 DOI: 10.1080/21505594.2022.2098922] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/27/2022] Open
Affiliation(s)
| | | | | | | | - Da Di
- Department of Veterinary & Biomedical Sciences
| | | | - Daniel Heinrich
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, Twin Cities, USA
| | | | - Hinh Ly
- Department of Veterinary & Biomedical Sciences
| |
Collapse
|
|
32
|
Stumpf MM, Freeman B, Mills L, Lester S, Chu VT, Kirking HL, Thornburg NJ, Killerby ME. Examination of Common Coronavirus Antibodies in SARS-CoV-2-Infected and Uninfected Participants in a Household Transmission Investigation. Open Forum Infect Dis 2022; 9:ofac212. [PMID: 35873297 PMCID: PMC9297157 DOI: 10.1093/ofid/ofac212] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/02/2021] [Accepted: 04/21/2022] [Indexed: 11/12/2022] Open
Abstract
We compared paired serum specimens from household contacts of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases with detectable SARS-CoV-2 seroconversion with contacts who remained seronegative. No protection from SARS-CoV-2 infection was associated with human coronavirus antibodies; however, an increase in common betacoronavirus antibodies was associated with seroconversion to SARS-CoV-2 in mild to moderately ill cases.
Collapse
Affiliation(s)
- Megan M Stumpf
- Centers for Disease Control and Prevention, Atlanta, Georgia, USA
| | - Brandi Freeman
- Centers for Disease Control and Prevention, Atlanta, Georgia, USA
| | - Lisa Mills
- Centers for Disease Control and Prevention, Atlanta, Georgia, USA
| | - Sandra Lester
- Centers for Disease Control and Prevention, Atlanta, Georgia, USA
| | - Victoria T Chu
- Centers for Disease Control and Prevention, Atlanta, Georgia, USA
| | - Hannah L Kirking
- Centers for Disease Control and Prevention, Atlanta, Georgia, USA
| | | | - Marie E Killerby
- Centers for Disease Control and Prevention, Atlanta, Georgia, USA
| |
Collapse
|
|
33
|
Gao YY, Liang XY, Wang Q, Zhang S, Zhao H, Wang K, Hu GX, Liu WJ, Gao FS. Mind the feline coronavirus: Comparison with SARS-CoV-2. Gene 2022; 825:146443. [PMID: 35337854 PMCID: PMC8938304 DOI: 10.1016/j.gene.2022.146443] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/29/2021] [Revised: 02/25/2022] [Accepted: 03/18/2022] [Indexed: 12/13/2022]
Abstract
Both feline coronavirus (FCoV) and SARS-CoV-2 are coronaviruses that infect cats and humans, respectively. However, cats have been shown to be susceptible to SARS-CoV-2, and FCoV also had been shown to infect human. To elucidate the relationship between FCoV and SARS-CoV-2, we highlight the main characteristics of the genome, the receptor usage, and the correlation of the receptor-binding domain (RBD) of spike proteins in FCoV and SARS-CoV-2. It is demonstrated that FCoV and SARS-CoV-2 are closely related to the main characteristics of the genome, receptor usage, and RBD of spike proteins with similar furin cleavage sites. In particular, the affinity of the conserved feline angiotensin-converting enzyme 2 (fACE2) receptor to the RBD of SARS-CoV-2 suggests that cats are susceptible to SARS-CoV-2. In addition, cross-species of coronaviruses between cats and humans or other domesticated animals are also discussed. This review sheds light on cats as potential intermediate hosts for SARS-CoV-2 transmission, and cross-species transmission or zoonotic infection of FCoV and SARS-CoV-2 between cats and humans was identified.
Collapse
Affiliation(s)
- Yong-Yu Gao
- College of Animal Medicine, Jilin Agricultural University, Changchun 130118, China
| | - Xiang-Yu Liang
- College of Animal Medicine, Jilin Agricultural University, Changchun 130118, China
| | - Qian Wang
- Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130117, China
| | - Shuang Zhang
- College of Animal Medicine, Jilin Agricultural University, Changchun 130118, China
| | - Han Zhao
- College of Animal Medicine, Jilin Agricultural University, Changchun 130118, China
| | - Kai Wang
- College of Animal Medicine, Jilin Agricultural University, Changchun 130118, China.
| | - Gui-Xue Hu
- College of Animal Medicine, Jilin Agricultural University, Changchun 130118, China.
| | - William J Liu
- NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
| | - Feng-Shan Gao
- College of Life Science and Technology, Dalian University, Dalian 116622, China.
| |
Collapse
|
|
34
|
Doliff R, Martens P. Cats and SARS-CoV-2: A Scoping Review. Animals (Basel) 2022; 12:1413. [PMID: 35681877 PMCID: PMC9179433 DOI: 10.3390/ani12111413] [Citation(s) in RCA: 21] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/20/2022] [Revised: 05/20/2022] [Accepted: 05/23/2022] [Indexed: 11/22/2022] Open
Abstract
Since the beginning of the COVID-19 pandemic, various animal species were found to be susceptible to SARS-CoV-2 infection. The close contact that exists between humans and cats warrants special attention to the role of this species. Therefore, a scoping review was performed to obtain a comprehensive overview of the existing literature, and to map key concepts, types of research, and possible gaps in the research. A systematic search of the databases PubMed, Google Scholar, and Scopus and the preprint servers medRxiv and bioRxiv was performed. After a two-step screening process, 27 peer-reviewed articles, 8 scientific communication items, and 2 unpublished pre-prints were included. The main themes discussed were susceptibility to SARS-CoV-2, induced immunity, prevalence of infection, manifestation of infection, interspecies transmission between humans and cats, and lastly, intraspecies transmission between cats. The main gaps in the research identified were a lack of large-scale studies, underrepresentation of stray, feral, and shelter cat populations, lack of investigation into cat-to-cat transmissions under non-experimental conditions, and the relation of cats to other animal species regarding SARS-CoV-2. Overall, cats seemingly play a limited role in the spread of SARS-CoV-2. While cats are susceptible to the virus and reverse zoonotic transmission from humans to cats happens regularly, there is currently no evidence of SARS-CoV-2 circulation among cats.
