Histopathological confirmation of similar intramucosal distribution of fluorescein in both intravenous administration and local mucosal application for probe-based confocal laser endomicroscopy of the normal stomach

Figure 5 Histology 5 min after intravenous fluorescein administration. A: HE staining of biopsied tissue (original magnification × 200); B: Fluorescence observed in the serial section of Figure 5A. Green fluorescence in the cytoplasm of fundic glandular cells is more clearly observed compared with that at one minute (non-HE staining; original magnification × 200); C: The region in the red square is magnified. Green fluorescence is noted around the nuclei (light blue) of fundic glandular cells, suggesting that fluorescein was incorporated into the cytoplasm of the cells.