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For: Killian JK, Walker RL, Bilke S, Chen Y, Davis S, Cornelison R, Smith WI, Meltzer PS. Genome-wide methylation profiling in archival formalin-fixed paraffin-embedded tissue samples. Methods Mol Biol 2012. [PMID: 22081342 DOI: 10.1007/978-1-60327-216-2-8] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [What about the content of this article? (0)] [Subscribe] [Scholar Register] [Indexed: 12/22/2022]

For:	Killian JK, Walker RL, Bilke S, Chen Y, Davis S, Cornelison R, Smith WI, Meltzer PS. Genome-wide methylation profiling in archival formalin-fixed paraffin-embedded tissue samples. Methods Mol Biol 2012. [PMID: 22081342 DOI: 10.1007/978-1-60327-216-2-8] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [What about the content of this article? (0)] [Subscribe] [Scholar Register] [Indexed: 12/22/2022]

Number	Cited by Other Article(s)
1	Tadic M, Stoos-Veic T, Kusec R. Endoscopic ultrasound guided fine needle aspiration and useful ancillary methods. World J Gastroenterol 2014;20:14292-14300. [PMID: 25339816 PMCID: PMC4202358 DOI: 10.3748/wjg.v20.i39.14292] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/28/2013] [Revised: 03/22/2014] [Accepted: 07/11/2014] [Indexed: 02/06/2023] Open Abstract The role of endoscopic ultrasound (EUS) in evaluating pancreatic pathology has been well documented from the beginning of its clinical use. High spatial resolution and the close proximity to the evaluated organs within the mediastinum and abdominal cavity allow detection of small focal lesions and precise tissue acquisition from suspected lesions within the reach of this method. Fine needle aspiration (FNA) is considered of additional value to EUS and is performed to obtain tissue diagnosis. Tissue acquisition from suspected lesions for cytological or histological analysis allows, not only the differentiation between malignant and non-malignant lesions, but, in most cases, also the accurate distinction between the various types of malignant lesions. It is well documented that the best results are achieved only if an adequate sample is obtained for further analysis, if the material is processed in an appropriate way, and if adequate ancillary methods are performed. This is a multi-step process and could be quite a challenge in some cases. In this article, we discuss the technical aspects of tissue acquisition by EUS-guided-FNA (EUS-FNA), as well as the role of an on-site cytopathologist, various means of specimen processing, and the selection of the appropriate ancillary method for providing an accurate tissue diagnosis and maximizing the yield of this method. The main goal of this review is to alert endosonographers, not only to the different possibilities of tissue acquisition, namely EUS-FNA, but also to bring to their attention the importance of proper sample processing in the evaluation of various lesions in the gastrointestinal tract and other accessible organs. All aspects of tissue acquisition (needles, suction, use of stylet, complications, etc.) have been well discussed lately. Adequate tissue samples enable comprehensive diagnoses, which answer the main clinical questions, thus enabling targeted therapy. Collapse Key Words Endoscopic ultrasound Endoscopic ultrasound-guided fine needle aspiration Endoscopic ultrasound-guided tissue acquisition Fine needle aspiration cytology Ancillary studies Molecular testing Flow cytometry immunophenotyping Collapse MESH Headings Biomarkers/analysis Cytogenetic Analysis Endoscopic Ultrasound-Guided Fine Needle Aspiration/adverse effects Endoscopic Ultrasound-Guided Fine Needle Aspiration/instrumentation Endoscopic Ultrasound-Guided Fine Needle Aspiration/methods Flow Cytometry Humans Immunohistochemistry Immunophenotyping Molecular Diagnostic Techniques Predictive Value of Tests Reproducibility of Results Collapse Grants Collapse Affiliation(s) Collapse

Number

Cited by Other Article(s)

Tadic M, Stoos-Veic T, Kusec R. Endoscopic ultrasound guided fine needle aspiration and useful ancillary methods. World J Gastroenterol 2014;20:14292-14300. [PMID: 25339816 PMCID: PMC4202358 DOI: 10.3748/wjg.v20.i39.14292] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/28/2013] [Revised: 03/22/2014] [Accepted: 07/11/2014] [Indexed: 02/06/2023] Open

Abstract

The role of endoscopic ultrasound (EUS) in evaluating pancreatic pathology has been well documented from the beginning of its clinical use. High spatial resolution and the close proximity to the evaluated organs within the mediastinum and abdominal cavity allow detection of small focal lesions and precise tissue acquisition from suspected lesions within the reach of this method. Fine needle aspiration (FNA) is considered of additional value to EUS and is performed to obtain tissue diagnosis. Tissue acquisition from suspected lesions for cytological or histological analysis allows, not only the differentiation between malignant and non-malignant lesions, but, in most cases, also the accurate distinction between the various types of malignant lesions. It is well documented that the best results are achieved only if an adequate sample is obtained for further analysis, if the material is processed in an appropriate way, and if adequate ancillary methods are performed. This is a multi-step process and could be quite a challenge in some cases. In this article, we discuss the technical aspects of tissue acquisition by EUS-guided-FNA (EUS-FNA), as well as the role of an on-site cytopathologist, various means of specimen processing, and the selection of the appropriate ancillary method for providing an accurate tissue diagnosis and maximizing the yield of this method. The main goal of this review is to alert endosonographers, not only to the different possibilities of tissue acquisition, namely EUS-FNA, but also to bring to their attention the importance of proper sample processing in the evaluation of various lesions in the gastrointestinal tract and other accessible organs. All aspects of tissue acquisition (needles, suction, use of stylet, complications, etc.) have been well discussed lately. Adequate tissue samples enable comprehensive diagnoses, which answer the main clinical questions, thus enabling targeted therapy.

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