Collapse
Affiliation(s)
| | - Pim Martens
- University College Venlo, Maastricht University, Nassaustraat 36, 5911 BV Venlo, The Netherlands;
| |
Collapse
|
|
35
|
Villanueva‐Saz S, Giner J, Tobajas AP, Pérez MD, González‐Ramírez AM, Macías‐León J, González A, Verde M, Yzuel A, Hurtado‐Guerrero R, Pardo J, Santiago L, Paño‐Pardo JR, Ruíz H, Lacasta DM, Sánchez L, Marteles D, Gracia AP, Fernández A. Serological evidence of SARS-CoV-2 and co-infections in stray cats in Spain. Transbound Emerg Dis 2022; 69:1056-1064. [PMID: 33686768 PMCID: PMC8250530 DOI: 10.1111/tbed.14062] [Citation(s) in RCA: 61] [Impact Index Per Article: 20.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/22/2020] [Revised: 02/09/2021] [Accepted: 03/05/2021] [Indexed: 12/12/2022]
Abstract
A new coronavirus known as SARS-CoV-2 emerged in Wuhan in 2019 and spread rapidly to the rest of the world causing the pandemic disease named coronavirus disease of 2019 (COVID-19). Little information is known about the impact this virus can cause upon domestic and stray animals. The potential impact of SARS-CoV-2 has become of great interest in cats due to transmission among domestic cats and the severe phenotypes described recently in a domestic cat. In this context, there is a public health warning that needs to be investigated in relation with the epidemiological role of this virus in stray cats. Consequently, in order to know the impact of the possible transmission chain, blood samples were obtained from 114 stray cats in the city of Zaragoza (Spain) and tested for SARS-CoV-2 and other selected pathogens susceptible to immunosuppression including Toxoplasma gondii, Leishmania infantum, feline leukaemia virus (FeLV) and feline immunodeficiency virus (FIV) from January to October 2020. Four cats (3.51%), based on enzyme-linked immunosorbent assay (ELISA) using the receptor binding domain (RBD) of Spike antigen, were seroreactive to SARS-CoV-2. T. gondii, L. infantum, FeLV and FIV seroprevalence was 12.28%, 16.67%, 4.39% and 19.30%, respectively. Among seropositive cats to SARS-CoV-2, three cats were also seropositive to other pathogens including antibodies detected against T. gondii and FIV (n = 1); T. gondii (n = 1); and FIV and L. infantum (n = 1). The subjects giving positive for SARS-CoV-2 were captured in urban areas of the city in different months: January 2020 (2/4), February 2020 (1/4) and July 2020 (1/4). This study revealed, for the first time, the exposure of stray cats to SARS-CoV-2 in Spain and the existence of concomitant infections with other pathogens including T. gondii, L. infantum and FIV, suggesting that immunosuppressed animals might be especially susceptible to SARS-CoV-2 infection.
Collapse
Affiliation(s)
- Sergio Villanueva‐Saz
- Clinical Immunology Laboratory, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
- Department of Pharmacology and PhysiologyVeterinary FacultyUniversity of ZaragozaZaragozaSpain
- Instituto Agroalimentario de Aragón‐IA2 (Universidad de Zaragoza‐CITA)ZaragozaSpain
| | - Jacobo Giner
- Clinical Immunology Laboratory, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
- Deparment of Animal Pathology, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Ana Pilar Tobajas
- Instituto Agroalimentario de Aragón‐IA2 (Universidad de Zaragoza‐CITA)ZaragozaSpain
- Department of Animal Production and Sciences of the Food, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - María Dolores Pérez
- Instituto Agroalimentario de Aragón‐IA2 (Universidad de Zaragoza‐CITA)ZaragozaSpain
- Department of Animal Production and Sciences of the Food, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Andrés Manuel González‐Ramírez
- Institute for Biocomputation and Physics of Complex Systems (BIFI)Edificio I+DCampus Rio EbroUniversity of ZaragozaZaragozaSpain
| | - Javier Macías‐León
- Instituto Agroalimentario de Aragón‐IA2 (Universidad de Zaragoza‐CITA)ZaragozaSpain
| | - Ana González
- Deparment of Animal Pathology, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Maite Verde
- Clinical Immunology Laboratory, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
- Instituto Agroalimentario de Aragón‐IA2 (Universidad de Zaragoza‐CITA)ZaragozaSpain
- Deparment of Animal Pathology, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Andrés Yzuel
- Clinical Immunology Laboratory, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Ramón Hurtado‐Guerrero
- Institute for Biocomputation and Physics of Complex Systems (BIFI)Edificio I+DCampus Rio EbroUniversity of ZaragozaZaragozaSpain
- Aragon I+D Foundation (ARAID)ZaragozaSpain
- Laboratorio de Microscopías Avanzada (LMA)Edificio I+D, Campus Rio EbroUniversity of ZaragozaZaragozaSpain
- Copenhagen Center for GlycomicsCopenhagenDenmark
- Department of Cellular and Molecular MedicineSchool of DentistryUniversity of CopenhagenCopenhagenDenmark
| | - Julián Pardo
- Aragon I+D Foundation (ARAID)ZaragozaSpain
- Aragon Health Research Institute (IIS Aragón)ZaragozaSpain
- Department of MicrobiologyPediatrics, Radiology and Public HealthZaragoza University of ZaragozaZaragozaSpain
| | | | - José Ramón Paño‐Pardo
- Aragon Health Research Institute (IIS Aragón)ZaragozaSpain
- Infectious Disease DepartmentUniversity Hospital Lozano BlesaZaragozaSpain
| | - Héctor Ruíz
- Deparment of Animal Pathology, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Delia María Lacasta
- Instituto Agroalimentario de Aragón‐IA2 (Universidad de Zaragoza‐CITA)ZaragozaSpain
- Deparment of Animal Pathology, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Lourdes Sánchez
- Instituto Agroalimentario de Aragón‐IA2 (Universidad de Zaragoza‐CITA)ZaragozaSpain
- Department of Animal Production and Sciences of the Food, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Diana Marteles
- Clinical Immunology Laboratory, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Ana Pilar Gracia
- Instituto Agroalimentario de Aragón‐IA2 (Universidad de Zaragoza‐CITA)ZaragozaSpain
- Department of Animal Production and Sciences of the Food, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| | - Antonio Fernández
- Clinical Immunology Laboratory, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
- Instituto Agroalimentario de Aragón‐IA2 (Universidad de Zaragoza‐CITA)ZaragozaSpain
