New categorization of human vascular endothelial cells by pro- vs anti-proliferative phenotypes

Figure 5 Phenotype evaluation of human induced pluripotent stem cells-derived vascular endothelial cells. A and B: VSMC-co-culture experiments of VECs generated from three lines of Ret-iPSCs (#25, 253G1 and 201B7); A and those from two lines of SeV-iPSCs(SeV-iPS(BJ) and [SeV-iPS(HUVEC)]; B: At indicated passage number (n = 3); C: RT-PCR of RGS5 message of Ret-iPSdECs (type-I) and SeV-iPSdECs (type-II); D: RGS5 immunostaining studies of Ret-iPSdECs (upper) and SeV-iPSdECs (lower); E: H₂O₂-treating experiments were performed in type-II SeV-iPSdECs as in Figure 4E. VSMC: Vascular smooth muscle cell; VEC: Vascular endothelial cell; iPSC: Induced pluripotent stem cell; PCR: Polymerase chain reaction; RGS5: Regulator of G-protein signaling 5; HUVEC: Human umbilical vein endothelial cell.