Copyright
©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. May 16, 2022; 10(14): 4601-4607
Published online May 16, 2022. doi: 10.12998/wjcc.v10.i14.4601
Published online May 16, 2022. doi: 10.12998/wjcc.v10.i14.4601
Diagnosis of cytomegalovirus encephalitis using metagenomic next-generation sequencing of blood and cerebrospinal fluid: A case report
Chang-Qing Xu, Xia-Ling Chen, Dong-Sheng Zhang, Jia-Wei Wang, Hong Yuan, Wei-Fan Chen, Zhong-Yin Zhang, Department of Neurology, Dongguan Kanghua Hospital, Dongguan 523080, Guangdong Province, China
Han Xia, Department of Scientific Affairs, Hugobiotech Co., Ltd., Beijing 100176, China
Fu-Hua Peng, Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China
Author contributions: Peng FH, Zhang ZY, and Xu CQ directly participated in planning and execution; Xu CQ, Chen XL, Zhang DS, Wang JW, Yuan H, and Chen WF collected and organized the data; Lou Z and Xia H contributed in metagenomic next-generation sequencing experiment and result analysis; Peng FH, Xu CQ, Chen XL, and Zhang DS analyzed the data; Xu CQ, Chen XL, Zhang DS, and Wang JW drafted and revised the manuscript; all the authors read and approved the final manuscript.
Informed consent statement: This study was approved by the ethical review committee of First Affiliated Hospital of Anhui Medical University. Written informed consent was obtained from the patient.
Conflict-of-interest statement: All authors declare that they have no conflicts of interest.
CARE Checklist (2016) statement: The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Fu-Hua Peng, PhD, Doctor, Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China. pengfh@mail.sysu.edu.cn
Received: October 15, 2021
Peer-review started: October 15, 2021
First decision: December 17, 2021
Revised: December 23, 2021
Accepted: March 25, 2022
Article in press: March 25, 2022
Published online: May 16, 2022
Peer-review started: October 15, 2021
First decision: December 17, 2021
Revised: December 23, 2021
Accepted: March 25, 2022
Article in press: March 25, 2022
Published online: May 16, 2022
Core Tip
Core Tip: Cytomegalovirus infection in brain tissue is rare, and the diagnosis is limited. Here we report a case of cytomegalovirus encephalitis that was diagnosed by metagenomic next-generation sequencing using blood and cerebrospinal fluid samples. This indicated that metagenomic next-generation sequencing could be a reliable approach for the diagnosis of cytomegalovirus encephalitis with high efficiency, sensitivity, and specificity.