- Deparment of Animal Pathology, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
| |
Collapse
|
|
36
|
Hoyte A, Webster M, Ameiss K, Conlee DA, Hainer N, Hutchinson K, Burakova Y, Dominowski PJ, Baima ET, King VL, Rosey EL, Hardham JM, Millership J, Kumar M. Experimental veterinary SARS-CoV-2 vaccine cross neutralization of the Delta (B.1.617.2) variant virus in cats. Vet Microbiol 2022; 268:109395. [PMID: 35339817 PMCID: PMC8915440 DOI: 10.1016/j.vetmic.2022.109395] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/02/2021] [Revised: 03/04/2022] [Accepted: 03/06/2022] [Indexed: 10/25/2022]
Abstract
SARS-CoV-2 has exhibited varying pathogenesis in a variety of Mammalia family's including Canidae, Mustelidae, Hominidae, Cervidae, Hyaenidae, and Felidae. Novel SARS-CoV-2 variants characterized by spike protein mutations have recently resulted in clinical and epidemiological concerns, as they potentially have increased infectious rates, increased transmission, or reduced neutralization by antibodies produced via vaccination. Many variants have been identified at this time, but the variant of continuing concern has been the Delta variant (B.1.617.2), due to its increased transmissibility and infectious rate. Felines vaccinated using an experimental SARS-CoV-2 spike protein-based veterinary vaccine mounted a robust immune response to the SARS-CoV-2 spike protein. Using a reporter virus particle system and feline serum, we have verified that vaccinated felines produce antibodies that neutralize the SARS-CoV-2 Wuhan strain and variant B.1.617.2 at comparable levels.
Collapse
|
|
37
|
Lebedin Y, Petukhov P, Maygurova V, Klyuchnikova P, Naidenko S. Occurrence of Spike Antigen Specific SARS-CoV-2 Antibodies in Pre-Pandemic Samples of Domestic Cats Raises New Questions. BIOL BULL+ 2022. [DOI: 10.1134/s1062359021150103] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
|
|
38
|
Mastutik G, Rohman A, I'tishom R, Ruiz-Arrondo I, de Blas I. Experimental and natural infections of severe acute respiratory syndrome-related coronavirus 2 in pets and wild and farm animals. Vet World 2022; 15:565-589. [PMID: 35497948 PMCID: PMC9047133 DOI: 10.14202/vetworld.2022.565-589] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/19/2021] [Accepted: 01/25/2022] [Indexed: 12/27/2022] Open
Abstract
The severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has spread globally and has led to extremely high mortality rates. In addition to infecting humans, this virus also has infected animals. Experimental studies and natural infections showed that dogs have a low susceptibility to SARS-CoV-2 infection, whereas domesticated cats and other animals in the family Felidae, such as lions, tigers, snow leopards, and cougars, have a high susceptibility to viral infections. In addition, wild white-tailed deer, gorillas, and otters have been found to be infected by SARS-CoV-2. Furry farm animals, such as minks, have a high susceptibility to SARS-CoV-2 infection. The virus appears to spread among minks and generate several new mutations, resulting in increased viral virulence. Furthermore, livestock animals, such as cattle, sheep, and pigs, were found to have low susceptibility to the virus, whereas chicken, ducks, turkeys, quail, and geese did not show susceptibility to SARS-CoV-2 infection. This knowledge can provide insights for the development of SARS-CoV-2 mitigation strategies in animals and humans. Therefore, this review focuses on experimental (both replication and transmission) in vitro, ex vivo, and in vivo studies of SARS-CoV-2 infections in pets and in wild and farm animals, and to provide details on the mechanism associated with natural infection.
Collapse
Affiliation(s)
- Gondo Mastutik
- Department of Anatomic Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya 60131, Indonesia
| | - Ali Rohman
- Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia
| | - Reny I'tishom
- Department of Medical Biology, Faculty of Medicine, Universitas Airlangga, Surabaya 60131, Indonesia
| | - Ignacio Ruiz-Arrondo
- Center for Rickettsioses and Arthropod-Borne Diseases, Hospital Universitario San Pedro–CIBIR, Logroño, Spain
| | - Ignacio de Blas
- Department of Animal Pathology, Faculty of Veterinary Sciences, Instituto Universitario de Investigación Mixto Agroalimentario de Aragón (IA2), Universidad de Zaragoza, Spain
| |
Collapse
|
|
39
|
Lu J, Chen SA, Khan MB, Brassard R, Arutyunova E, Lamer T, Vuong W, Fischer C, Young HS, Vederas JC, Lemieux MJ. Crystallization of Feline Coronavirus M pro With GC376 Reveals Mechanism of Inhibition. Front Chem 2022; 10:852210. [PMID: 35281564 PMCID: PMC8907848 DOI: 10.3389/fchem.2022.852210] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/11/2022] [Accepted: 02/01/2022] [Indexed: 12/28/2022] Open
Abstract
Coronaviruses infect a variety of hosts in the animal kingdom, and while each virus is taxonomically different, they all infect their host via the same mechanism. The coronavirus main protease (Mpro, also called 3CLpro), is an attractive target for drug development due to its essential role in mediating viral replication and transcription. An Mpro inhibitor, GC376, has been shown to treat feline infectious peritonitis (FIP), a fatal infection in cats caused by internal mutations in the feline enteric coronavirus (FECV). Recently, our lab demonstrated that the feline drug, GC373, and prodrug, GC376, are potent inhibitors of SARS-CoV-2 Mpro and solved the structures in complex with the drugs; however, no crystal structures of the FIP virus (FIPV) Mpro with the feline drugs have been published so far. Here, we present crystal structures of FIPV Mpro-GC373/GC376 complexes, revealing the inhibitors covalently bound to Cys144 in the active site, similar to SARS-CoV-2 Mpro. Additionally, GC376 has a higher affinity for FIPV Mpro with lower nanomolar Ki values compared to SARS-CoV and SARS-CoV-2 Mpro. We also show that improved derivatives of GC376 have higher potency for FIPV Mpro. Since GC373 and GC376 represent strong starting points for structure-guided drug design, determining the crystal structures of FIPV Mpro with these inhibitors are important steps in drug optimization and structure-based broad-spectrum antiviral drug discovery.
