Epigenetics and DNA methylation in cancer

Figure 9 Methods for genomic DNA methylation analysis, classified as global, locus-specific and genome-wide. In the case of locus-specific approaches, techniques were divided depending on the use of methylation-specific primers or not. RP-HPLC: Reverse-Phase high-performance liquid chromatography; HPCE: High performance capillary electrophoresis; Sss1 assay: Methyl group acceptance assay; MSP: Methylation-specific polymerase chain reaction (PCR); MALDI-TOF-MS: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; MCA: Methylation CpG island amplification; DMH: Differential methylation hybridization; RLGS: Restriction-landmark genomic scanning; AIMS: Amplification of inter-methylated sites; MSDK: Methylation-specific digital karyotyping; HTF-HELP: HPAII tiny fragment enrichment by ligation-mediated PCR; MBD-column: Methylated DNA binding column; MIRA: Methylated CpG island recovery assay; MeDIP-seq: Methyl-DNA immunoprecipitation and sequencing; RRBS: Reduced Representation Bisulfite Sequencing. Adapted by Toraño et al[33], 2011.