Nuclear factor κB represses the expression of latent membrane protein 1 in Epstein-Barr virus transformed cells

Figure 3 Latent membrane protein 1 negatively regulates its own promoter activity. A: Schematic diagram of Epstein-Barr virus (EBV) latent membrane protein-1 (LMP1) promoter reporter constructs. RNA start site is shown. The drawing is not to scale; B: 293T cells were transfected with LMP1 promoter reporter construct and expression plasmid (0.01, 0.05, and 0.1 μg) as shown at the top. The LMP-DM has mutations in the critical domains in LMP1 for signaling. Cell lysates were used for the luciferase and β-galactosidase assays. Relative promoter reporter activities (luciferase/β-galactosidase) are shown. The results represented an average of triplicate transfections; Standard error bars are shown. Results from one representative experiment are of shown. The statistically significant difference between the indicated samples is denoted as ^aP < 0.05; ^bP < 0.01; C: 293T cells were transfected with the indicated plasmid as shown at the top. Nuclear factor κB (NF-κB) specific reporter construct was used. One day later, luciferase and β-galactosidase activities were measured. Relative promoter reporter activities are shown.