Collapse
Affiliation(s)
- Jimmy Lu
- Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
- Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada
| | - Sizhu Amelia Chen
- Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
- Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada
| | | | - Raelynn Brassard
- Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
- Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada
| | - Elena Arutyunova
- Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
- Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada
| | - Tess Lamer
- Department of Chemistry, University of Alberta, Edmonton, AB, Canada
| | - Wayne Vuong
- Department of Chemistry, University of Alberta, Edmonton, AB, Canada
| | - Conrad Fischer
- Department of Chemistry, University of Alberta, Edmonton, AB, Canada
| | - Howard S. Young
- Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
| | - John C. Vederas
- Department of Chemistry, University of Alberta, Edmonton, AB, Canada
| | - M. Joanne Lemieux
- Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
- Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada
| |
Collapse
|
|
40
|
Zou Y, Cao X, Yang B, Deng L, Xu Y, Dong S, Li W, Wu C, Cao G. In Silico Infection Analysis (iSFA) Identified Coronavirus Infection and Potential Transmission Risk in Mammals. Front Mol Biosci 2022; 9:831876. [PMID: 35211513 PMCID: PMC8861533 DOI: 10.3389/fmolb.2022.831876] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/09/2021] [Accepted: 01/04/2022] [Indexed: 11/26/2022] Open
Abstract
Coronaviruses are a great source of threat to public health which could infect various species and cause diverse diseases. However, the epidemic’s spreading among different species remains elusive. This study proposed an in silico infection analysis (iSFA) system that includes pathogen genome or transcript mining in transcriptome data of the potential host and performed a comprehensive analysis about the infection of 38 coronaviruses in wild animals, based on 2,257 transcriptome datasets from 89 mammals’ lung and intestine, and revealed multiple potential coronavirus infections including porcine epidemic diarrhea virus (PEDV) infection in Equus burchellii. Then, through our transmission network analysis, potential intermediate hosts of five coronaviruses were identified. Notably, iSFA results suggested that the expression of coronavirus receptor genes tended to be downregulated after infection by another virus. Finally, binding affinity and interactive interface analysis of S1 protein and ACE2 from different species demonstrated the potential inter-species transmission barrier and cross-species transmission of SARS-CoV-2. Meanwhile, the iSFA system developed in this study could be further applied to conduct the source tracing and host prediction of other pathogen-induced diseases, thus contributing to the epidemic prevention and control.
Collapse
Affiliation(s)
- Yanyan Zou
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- College of Informatics, Huazhong Agricultural University, Wuhan, China
| | - Xiaojian Cao
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
| | - Bing Yang
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
| | - Lulu Deng
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
| | - Yangyang Xu
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
| | - Shuang Dong
- Department of Medical Oncology, Hubei Cancer Hospital, Huazhong University of Science and Technology, Wuhan, China
| | - Wentao Li
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
| | - Chengchao Wu
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- *Correspondence: Chengchao Wu, ; Gang Cao,
| | - Gang Cao
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Bio-Medical Center, Huazhong Agricultural University, Wuhan, China
- *Correspondence: Chengchao Wu, ; Gang Cao,
| |
Collapse
|
|
41
|
Korath ADJ, Janda J, Untersmayr E, Sokolowska M, Feleszko W, Agache I, Adel Seida A, Hartmann K, Jensen‐Jarolim E, Pali‐Schöll I. One Health: EAACI Position Paper on coronaviruses at the human-animal interface, with a specific focus on comparative and zoonotic aspects of SARS-CoV-2. Allergy 2022; 77:55-71. [PMID: 34180546 PMCID: PMC8441637 DOI: 10.1111/all.14991] [Citation(s) in RCA: 16] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/13/2021] [Accepted: 06/24/2021] [Indexed: 12/15/2022]
Abstract
The latest outbreak of a coronavirus disease in 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), evolved into a worldwide pandemic with massive effects on health, quality of life, and economy. Given the short period of time since the outbreak, there are several knowledge gaps on the comparative and zoonotic aspects of this new virus. Within the One Health concept, the current EAACI position paper dwells into the current knowledge on SARS-CoV-2's receptors, symptoms, transmission routes for human and animals living in close vicinity to each other, usefulness of animal models to study this disease and management options to avoid intra- and interspecies transmission. Similar pandemics might appear unexpectedly and more frequently in the near future due to climate change, consumption of exotic foods and drinks, globe-trotter travel possibilities, the growing world population, the decreasing production space, declining room for wildlife and free-ranging animals, and the changed lifestyle including living very close to animals. Therefore, both the society and the health authorities need to be aware and well prepared for similar future situations, and research needs to focus on prevention and fast development of treatment options (medications, vaccines).
Collapse
Affiliation(s)
- Anna D. J. Korath
- Comparative MedicineInteruniversity Messerli Research InstituteUniversity of Veterinary Medicine and Medical University ViennaViennaAustria
| | - Jozef Janda
- Faculty of ScienceCharles UniversityPragueCzech Republic
| | - Eva Untersmayr
- Institute of Pathophysiology and Allergy ResearchCenter of Pathophysiology, Infectiology and ImmunologyMedical University of ViennaViennaAustria
| | - Milena Sokolowska
- Swiss Institute of Allergy and Asthma Research (SIAF),University of ZurichZurichSwitzerland
| | - Wojciech Feleszko
- Department of Paediatric Allergy and PulmonologyThe Medical University of WarsawWarsawPoland
| | | | - Ahmed Adel Seida
- Department of Microbiology and ImmunologyFaculty of Veterinary MedicineCairo UniversityCairoEgypt
| | - Katrin Hartmann
- Medizinische KleintierklinikZentrum für Klinische TiermedizinLMUMunichGermany
| | - Erika Jensen‐Jarolim
- Comparative MedicineInteruniversity Messerli Research InstituteUniversity of Veterinary Medicine and Medical University ViennaViennaAustria
- Institute of Pathophysiology and Allergy ResearchCenter of Pathophysiology, Infectiology and ImmunologyMedical University of ViennaViennaAustria
| | - Isabella Pali‐Schöll
- Comparative MedicineInteruniversity Messerli Research InstituteUniversity of Veterinary Medicine and Medical University ViennaViennaAustria
- Institute of Pathophysiology and Allergy ResearchCenter of Pathophysiology, Infectiology and ImmunologyMedical University of ViennaViennaAustria
| |
Collapse
|
|
42
|
Frazzini S, Amadori M, Turin L, Riva F. SARS CoV-2 infections in animals, two years into the pandemic. Arch Virol 2022; 167:2503-2517. [PMID: 36207554 PMCID: PMC9543933 DOI: 10.1007/s00705-022-05609-1] [Citation(s) in RCA: 23] [Impact Index Per Article: 7.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/31/2022] [Accepted: 08/19/2022] [Indexed: 12/14/2022]
Abstract
In December 2019, several cases of pneumonia caused by a novel coronavirus, later identified as SARS-CoV-2, were detected in the Chinese city of Wuhan. Due to its rapid worldwide spread, on 11 March 2020 the World Health Organization declared a pandemic state. Since this new virus is genetically similar to the coronaviruses of bats, SARS-CoV-2 was hypothesized to have a zoonotic origin. Within a year of the appearance of SARS-CoV-2, several cases of infection were also reported in animals, suggesting human-to-animal and animal-to-animal transmission among mammals. Natural infection has been found in companion animals as well as captive animals such as lions, tigers, and gorillas. Among farm animals, so far, minks have been found to be susceptible to SARS-CoV-2 infection, whereas not all the relevant studies agree on the susceptibility of pigs. Experimental infections have documented the susceptibility to SARS-CoV-2 of further animal species, including mice, hamsters, cats, dogs, ferrets, raccoon dogs, cattle, and non-human primates. Experimental infections have proven crucial for clarifying the role of animals in transmission and developing models for viral pathogenesis and immunotherapy. On the whole, this review aims to update and critically revise the current information on natural and experimental SARS-CoV-2 infections in animals.
Collapse
Affiliation(s)
- Sara Frazzini
- Department of Veterinary Medicine (DIMEVET), University of Milan, Milan, Italy
| | | | - Lauretta Turin
- Department of Veterinary Medicine (DIMEVET), University of Milan, Milan, Italy
| | - Federica Riva
- Department of Veterinary Medicine (DIMEVET), University of Milan, Milan, Italy
| |
Collapse
|
|
43
|
DEMİRCİ M, YIĞIN A, KOCAZEYBEK B, KESKİN O. COVID-19 infections in pets such as cats and dogs. MEHMET AKIF ERSOY ÜNIVERSITESI VETERINER FAKÜLTESI DERGISI 2021. [DOI: 10.24880/maeuvfd.953646] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/09/2022] Open
|
|
44
|
Hassanin AA, Haidar Abbas Raza S, Ahmed Ujjan J, Aysh ALrashidi A, Sitohy BM, AL-surhanee AA, Saad AM, Mohamed Al -Hazani T, Osman Atallah O, Al Syaad KM, Ezzat Ahmed A, Swelum AA, El-Saadony MT, Sitohy MZ. Emergence, evolution, and vaccine production approaches of SARS-CoV-2 virus: Benefits of getting vaccinated and common questions. Saudi J Biol Sci 2021; 29:1981-1997. [PMID: 34924802 PMCID: PMC8667566 DOI: 10.1016/j.sjbs.2021.12.020] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/09/2021] [Revised: 11/30/2021] [Accepted: 12/09/2021] [Indexed: 12/23/2022] Open
Abstract
The emergence of coronavirus disease 2019 (COVID-19) pandemic in Wuhan city, China at the end of 2019 made it urgent to identify the origin of the causal pathogen and its molecular evolution, to appropriately design an effective vaccine. This study analyzes the evolutionary background of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 or SARS-2) in accordance with its close relative SARS-CoV (SARS-1), which was emerged in 2002. A comparative genomic and proteomic study was conducted on SARS-2, SARS-1, and Middle East respiratory syndrome coronavirus (MERS), which was emerged in 2012. In silico analysis inferred the genetic variability among the tested viruses. The SARS-1 genome harbored 11 genes encoding 12 proteins, while SARS-2 genome contained only 10 genes encoding for 10 proteins. MERS genome contained 11 genes encoding 11 proteins. The analysis also revealed a slight variation in the whole genome size of SARS-2 comparing to its siblings resulting from sequential insertions and deletions (indels) throughout the viral genome particularly ORF1AB, spike, ORF10 and ORF8. The effective indels were observed in the gene encoding the spike protein that is responsible for viral attachment to the angiotensin-converting enzyme 2 (ACE2) cell receptor and initiating infection. These indels are responsible for the newly emerging COVID-19 variants αCoV, βCoV, γCoV and δCoV. Nowadays, few effective COVID-19 vaccines developed based on spike (S) glycoprotein were approved and become available worldwide. Currently available vaccines can relatively prevent the spread of COVID-19 and suppress the disease. The traditional (killed or attenuated virus vaccine and antibody-based vaccine) and innovated vaccine production technologies (RNA- and DNA-based vaccines and viral vectors) are summarized in this review. We finally highlight the most common questions related to COVID-19 disease and the benefits of getting vaccinated.
Collapse
|
|
45
|
Dileepan M, Di D, Huang Q, Ahmed S, Heinrich D, Ly H, Liang Y. Seroprevalence of SARS-CoV-2 (COVID-19) exposure in pet cats and dogs in Minnesota, USA. Virulence 2021; 12:1597-1609. [PMID: 34125647 PMCID: PMC8205054 DOI: 10.1080/21505594.2021.1936433] [Citation(s) in RCA: 57] [Impact Index Per Article: 14.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/28/2021] [Revised: 05/20/2021] [Accepted: 05/24/2021] [Indexed: 01/13/2023] Open
Abstract
The COVID-19 pandemic caused by the coronavirus SARS-CoV-2 is continuing to spread globally. SARS-CoV-2 infections of feline and canine species have also been reported. However, it is not entirely clear to what extent natural SARS-CoV-2 infection of pet dogs and cats is in households. We have developed enzyme-linked immunosorbent assays (ELISAs) using recombinant SARS-CoV-2 nucleocapsid (N) protein and the receptor-binding-domain (RBD) of the spike protein, and the SARS-CoV-2 spike-pseudotyped vesicular stomatitis virus (VSV)-based neutralization assay to screen serum samples of 239 pet cats and 510 pet dogs in Minnesota in the early phase of the COVID-19 pandemic from mid-April to early June 2020 for evidence of SARS-CoV-2 exposures. A cutoff value was used to identify the seropositive samples in each experiment. The average seroprevalence of N- and RBD-specific antibodies in pet cats were 8% and 3%, respectively. Among nineteen (19) N-seropositive cat sera, fifteen (15) exhibited neutralizing activity and seven (7) were also RBD-seropositive. The N-based ELISA is also specific and does not cross react with antigens of common feline coronaviruses. In contrast, SARS-CoV-2 antibodies were detected at a very low percentage in pet dogs (~ 1%) and were limited to IgG antibodies against SARS-CoV-2 N protein with no neutralizing activities. Our results demonstrate that SARS-CoV-2 seropositive rates are higher in pet cats than in pet dogs in MN early in the pandemic and that SARS-CoV-2 N-specific IgG antibodies can detect SARS-CoV-2 infections in companion animals with higher levels of specificity and sensitivity than RBD-specific IgG antibodies in ELISA-based assays.
Collapse
Affiliation(s)
- Mythili Dileepan
- Department of Veterinary & Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Twin Cities, USA
| | - Da Di
- Department of Veterinary & Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Twin Cities, USA
| | - Qinfeng Huang
- Department of Veterinary & Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Twin Cities, USA
| | - Shamim Ahmed
- Department of Veterinary & Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Twin Cities, USA
| | - Daniel Heinrich
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, Twin Cities, USA
| | - Hinh Ly
- Department of Veterinary & Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Twin Cities, USA
| | - Yuying Liang
- Department of Veterinary & Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Twin Cities, USA
| |
Collapse
|
|
46
|
Yilmaz A, Kayar A, Turan N, Iskefli O, Bayrakal A, Roman-Sosa G, Or E, Tali HE, Kocazeybek B, Karaali R, Bold D, Sadeyen JR, Lukosaityte D, Chang P, Iqbal M, Richt JA, Yilmaz H. Presence of Antibodies to SARS-CoV-2 in Domestic Cats in Istanbul, Turkey, Before and After COVID-19 Pandemic. Front Vet Sci 2021; 8:707368. [PMID: 34712718 PMCID: PMC8545985 DOI: 10.3389/fvets.2021.707368] [Citation(s) in RCA: 12] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/10/2021] [Accepted: 09/02/2021] [Indexed: 12/23/2022] Open
Abstract
Recent studies demonstrated that domestic cats can be naturally and experimentally infected with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This study was performed to investigate the presence of SARS-CoV-2-specific antibodies within the domestic cat population in Istanbul, Turkey, before the coronavirus disease 2019 (COVID-19) and during the COVID-19 pandemic. Overall, from 155 cat sera analyzed, 26.45% (41/155) tested positive in the spike protein-ELISA (S-ELISA), 28.38% (44/155) in the receptor-binding domain-ELISA (RBD-ELISA), and 21.9% (34/155) in both, the S- and RBD-ELISAs. Twenty-seven of those were also positive for the presence of antibodies to feline coronavirus (FCoV). Among the 34 SARS-CoV-2-positive sera, three of those were positive on serum neutralization assay. Six of the 30 cats before COVID-19 and 28 of the 125 cats during COVID-19 were found to be seropositive. About 20% of ELISA-positive cats exhibited mainly respiratory, gastrointestinal, and renal signs and skin lesions. Hematocrit, hemoglobin, white blood cells, lymphocyte, and platelet numbers were low in about 30% of ELISA-positive cats. The number of neutrophils and monocytes were above normal values in about 20% of ELISA-positive cats. The liver enzyme alanine aminotransferase levels were high in 23.5% ELISA-positive cats. In conclusion, this is the first report describing antibodies specific to SARS-CoV-2 antigens (S and RBD) in cats in Istanbul, Turkey, indicating the risk for domestic cats to contract SARS-CoV-2 from owners and/or household members with COVID-19. This study and others show that COVID-19-positive pet owners should limit their contact with companion animals and that pets with respiratory signs should be monitored for SARS-CoV-2 infections.
Collapse
Affiliation(s)
- Aysun Yilmaz
- Department of Virology, Veterinary Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey
| | - Abdullah Kayar
- Department of Internal Medicine, Veterinary Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey
| | - Nuri Turan
- Department of Virology, Veterinary Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey
| | - Onur Iskefli
- Department of Internal Medicine, Veterinary Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey
| | - Alper Bayrakal
- Department of Internal Medicine, Veterinary Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey
| | - Gleyder Roman-Sosa
- Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, United States
| | - Erman Or
- Department of Internal Medicine, Veterinary Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey
| | - Hasan Emre Tali
- Department of Virology, Veterinary Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey
| | - Bekir Kocazeybek
- Department of Medical Microbiology, Cerrahpasa Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
| | - Ridvan Karaali
- Department of Infectious Diseases and Clinical Microbiology, Cerrahpasa Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
| | - Dashzeveg Bold
- Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, United States
| | - Jean-Remy Sadeyen
- Avian Influenza Group, The Pirbright Institute, Woking, United Kingdom
| | | | - Pengxiang Chang
- Avian Influenza Group, The Pirbright Institute, Woking, United Kingdom
| | - Munir Iqbal
- Avian Influenza Group, The Pirbright Institute, Woking, United Kingdom
| | - Juergen A. Richt
- Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, United States
| | - Huseyin Yilmaz
- Department of Virology, Veterinary Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey
| |
Collapse
|
|
47
|
Guerrini A. Animals, vaccines, and COVID-19. ENDEAVOUR 2021; 45:100779. [PMID: 34352721 PMCID: PMC8289630 DOI: 10.1016/j.endeavour.2021.100779] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 04/30/2021] [Revised: 07/05/2021] [Accepted: 07/15/2021] [Indexed: 06/13/2023]
Abstract
Animals, especially mammals, have played a critical role in the COVID-19 pandemic. The COVID-19 virus originated in animals, and the virus can jump back and forth between humans and animals. Moreover, animals have been central to the development of the various vaccines against the virus now employed around the world, continuing a long history. The interrelationships between animals and humans in both disease transmission and its prevention call for an interdisciplinary approach to medicine.
Collapse
Affiliation(s)
- Anita Guerrini
- Oregon State University, 322 Milam Hall, Corvallis, OR 97331, USA.
| |
Collapse
|
|
48
|
Bonilla-Aldana DK, García-Barco A, Jimenez-Diaz SD, Bonilla-Aldana JL, Cardona-Trujillo MC, Muñoz-Lara F, Zambrano LI, Salas-Matta LA, Rodriguez-Morales AJ. SARS-CoV-2 natural infection in animals: a systematic review of studies and case reports and series. Vet Q 2021; 41:250-267. [PMID: 34406913 PMCID: PMC8428274 DOI: 10.1080/01652176.2021.1970280] [Citation(s) in RCA: 18] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/23/2022] Open
Abstract
COVID-19 pandemic is essentially a zoonotic disease. In this context, early in 2020, transmission from humans to certain animals began reporting; the number of studies has grown since. To estimate the pooled prevalence of SARS-CoV-2 natural infection in animals and to determine differences in prevalence between countries, years, animal types and diagnostic methods (RT-PCR or serological tests). A systematic literature review with meta-analysis using eight databases. Observational studies were included but analyzed separately. We performed a random-effects model meta-analysis to calculate the pooled prevalence and 95% confidence interval (95% CI) for prevalence studies and case series. After the screening, 65 reports were selected for full-text assessment and included for qualitative and quantitative analyses. A total of 24 reports assessed SARS-CoV-2 infection by RT-PCR, combining a total of 321,785 animals, yielding a pooled prevalence of 12.3% (95% CI 11.6%–13.0%). Also, a total of 17 studies additionally assessed serological response against SARS-CoV-2, including nine by ELISA, four by PRTN, one by MIA, one by immunochromatography (rest, two studies, the method was not specified), combining a total of 5319 animals, yielding a pooled prevalence of 29.4% (95% CI 22.9%–35.9%). A considerable proportion of animals resulted infected by SARS-CoV-2, ranking minks among the highest value, followed by dogs and cats. Further studies in other animals are required to define the extent and importance of natural infection due to SARS-CoV-2. These findings have multiple implications for public human and animal health. One Health approach in this context is critical for prevention and control.
Collapse
Affiliation(s)
- D Katterine Bonilla-Aldana
- Semillero de Investigación en Zoonosis (SIZOO), Grupo de Investigación GISCA, Fundación Universitaria Autónoma de las Américas, Pereira, Risaralda, Colombia
| | - Alejandra García-Barco
- Grupo Colaborativo de Investigación en Enfermedades Transmitidas por vectores, Zoonóticas y tropicales de Risaralda, Pereira, Risaralda, Colombia
| | - S Daniela Jimenez-Diaz
- Semillero de Investigación en Zoonosis (SIZOO), Grupo de Investigación GISCA, Fundación Universitaria Autónoma de las Américas, Pereira, Risaralda, Colombia
| | - Jorge Luis Bonilla-Aldana
- School of Veterinary Medicine and Zootechnics, Universidad de la Amazonia, Florencia, Caquetá, Colombia
| | - Maria C Cardona-Trujillo
- Grupo Colaborativo de Investigación en Enfermedades Transmitidas por vectores, Zoonóticas y tropicales de Risaralda, Pereira, Risaralda, Colombia
| | - Fausto Muñoz-Lara
- Department of Internal Medicine, Faculty of Medical Sciences, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras.,Department of Internal Medicine, Hospital Escuela, Tegucigalpa, Honduras
| | - Lysien I Zambrano
- Unit of Scientific Research, School of Medicine, Faculty of Medical Sciences, Universidad Nacional Autónoma de Honduras (UNAH), Tegucigalpa, Honduras
| | | | - Alfonso J Rodriguez-Morales
- Faculty of Health Sciences, Universidad Científica del Sur, Lima, Perú.,Grupo de Investigación Biomedicina, Faculty of Medicine, Fundación Universitaria Autónoma de las Américas, Pereira, Risaralda, Colombia.,School of Medicine, Universidad Privada Franz Tamayo (UNIFRANZ), Cochabamba, Bolivia
| |
Collapse
|
|
49
|
Boraschi P, Giugliano L, Mercogliano G, Donati F, Romano S, Neri E. Abdominal and gastrointestinal manifestations in COVID-19 patients: Is imaging useful? World J Gastroenterol 2021; 27:4143-4159. [PMID: 34326615 PMCID: PMC8311532 DOI: 10.3748/wjg.v27.i26.4143] [Citation(s) in RCA: 20] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/26/2021] [Revised: 04/08/2021] [Accepted: 04/21/2021] [Indexed: 02/06/2023] Open
Abstract
Coronavirus disease 2019 (COVID-19) can be considered a systemic disease with a specific tropism for the vascular system, in which the alterations of the microcirculation have an important pathogenetic role. The lungs are the main organ involved in COVID-19, and severe progressive respiratory failure is the leading cause of death in the affected patients; however, many other organs can be involved with variable clinical manifestations. Concerning abdominal manifestations, the gastrointestinal tract and the hepatobiliary system are mainly affected, although the pancreas, urinary tract and spleen may also be involved. The most common gastrointestinal symptoms are loss of appetite, followed by nausea and vomiting, diarrhea and abdominal pain. Gastrointestinal imaging findings include bowel wall thickening, sometimes associated with hyperemia and mesenteric thickening, fluid-filled segments of the large bowel and rarely intestinal pneumatosis and ischemia. Hepatic involvement manifests as an increase in the enzymatic levels of alanine aminotransferase, aspartate aminotransferase, serum bilirubin and γ-glutamyl transferase with clinical manifestations in most cases mild and transient. The most frequent radiological features are hepatic steatosis, biliary sludge and gallstones. Edematous acute pancreatitis, kidney infarct and acute kidney injury from acute tubular necrosis have been described more rarely in COVID-19. Lastly, splenic involvement is characterized by splenomegaly and by the development of solitary or multifocal splenic infarcts with classic wedge-shaped or even rounded morphology, with irregular or smooth profiles. In summary, the abdominal radiological findings of COVID-19 are nonspecific and with poor pathological correlation reported in the literature. Ultrasound and particularly computed tomography with multiphasic acquisition are the diagnostic methods mainly utilized in COVID-19 patients with abdominal clinical symptoms and signs. Although radiological signs are not specific of abdominal and gastrointestinal involvement, the diagnostic imaging modalities and in particular computed tomography are helpful for the clinician in the management, evaluation of the severity and evolution of the COVID-19 patients.
Collapse
Affiliation(s)
- Piero Boraschi
- Department of Diagnostic Imaging, Second Division of Radiology, Azienda Ospedaliero-Universitaria Pisana-University of Pisa, Pisa 56124, Italy
| | - Luigi Giugliano
- Department of Radiology, University of Naples “Federico II”, Naples 80131, Italy
| | - Giuseppe Mercogliano
- Department of Radiology, University of Naples “Federico II”, Naples 80131, Italy
| | - Francescamaria Donati
- Department of Diagnostic Imaging, Second Division of Radiology, Azienda Ospedaliero-Universitaria Pisana-University of Pisa, Pisa 56124, Italy
| | - Stefania Romano
- Department of Diagnostic Imaging, Santa Maria delle Grazie Hospital, Naples 80078, Italy
| | - Emanuele Neri
- Diagnostic and Interventional Radiology, Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Pisa 56126, Italy
| |
Collapse
|
|
50
|
Giraldo-Ramirez S, Rendon-Marin S, Jaimes JA, Martinez-Gutierrez M, Ruiz-Saenz J. SARS-CoV-2 Clinical Outcome in Domestic and Wild Cats: A Systematic Review. Animals (Basel) 2021; 11:2056. [PMID: 34359182 PMCID: PMC8300124 DOI: 10.3390/ani11072056] [Citation(s) in RCA: 21] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/03/2021] [Revised: 06/21/2021] [Accepted: 06/23/2021] [Indexed: 12/13/2022] Open
Abstract
Recently, it has been proved that SARS-CoV-2 has the ability to infect multiple species. This work was aimed at identifying the clinical signs of SARS-CoV-2 infection in domestic and wild felids. A PRISMA-based systematic review was performed on case reports on domestic and wild cats, reports on experimental infections, case reports in databases, preprints and published press releases. Descriptive statistical analysis of the data was performed. A total of 256 articles, 63 detailed official reports and 2 press articles on SARS-CoV-2 infection in domestic and wild cats were analyzed, of which 19 articles and 65 reports were finally included. In domestic cats, most cats' infections are likely to be asymptomatic, and 46% of the reported infected animals were symptomatic and predominantly presented respiratory signs such as sneezing and coughing. In wild felines, respiratory clinical signs were most frequent, and up to 96.5% of the reported affected animals presented coughing. It is noteworthy that, to date, symptomatic animals with SARS-CoV-2 infection have been reported to belong to two different subfamilies (Phanterinae and Felinae), with up to five different felid species affected within the Felidae family. Reported results evince that the signs developed in felids show similar progression to those occurring in humans, suggesting a relationship between the viral cycle and target tissues of the virus in different species. While viral transmission to humans in contact with animal populations has not been reported, spill-back could result in the emergence of immune-escape mutants that might pose a risk to public health. Despite the clear results in the identification of the typical clinical picture of SARS-CoV-2 infection in felines, the number of detailed academic reports and papers on the subject is scarce. Therefore, further description of these cases will allow for more accurate and statistically robust clinical approaches in the future.
Collapse
Affiliation(s)
- Sebastian Giraldo-Ramirez
- Grupo de Investigación en Ciencias Animales—GRICA, Facultad de Medicina Veterinaria y Zootecnia, Universidad Cooperativa de Colombia, Bucaramanga 680002, Colombia; (S.G.-R.); (S.R.-M.); (M.M.-G.)
| | - Santiago Rendon-Marin
- Grupo de Investigación en Ciencias Animales—GRICA, Facultad de Medicina Veterinaria y Zootecnia, Universidad Cooperativa de Colombia, Bucaramanga 680002, Colombia; (S.G.-R.); (S.R.-M.); (M.M.-G.)
| | - Javier A. Jaimes
- Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA;
| | - Marlen Martinez-Gutierrez
- Grupo de Investigación en Ciencias Animales—GRICA, Facultad de Medicina Veterinaria y Zootecnia, Universidad Cooperativa de Colombia, Bucaramanga 680002, Colombia; (S.G.-R.); (S.R.-M.); (M.M.-G.)
- Infettare, Facultad de Medicina, Universidad Cooperativa de Colombia, Medellín 050012, Colombia
| | - Julian Ruiz-Saenz
- Grupo de Investigación en Ciencias Animales—GRICA, Facultad de Medicina Veterinaria y Zootecnia, Universidad Cooperativa de Colombia, Bucaramanga 680002, Colombia; (S.G.-R.); (S.R.-M.); (M.M.-G.)
| |
Collapse
|